HMAL2_YEAST
ID HMAL2_YEAST Reviewed; 210 AA.
AC P0CY09; D6VQV2; P01367; P01368; Q6B2C0;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Silenced mating-type protein ALPHA2;
DE Short=MATalpha2 protein;
DE AltName: Full=Alpha-2 repressor;
GN Name=HMLALPHA2; OrderedLocusNames=YCL067C; ORFNames=YCL67C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7034964; DOI=10.1016/0092-8674(81)90356-1;
RA Astell C.R., Ahlstrom-Jonasson L., Smith M., Tatchell K., Nasmyth K.A.,
RA Hall B.D.;
RT "The sequence of the DNAs coding for the mating-type loci of Saccharomyces
RT cerevisiae.";
RL Cell 27:15-23(1981).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6276023; DOI=10.1016/0092-8674(81)90357-3;
RA Tatchell K., Nasmyth K.A., Hall B.D., Astell C.R., Smith M.;
RT "In vitro mutation analysis of the mating-type locus in yeast.";
RL Cell 27:25-35(1981).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7021055; DOI=10.1101/sqb.1981.045.01.113;
RA Nasmyth K.A., Tatchell K., Hall B.D., Astell C., Smith M.;
RT "Physical analysis of mating-type loci in Saccharomyces cerevisiae.";
RL Cold Spring Harb. Symp. Quant. Biol. 45:961-981(1981).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
RX PubMed=6256656; DOI=10.1038/289244a0;
RA Nasmyth K.A., Tatchell K., Hall B.D., Astell C.R., Smith M.;
RT "A position effect in the control of transcription at yeast mating type
RT loci.";
RL Nature 289:244-250(1981).
CC -!- FUNCTION: Mating type proteins are sequence specific DNA-binding
CC proteins that act as master switches in yeast differentiation by
CC controlling gene expression in a cell type-specific fashion. Silenced
CC copy of ALPHA2 at HML.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: There are three genetic loci for mating type genes in
CC S.cerevisiae. MAT is the expression locus that determines the mating
CC type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and
CC HMR (containing HMRA1 and HMRA2) represent silenced repositories of
CC mating type information. The mating type is determined by the MAT
CC locus, which contains either a copy of HML or of HMR. Diploid cells are
CC usually heterozygous for the MAT locus.
CC -!- SIMILARITY: Belongs to the TALE/M-ATYP homeobox family. {ECO:0000305}.
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DR EMBL; V01315; CAA24625.1; -; Genomic_DNA.
DR EMBL; L00059; AAA34678.1; -; Genomic_DNA.
DR EMBL; X59720; CAA42400.1; -; Genomic_DNA.
DR EMBL; V01314; CAA24623.1; -; Genomic_DNA.
DR EMBL; BK006937; DAA07421.1; -; Genomic_DNA.
DR PIR; S19398; JFBYA2.
DR RefSeq; NP_009866.1; NM_001178708.1.
DR RefSeq; NP_009868.3; NM_001178753.1.
DR AlphaFoldDB; P0CY09; -.
DR SMR; P0CY09; -.
DR BioGRID; 30922; 7.
DR BioGRID; 31022; 22.
DR STRING; 4932.YCL067C; -.
DR PaxDb; P0CY09; -.
DR EnsemblFungi; YCL067C_mRNA; YCL067C; YCL067C.
DR EnsemblFungi; YCR039C_mRNA; YCR039C; YCR039C.
DR GeneID; 850292; -.
DR GeneID; 850406; -.
DR KEGG; sce:YCL067C; -.
DR KEGG; sce:YCR039C; -.
DR SGD; S000000572; HMLALPHA2.
DR VEuPathDB; FungiDB:YCL067C; -.
DR VEuPathDB; FungiDB:YCR039C; -.
DR eggNOG; KOG0773; Eukaryota.
DR GeneTree; ENSGT00940000176654; -.
DR HOGENOM; CLU_091806_1_0_1; -.
DR InParanoid; P0CY09; -.
DR OMA; KVQIKNW; -.
DR BioCyc; YEAST:G3O-29314-MON; -.
DR PRO; PR:P0CY09; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P0CY09; protein.
DR GO; GO:0005634; C:nucleus; ISA:SGD.
DR GO; GO:0008301; F:DNA binding, bending; ISA:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISA:SGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 3: Inferred from homology;
KW Acetylation; DNA-binding; Homeobox; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..210
FT /note="Silenced mating-type protein ALPHA2"
FT /id="PRO_0000410464"
FT DNA_BIND 129..191
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..102
FT /note="N-terminal domain"
FT REGION 103..128
FT /note="Flexible linker"
FT REGION 190..210
FT /note="C-terminal tail"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P0CY08"
FT CONFLICT 56
FT /note="G -> V (in Ref. 1; CAA24625, 2 and 3; AAA34678)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 210 AA; 24282 MW; 30CD6A486D7D76CE CRC64;
MNKIPIKDLL NPQITDEFKS SILDINKKLF SICCNLPKLP ESVTTEEEVE LRDILGFLSR
ANKNRKISDE EKKLLQTTSQ LTTTITVLLK EMRSIENDRS NYQLTQKNKS ADGLVFNVVT
QDMINKSTKP YRGHRFTKEN VRILESWFAK NIENPYLDTK GLENLMKNTS LSRIQIKNWV
SNRRRKEKTI TIAPELADLL SGEPLAKKKE
