HMCSB_DICDI
ID HMCSB_DICDI Reviewed; 468 AA.
AC Q86HL5; Q555J1;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Hydroxymethylglutaryl-CoA synthase B;
DE Short=HMG-CoA synthase B;
DE EC=2.3.3.10;
DE AltName: Full=3-hydroxy-3-methylglutaryl coenzyme A synthase B;
GN Name=hgsB; ORFNames=DDB_G0274871;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA,
CC which is the substrate for HMG-CoA reductase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-hydroxy-3-
CC methylglutaryl-CoA + CoA + H(+); Xref=Rhea:RHEA:10188,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43074,
CC ChEBI:CHEBI:57286, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.10;
CC -!- PATHWAY: Metabolic intermediate biosynthesis; (R)-mevalonate
CC biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. HMG-CoA synthase
CC family. {ECO:0000305}.
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DR EMBL; AAFI02000012; EAL70328.1; -; Genomic_DNA.
DR RefSeq; XP_644076.1; XM_638984.1.
DR AlphaFoldDB; Q86HL5; -.
DR SMR; Q86HL5; -.
DR STRING; 44689.DDB0217522; -.
DR PaxDb; Q86HL5; -.
DR EnsemblProtists; EAL70328; EAL70328; DDB_G0274871.
DR GeneID; 8619505; -.
DR KEGG; ddi:DDB_G0274871; -.
DR dictyBase; DDB_G0274871; hgsB.
DR eggNOG; KOG1393; Eukaryota.
DR HOGENOM; CLU_008065_0_1_1; -.
DR InParanoid; Q86HL5; -.
DR OMA; YFAFHAP; -.
DR PhylomeDB; Q86HL5; -.
DR Reactome; R-DDI-191273; Cholesterol biosynthesis.
DR Reactome; R-DDI-77111; Synthesis of Ketone Bodies.
DR UniPathway; UPA00058; UER00102.
DR PRO; PR:Q86HL5; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IBA:GO_Central.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IBA:GO_Central.
DR GO; GO:0010142; P:farnesyl diphosphate biosynthetic process, mevalonate pathway; IBA:GO_Central.
DR GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR013746; HMG_CoA_synt_C_dom.
DR InterPro; IPR013528; HMG_CoA_synth_N.
DR InterPro; IPR010122; HMG_CoA_synthase_euk.
DR InterPro; IPR016039; Thiolase-like.
DR Pfam; PF08540; HMG_CoA_synt_C; 1.
DR Pfam; PF01154; HMG_CoA_synt_N; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR TIGRFAMs; TIGR01833; HMG-CoA-S_euk; 1.
PE 3: Inferred from homology;
KW Lipid biosynthesis; Lipid metabolism; Reference proteome;
KW Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis;
KW Sterol metabolism; Transferase.
FT CHAIN 1..468
FT /note="Hydroxymethylglutaryl-CoA synthase B"
FT /id="PRO_0000340242"
FT ACT_SITE 85
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 119
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000250"
FT ACT_SITE 250
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 468 AA; 52921 MW; D44CCE967EF23BC6 CRC64;
MKKTKDIGIC AIDIYFPQTY VNQSELKKYD KVSNGKYTIG LGQTNMSFVG DREDIVSMAM
TSVKMMMSKY SIDYQSIGRL EVGTETIIDK SKSVKSSIMS LFQEYGNTSL EGVDTLNACY
GGTNALFNSL QWIESSYWDG RYALVVTGDI AVYSKGAARP TGGAGVVTML IGPNATLIFD
QSLRGTHMEN VNDFYKPDLS SEYPYVDGKL SIECYLRALD KCYLEYKKKF ESINDDNKFS
MDSFDYVCFH SPYNRLVQKS YARLIYNDFL QNPNNPKYQD LLPFKDLSTG KDSYINSKLD
QITLKLSLDD FKTKVNPSTL LSKECGNSYC GSVYSGILSL LSNVNDLNNK KVLVFSYGSG
LAASLFSFRI NNNKNRNNNN NNNNCFFKTT NDIGKISNIK ERLSNRVKVS PEEFTRILDI
REKSHQMVGA RTPIDTLDYI SAGTFYLEKI DEKLIRHYKS KPIISSKL
