HM43_CAEEL
ID HM43_CAEEL Reviewed; 273 AA.
AC Q18273;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Homeobox protein ceh-43 {ECO:0000312|WormBase:C28A5.4a};
GN Name=ceh-43; Synonyms=dopy-2 {ECO:0000312|WormBase:C28A5.4a};
GN ORFNames=C28A5.4 {ECO:0000312|WormBase:C28A5.4a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11747075; DOI=10.1002/dvdy.1201;
RA Aspock G., Buerglin T.R.;
RT "The Caenorhabditis elegans distal-less ortholog ceh-43 is required for
RT development of the anterior hypodermis.";
RL Dev. Dyn. 222:403-409(2001).
RN [3]
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF ARG-103.
RX PubMed=23788625; DOI=10.1101/gad.217224.113;
RA Doitsidou M., Flames N., Topalidou I., Abe N., Felton T., Remesal L.,
RA Popovitchenko T., Mann R., Chalfie M., Hobert O.;
RT "A combinatorial regulatory signature controls terminal differentiation of
RT the dopaminergic nervous system in C. elegans.";
RL Genes Dev. 27:1391-1405(2013).
CC -!- FUNCTION: Probable transcription factor (PubMed:23788625). Binds to the
CC sequence motif 5'-ATAAT-3' in regulatory elements (PubMed:23788625).
CC Required for development of the anterior hypodermis during embryonic
CC morphogenesis for cell adhesion; also affects embryonic and larval
CC viability (PubMed:11747075). Modulates and maintains dopaminergic
CC neuron differentiation (PubMed:23788625). May activate dopamine pathway
CC genes in concert with ETS domain-containing protein ast-1, and homeobox
CC proteins ceh-40 and ceh-20 (PubMed:23788625).
CC {ECO:0000269|PubMed:11747075, ECO:0000269|PubMed:23788625}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the head hypdodermis,
CC neuronal support cells and CAN neurons. {ECO:0000269|PubMed:11747075}.
CC -!- DEVELOPMENTAL STAGE: Expressed in dopaminergic neurons throughout the
CC life of the neurons. {ECO:0000269|PubMed:23788625}.
CC -!- SIMILARITY: Belongs to the distal-less homeobox family. {ECO:0000305}.
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DR EMBL; BX284603; CAA83601.1; -; Genomic_DNA.
DR PIR; C88429; C88429.
DR PIR; S43577; S43577.
DR RefSeq; NP_497904.1; NM_065503.3.
DR AlphaFoldDB; Q18273; -.
DR SMR; Q18273; -.
DR BioGRID; 40817; 23.
DR IntAct; Q18273; 21.
DR STRING; 6239.C28A5.4; -.
DR EPD; Q18273; -.
DR PaxDb; Q18273; -.
DR PeptideAtlas; Q18273; -.
DR EnsemblMetazoa; C28A5.4a.1; C28A5.4a.1; WBGene00000463.
DR GeneID; 175581; -.
DR KEGG; cel:CELE_C28A5.4; -.
DR UCSC; C28A5.4; c. elegans.
DR CTD; 175581; -.
DR WormBase; C28A5.4a; CE05325; WBGene00000463; ceh-43.
DR eggNOG; KOG0850; Eukaryota.
DR GeneTree; ENSGT00940000167651; -.
DR HOGENOM; CLU_1020241_0_0_1; -.
DR InParanoid; Q18273; -.
DR OMA; CEAKYNT; -.
DR PhylomeDB; Q18273; -.
DR SignaLink; Q18273; -.
DR PRO; PR:Q18273; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00000463; Expressed in embryo and 3 other tissues.
DR ExpressionAtlas; Q18273; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IMP:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IMP:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007155; P:cell adhesion; TAS:WormBase.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:UniProtKB.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..273
FT /note="Homeobox protein ceh-43"
FT /id="PRO_0000049056"
FT DNA_BIND 102..161
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 47..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 153..204
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..67
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..204
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 103
FT /note="R->K: In ot406; significantly more pronounced defect
FT in the expression of dopamine pathway genes in adults
FT compared with young larvae. Progressive loss of expression
FT of the dopamine transporter dat-1 in the ADE and CEPD
FT neurons; effect is exacerbated, either in the ast-1 mutant
FT background or, in a combined ceh-20 and ceh-40 mutant
FT background."
FT /evidence="ECO:0000269|PubMed:23788625"
SQ SEQUENCE 273 AA; 30163 MW; 18B93FF08C2576EB CRC64;
MDPSKGFEYV AGDYYQTSGV APPTSNGAGS NVSPYFPYHA YPTSSTNGAT GGSMYGTPQQ
TSAYAMYPPG PGSSPEEAFP EHTTTKIVEG CEAKYNVKGK KMRKPRTIYN SSQLQMLQKK
FQKTQYLALP DRAALAHELG LSQTQVKIWF QNRRSKQKKQ KGGSSDHASD EEDDDTEESK
PESPPMGESV MIQESSEPRT LVSSSIKTEM KEEYPPMTLN EQYASPYLYG SDFSTILPPS
QGFPNNALYN TAGAYPSIDY TNGVYQNTLY KYV
