HMA3_ORYSJ
ID HMA3_ORYSJ Reviewed; 1004 AA.
AC Q8H384; E3WCP0; Q0D7L9;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Cadmium/zinc-transporting ATPase HMA3 {ECO:0000305};
DE EC=7.2.2.12 {ECO:0000305|PubMed:20823253};
DE EC=7.2.2.21 {ECO:0000269|PubMed:20823253};
DE AltName: Full=Protein HEAVY METAL ATPASE 3 {ECO:0000305};
DE Short=OsHMA3 {ECO:0000303|PubMed:20823253};
GN Name=HMA3 {ECO:0000303|PubMed:20823253}; Synonyms=CASTLE1n;
GN OrderedLocusNames=Os07g0232900 {ECO:0000312|EMBL:BAF21154.2},
GN LOC_Os07g12900 {ECO:0000305};
GN ORFNames=OSJNBa0061L20.105 {ECO:0000312|EMBL:BAC21509.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, POLYMORPHISM, AND MUTAGENESIS OF ARG-80.
RC STRAIN=cv. Nipponbare;
RX PubMed=20823253; DOI=10.1073/pnas.1005396107;
RA Ueno D., Yamaji N., Kono I., Huang C.F., Ando T., Yano M., Ma J.F.;
RT "Gene limiting cadmium accumulation in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:16500-16505(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Akita 63;
RX PubMed=20840506; DOI=10.1111/j.1469-8137.2010.03459.x;
RA Miyadate H., Adachi S., Hiraizumi A., Tezuka K., Nakazawa N., Kawamoto T.,
RA Katou K., Kodama I., Sakurai K., Takahashi H., Satoh-Nagasawa N.,
RA Watanabe A., Fujimura T., Akagi H.;
RT "OsHMA3, a P1B-type of ATPase affects root-to-shoot cadmium translocation
RT in rice by mediating efflux into vacuoles.";
RL New Phytol. 189:190-199(2011).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP FUNCTION, AND BIOTECHNOLOGY.
RX PubMed=25151617; DOI=10.1093/jxb/eru340;
RA Sasaki A., Yamaji N., Ma J.F.;
RT "Overexpression of OsHMA3 enhances Cd tolerance and expression of Zn
RT transporter genes in rice.";
RL J. Exp. Bot. 65:6013-6021(2014).
CC -!- FUNCTION: Root-specific cadmium (Cd) transporter that mediates Cd
CC efflux in root vacuoles. Involved in Cd detoxification by sequestrating
CC Cd into root vacuoles and limiting translocation of Cd from the roots
CC to the shoots, and accumulation in grains.
CC {ECO:0000269|PubMed:20823253, ECO:0000269|PubMed:20840506,
CC ECO:0000269|PubMed:25151617}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Zn(2+)(in) = ADP + H(+) + phosphate + Zn(2+)(out);
CC Xref=Rhea:RHEA:20621, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29105, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=7.2.2.12;
CC Evidence={ECO:0000305|PubMed:20823253};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + Cd(2+)(in) + H2O = ADP + Cd(2+)(out) + H(+) + phosphate;
CC Xref=Rhea:RHEA:12132, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:48775,
CC ChEBI:CHEBI:456216; EC=7.2.2.21;
CC Evidence={ECO:0000269|PubMed:20823253};
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:20823253,
CC ECO:0000269|PubMed:20840506}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Localizes to the tonoplast of root cells.
CC {ECO:0000269|PubMed:20823253, ECO:0000269|PubMed:20840506}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in roots.
CC {ECO:0000269|PubMed:20823253, ECO:0000269|PubMed:20840506}.
CC -!- POLYMORPHISM: A single amino acid substitution of Arg-80 to His
CC abolishes HMA3 cadmium transport activity through the tonoplast in root
CC cells. This allele is found in a number of rice cultivars, and is
CC associated with high accumulation of cadmium in rice grains.
CC Identification of natural allelic variation in HMA3 may facilitate the
CC development of rice varieties with grain cadmium concentrations adapted
CC to dietary needs in function of the cadmium concentration in soil.
CC {ECO:0000269|PubMed:20823253}.
CC -!- BIOTECHNOLOGY: Over-expression of HMA3 allows to reduce efficiently
CC cadmium accumulation in the grain and to enhance cadmium tolerance in
CC rice. {ECO:0000269|PubMed:25151617}.
CC -!- SIMILARITY: Belongs to the cation transport ATPase (P-type) (TC 3.A.3)
CC family. Type IB subfamily. {ECO:0000305}.
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DR EMBL; AB557931; BAJ23048.1; -; mRNA.
DR EMBL; AB559519; BAJ25742.1; -; mRNA.
DR EMBL; AB559522; BAJ25745.1; -; Genomic_DNA.
DR EMBL; AP005246; BAC21509.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21154.2; -; Genomic_DNA.
DR EMBL; AP014963; BAT00724.1; -; Genomic_DNA.
DR RefSeq; XP_015647368.1; XM_015791882.1.
DR AlphaFoldDB; Q8H384; -.
DR SMR; Q8H384; -.
DR STRING; 4530.OS07T0232900-01; -.
DR TCDB; 3.A.3.6.17; the p-type atpase (p-atpase) superfamily.
DR PaxDb; Q8H384; -.
