HMCS1_BLAGE
ID HMCS1_BLAGE Reviewed; 453 AA.
AC P54961;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Hydroxymethylglutaryl-CoA synthase 1;
DE Short=HMG-CoA synthase 1;
DE EC=2.3.3.10;
DE AltName: Full=3-hydroxy-3-methylglutaryl coenzyme A synthase 1;
GN Name=HMGCS-1;
OS Blattella germanica (German cockroach) (Blatta germanica).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Polyneoptera; Dictyoptera; Blattodea; Blaberoidea; Ectobiidae;
OC Blattellinae; Blattella.
OX NCBI_TaxID=6973;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7901012; DOI=10.1111/j.1432-1033.1993.tb18295.x;
RA Martinez-Gonzalez J., Buesa C., Piulachs M.D., Belles X., Hegardt F.G.;
RT "3-hydroxy-3-methylglutaryl-coenzyme-A synthase from Blattella germanica.
RT Cloning, expression, developmental pattern and tissue expression.";
RL Eur. J. Biochem. 217:691-699(1993).
CC -!- FUNCTION: This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form
CC HMG-CoA, which is the substrate for HMG-CoA reductase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-hydroxy-3-
CC methylglutaryl-CoA + CoA + H(+); Xref=Rhea:RHEA:10188,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43074,
CC ChEBI:CHEBI:57286, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.10;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10116};
CC -!- PATHWAY: Metabolic intermediate biosynthesis; (R)-mevalonate
CC biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. HMG-CoA synthase
CC family. {ECO:0000305}.
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DR EMBL; X73679; CAA52032.1; -; mRNA.
DR PIR; S38986; S38986.
DR AlphaFoldDB; P54961; -.
DR SMR; P54961; -.
DR BRENDA; 2.3.3.10; 875.
DR UniPathway; UPA00058; UER00102.
DR GO; GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:InterPro.
DR GO; GO:0010142; P:farnesyl diphosphate biosynthetic process, mevalonate pathway; IEA:InterPro.
DR GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR000590; HMG_CoA_synt_AS.
DR InterPro; IPR013746; HMG_CoA_synt_C_dom.
DR InterPro; IPR013528; HMG_CoA_synth_N.
DR InterPro; IPR010122; HMG_CoA_synthase_euk.
DR InterPro; IPR016039; Thiolase-like.
DR Pfam; PF08540; HMG_CoA_synt_C; 1.
DR Pfam; PF01154; HMG_CoA_synt_N; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR TIGRFAMs; TIGR01833; HMG-CoA-S_euk; 1.
DR PROSITE; PS01226; HMG_COA_SYNTHASE; 1.
PE 2: Evidence at transcript level;
KW Lipid biosynthesis; Lipid metabolism; Steroid biosynthesis;
KW Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase.
FT CHAIN 1..453
FT /note="Hydroxymethylglutaryl-CoA synthase 1"
FT /id="PRO_0000213752"
FT ACT_SITE 84
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10116"
FT ACT_SITE 116
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10116"
FT ACT_SITE 246
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10116"
SQ SEQUENCE 453 AA; 50332 MW; C79EB2376270F348 CRC64;
MWPSDVGIVA LELIFPSQYV DQVDLEVYDN VSAGKYTVGL GQARMGFCTD REDINSLCLT
VVSRLMERWS IPYSQIGRLE VGTETLLDKS KSVKTVLMQL FKDNTDIEGV DTVNACYGGT
SALFNAISWV ESSSWDGRYA LVVAGDIAVY AKGSARPTGG AGAVAMLVGA NAPLVFDRGV
RSSHMQHAYD FYKPDLSSLY PTVDGKLSIQ CYLSALDHCY QLYCSKIQKQ LGEKFDIERL
DAVLFHAPYC KLVQKSLARL VLNDFVRASE EERTTKYSSL EALKGVKLED TYFDREVEKA
VMTYSKNMFE EKTKPSLLLA NQVGNMYTPS LYGGLVSLLV SKSAQELAGK RVALFSYGSG
LASSMFSLRI SSDASAKSSL QRLVSNLSHI KPQLDLRHKV SPEEFAQTME TREHNHHKAP
YTPEGSIDVL FPGTWYLESV DSLYRRSYKQ VPG
