HMAL1_YEAST
ID HMAL1_YEAST Reviewed; 175 AA.
AC P0CY07; D6VQV3; P01365;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=Silenced mating-type protein ALPHA1;
DE Short=MATalpha1 protein;
DE AltName: Full=Alpha-1 activator;
GN Name=HMLALPHA1; OrderedLocusNames=YCL066W; ORFNames=YCL66W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7034964; DOI=10.1016/0092-8674(81)90356-1;
RA Astell C.R., Ahlstrom-Jonasson L., Smith M., Tatchell K., Nasmyth K.A.,
RA Hall B.D.;
RT "The sequence of the DNAs coding for the mating-type loci of Saccharomyces
RT cerevisiae.";
RL Cell 27:15-23(1981).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6276023; DOI=10.1016/0092-8674(81)90357-3;
RA Tatchell K., Nasmyth K.A., Hall B.D., Astell C.R., Smith M.;
RT "In vitro mutation analysis of the mating-type locus in yeast.";
RL Cell 27:25-35(1981).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7021055; DOI=10.1101/sqb.1981.045.01.113;
RA Nasmyth K.A., Tatchell K., Hall B.D., Astell C., Smith M.;
RT "Physical analysis of mating-type loci in Saccharomyces cerevisiae.";
RL Cold Spring Harb. Symp. Quant. Biol. 45:961-981(1981).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
RX PubMed=6256656; DOI=10.1038/289244a0;
RA Nasmyth K.A., Tatchell K., Hall B.D., Astell C.R., Smith M.;
RT "A position effect in the control of transcription at yeast mating type
RT loci.";
RL Nature 289:244-250(1981).
CC -!- FUNCTION: Mating type proteins are sequence specific DNA-binding
CC proteins that act as master switches in yeast differentiation by
CC controlling gene expression in a cell type-specific fashion. Silenced
CC copy of ALPHA1 at HML.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00655}.
CC -!- MISCELLANEOUS: There are three genetic loci for mating type genes in
CC S.cerevisiae. MAT is the expression locus that determines the mating
CC type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and
CC HMR (containing HMRA1 and HMRA2) represent silenced repositories of
CC mating type information. The mating type is determined by the MAT
CC locus, which contains either a copy of HML or of HMR. Diploid cells are
CC usually heterozygous for the MAT locus.
CC -!- SIMILARITY: Belongs to the MATALPHA1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00655}.
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DR EMBL; V01315; CAA24626.1; -; Genomic_DNA.
DR EMBL; L00059; AAA34679.1; -; Genomic_DNA.
DR EMBL; X59720; CAA42307.1; -; Genomic_DNA.
DR EMBL; AY557643; AAS55969.1; -; Genomic_DNA.
DR EMBL; V01314; CAA24624.1; -; Genomic_DNA.
DR EMBL; BK006937; DAA07519.1; -; Genomic_DNA.
DR PIR; S19397; JFBYA1.
DR RefSeq; NP_009867.1; NM_001178707.1.
DR RefSeq; NP_009869.3; NM_001178754.1.
DR AlphaFoldDB; P0CY07; -.
DR BioGRID; 30923; 6.
DR BioGRID; 31023; 4.
DR STRING; 4932.YCL066W; -.
DR PaxDb; P0CY07; -.
DR EnsemblFungi; YCL066W_mRNA; YCL066W; YCL066W.
DR EnsemblFungi; YCR040W_mRNA; YCR040W; YCR040W.
DR GeneID; 850293; -.
DR GeneID; 850407; -.
DR KEGG; sce:YCL066W; -.
DR KEGG; sce:YCR040W; -.
DR SGD; S000000571; HMLALPHA1.
DR VEuPathDB; FungiDB:YCL066W; -.
DR VEuPathDB; FungiDB:YCR040W; -.
DR eggNOG; ENOG502S4ZK; Eukaryota.
DR GeneTree; ENSGT00940000176443; -.
DR HOGENOM; CLU_118234_0_0_1; -.
DR InParanoid; P0CY07; -.
DR OMA; MSSTIYC; -.
DR BioCyc; YEAST:G3O-29313-MON; -.
DR PRO; PR:P0CY07; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P0CY07; protein.
DR GO; GO:0005634; C:nucleus; ISA:SGD.
DR GO; GO:0008301; F:DNA binding, bending; ISA:SGD.
DR GO; GO:0003713; F:transcription coactivator activity; ISA:SGD.
DR GO; GO:0045895; P:positive regulation of mating-type specific transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0007532; P:regulation of mating-type specific transcription, DNA-templated; ISA:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISA:SGD.
DR InterPro; IPR006856; MATalpha_HMGbox.
DR Pfam; PF04769; MATalpha_HMGbox; 1.
DR PROSITE; PS51325; ALPHA_BOX; 1.
PE 3: Inferred from homology;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..175
FT /note="Silenced mating-type protein ALPHA1"
FT /id="PRO_0000410462"
FT DNA_BIND 88..144
FT /note="Alpha box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00655"
SQ SEQUENCE 175 AA; 20413 MW; 697199A7686D19C0 CRC64;
MFTSKPAFKI KNKASKSYRN TAVSKKLKEK RLAEHVRPSC FNIIRPLKKD IQIPVPSSRF
LNKIQIHRIA SGSQNTQFRQ FNKTSIKSSK KYLNSFMAFR AYYSQFGSGV KQNVLSSLLA
EEWHADKMQH GIWDYFAQQY NFINPGFGFV EWLTNNYAEV RGDGYWEDVF VHLAL