DR PRIDE; Q8H384; -.
DR EnsemblPlants; Os07t0232900-01; Os07t0232900-01; Os07g0232900.
DR GeneID; 4342783; -.
DR Gramene; Os07t0232900-01; Os07t0232900-01; Os07g0232900.
DR KEGG; osa:4342783; -.
DR eggNOG; KOG0207; Eukaryota.
DR HOGENOM; CLU_001771_6_1_1; -.
DR InParanoid; Q8H384; -.
DR OMA; FAQPWET; -.
DR OrthoDB; 649559at2759; -.
DR BRENDA; 7.2.2.2; 4460.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008551; F:P-type cadmium transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0016463; F:P-type zinc transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IBA:GO_Central.
DR Gene3D; 3.40.1110.10; -; 1.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR023299; ATPase_P-typ_cyto_dom_N.
DR InterPro; IPR018303; ATPase_P-typ_P_site.
DR InterPro; IPR023298; ATPase_P-typ_TM_dom_sf.
DR InterPro; IPR008250; ATPase_P-typ_transduc_dom_A_sf.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR006121; HMA_dom.
DR InterPro; IPR036163; HMA_dom_sf.
DR InterPro; IPR027256; P-typ_ATPase_IB.
DR InterPro; IPR001757; P_typ_ATPase.
DR InterPro; IPR044492; P_typ_ATPase_HD_dom.
DR Pfam; PF00403; HMA; 1.
DR SFLD; SFLDF00027; p-type_atpase; 1.
DR SUPFAM; SSF55008; SSF55008; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR SUPFAM; SSF81653; SSF81653; 1.
DR SUPFAM; SSF81665; SSF81665; 1.
DR TIGRFAMs; TIGR01525; ATPase-IB_hvy; 1.
DR TIGRFAMs; TIGR01494; ATPase_P-type; 1.
DR PROSITE; PS00154; ATPASE_E1_E2; 1.
DR PROSITE; PS50846; HMA_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cadmium; Ion transport; Membrane; Metal-binding;
KW Nucleotide-binding; Reference proteome; Translocase; Transmembrane;
KW Transmembrane helix; Transport; Vacuole; Zinc.
FT CHAIN 1..1004
FT /note="Cadmium/zinc-transporting ATPase HMA3"
FT /id="PRO_0000440963"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 683..703
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 707..727
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 42..108
FT /note="HMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT REGION 931..952
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 80
FT /note="R->H: Abolishes HMA3 cadmium transport activity
FT through the tonoplast in root cells."
FT /evidence="ECO:0000269|PubMed:20823253"
FT CONFLICT 713
FT /note="L -> F (in Ref. 2; BAJ25745)"
FT /evidence="ECO:0000305"
FT CONFLICT 853
FT /note="G -> A (in Ref. 2; BAJ25745)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1004 AA; 102664 MW; 610C1303317657F3 CRC64;
MAGKDEAEGL EARLLLLPPE AAAEEPTRCG GGDGGGGGRK RKKTYLDVLG VCCSAEVALV
ERLLAPLDGV RVVSVVVASR TVVVEHDPAA APESAIVKAL NKAGLEASVR AYGSSGVVSR
WPSPYIVASG VLLTASFFEW LFPPLQCLAV AAVVAGAPPM VRRGFAAASR LSLDINVLML
IAVAGALCLG DYTEAGAIVF LFTTAEWLET LACTKASAGM SSLMGMLPVK AVIATTGEVV
SVRDVRVGDV VAVRAGEIVP VDGVVVDGQS EVDERSLTGE SFPVPKQPHS EVWAGTMNFD
GYIAVRTTAL AENSTVAKME RLVEAAQNSR SKTQRLIDSC AKYYTPAVVV VAAGVALIPA
LLGADGLEQW WKLALVMLVS ACPCALVLST PVASFCAMLR AARMGIFIKG GDVLESLGEI
RAVAFDKTGT ITRGEFSIDS FHLVGDHKVE MDHLLYWIAS IESKSSHPMA AALVEYAQSK
SIQPNPENVG DFRIYPGEGI YGEIHGKHIY IGNRRTLARA SSPQSTQEMG EMIKGVSIGY
VICDGELAGV FSLSDDCRTG AAEAIRELGS LGIKSVMLTG DSSAAATHAQ GQLGGVMEEL
HSELLPEDKV RLVSGLKARF GPTMMVGDGM NDAAALAAAD VGVSMGISGS AAAMETSHAT
LMSSDVLRVP EAVRLGRCAR RTIAVNVAGS VAVKAAVLAL AAAWRPVLWA AVLADVGTCL
LVVLNSMTLL REEWKGGAKE DGACRATARS LVMRSQLAAD SQAPNAADAG AAGREQTNGC
RCCPKPGMSP EHSVVIDIRA DGERQEERPA EAAVVAKCCG GGGGEGIRCG ASKKPTATVV
VAKCCGGGGG GEGTRCGASK NPATAAVVAK CCSGGGGEGI GCGASKKPTA TAVVAKCCGG
GGEGTRCAAS KKPATAAVVA KCCGGDGGEG TGCGASKRSP PAEGSCSGGE GGTNGVGRCC
TSVKRPTCCD MGAAEVSDSS PETAKDCRNG RCCAKTMNSG EVKG
