HMCS2_BLAGE
ID HMCS2_BLAGE Reviewed; 455 AA.
AC P54870;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Hydroxymethylglutaryl-CoA synthase 2;
DE Short=HMG-CoA synthase 2;
DE EC=2.3.3.10;
DE AltName: Full=3-hydroxy-3-methylglutaryl coenzyme A synthase 2;
GN Name=HMGCS-2;
OS Blattella germanica (German cockroach) (Blatta germanica).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Polyneoptera; Dictyoptera; Blattodea; Blaberoidea; Ectobiidae;
OC Blattellinae; Blattella.
OX NCBI_TaxID=6973;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7909314; DOI=10.1016/s0021-9258(17)32630-3;
RA Buesa C., Martinez-Gonzalez J., Casals N., Haro D., Piulachs M.D.,
RA Belles X., Hegardt F.G.;
RT "Blattella germanica has two HMG-CoA synthase genes. Both are regulated in
RT the ovary during the gonadotrophic cycle.";
RL J. Biol. Chem. 269:11707-11713(1994).
CC -!- FUNCTION: This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form
CC HMG-CoA, which is the substrate for HMG-CoA reductase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-hydroxy-3-
CC methylglutaryl-CoA + CoA + H(+); Xref=Rhea:RHEA:10188,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43074,
CC ChEBI:CHEBI:57286, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.10;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10116};
CC -!- PATHWAY: Metabolic intermediate biosynthesis; (R)-mevalonate
CC biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. HMG-CoA synthase
CC family. {ECO:0000305}.
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DR EMBL; X77516; CAA54652.1; -; mRNA.
DR PIR; A53565; A53565.
DR AlphaFoldDB; P54870; -.
DR SMR; P54870; -.
DR BRENDA; 2.3.3.10; 875.
DR UniPathway; UPA00058; UER00102.
DR GO; GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:InterPro.
DR GO; GO:0010142; P:farnesyl diphosphate biosynthetic process, mevalonate pathway; IEA:InterPro.
DR GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR000590; HMG_CoA_synt_AS.
DR InterPro; IPR013746; HMG_CoA_synt_C_dom.
DR InterPro; IPR013528; HMG_CoA_synth_N.
DR InterPro; IPR010122; HMG_CoA_synthase_euk.
DR InterPro; IPR016039; Thiolase-like.
DR Pfam; PF08540; HMG_CoA_synt_C; 1.
DR Pfam; PF01154; HMG_CoA_synt_N; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR TIGRFAMs; TIGR01833; HMG-CoA-S_euk; 1.
DR PROSITE; PS01226; HMG_COA_SYNTHASE; 1.
PE 2: Evidence at transcript level;
KW Lipid biosynthesis; Lipid metabolism; Steroid biosynthesis;
KW Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase.
FT CHAIN 1..455
FT /note="Hydroxymethylglutaryl-CoA synthase 2"
FT /id="PRO_0000213753"
FT ACT_SITE 86
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10116"
FT ACT_SITE 120
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10116"
FT ACT_SITE 255
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10116"
SQ SEQUENCE 455 AA; 51425 MW; DD030356548A63B1 CRC64;
MAHWPEDVGI IGIEMIFPSL YVDQAELETY DEVSPGKYTM GLGQDKMGVC TDREDINSLC
LTAVDKLMER NNIDYNDIGW LEVGTETILD KVKSVKTVLM QLFEESGNTD VEGIDTINAC
YRGTAALFNA LIWIESSSWD GRYAIVVAAD IAIYAKECSP TGGAGALLML IGANAPIVID
RGVRASHMKH AYDFYKPDLM SEYPVVDGKL SVQCYLSALD HCYPRFCSKT EKYLKRCGKE
NTKIDLDYFD AFVFHSPYCK LVQKSVARLV LNDFIQYPEK YQDLQQLRNL KFEDTYFDRD
IEKIFMDKSK QLFEKKTKPS LMLANQVGNM YTTSLYGGLV SLLISEDIGE LAGKCICMFS
YGSGFAASMF SLHISTDSSP GSTLSRLVTN LTHIKPQVQQ RVKLSPGEFE NIMEIREQNH
HKAPYTPVAS PNTLFPGTWY LESIDSMHRR KYKRV
