HMDH1_ORYSI
ID HMDH1_ORYSI Reviewed; 539 AA.
AC A2X8W3; P48019;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 1;
DE Short=HMG-CoA reductase 1;
DE EC=1.1.1.34;
GN Name=HMG1; ORFNames=OsI_008506;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. IR36;
RX PubMed=8049366; DOI=10.1007/bf00043869;
RA Nelson A.J., Doerner P.W., Zhu Q., Lamb C.J.;
RT "Isolation of a monocot 3-hydroxy-3-methylglutaryl coenzyme A reductase
RT gene that is elicitor-inducible.";
RL Plant Mol. Biol. 25:401-412(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Catalyzes the synthesis of mevalonate. The specific precursor
CC of all isoprenoid compounds present in plants.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-mevalonate + CoA + 2 NADP(+) = (3S)-hydroxy-3-
CC methylglutaryl-CoA + 2 H(+) + 2 NADPH; Xref=Rhea:RHEA:15989,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:36464, ChEBI:CHEBI:43074,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.34;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10003};
CC -!- PATHWAY: Metabolic intermediate biosynthesis; (R)-mevalonate
CC biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein.
CC -!- SIMILARITY: Belongs to the HMG-CoA reductase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA21720.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; L28995; AAA21720.1; ALT_FRAME; Genomic_DNA.
DR EMBL; CM000127; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S46314; S46314.
DR AlphaFoldDB; A2X8W3; -.
DR SMR; A2X8W3; -.
DR STRING; 39946.A2X8W3; -.
DR EnsemblPlants; BGIOSGA005742-TA; BGIOSGA005742-PA; BGIOSGA005742.
DR Gramene; BGIOSGA005742-TA; BGIOSGA005742-PA; BGIOSGA005742.
DR HOGENOM; CLU_001734_2_2_1; -.
DR OMA; DDKMTRA; -.
DR UniPathway; UPA00058; UER00103.
DR Proteomes; UP000007015; Chromosome 2.
DR ExpressionAtlas; A2X8W3; differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH) activity; IEA:UniProtKB-EC.
DR GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd00643; HMG-CoA_reductase_classI; 1.
DR Gene3D; 1.10.3270.10; -; 1.
DR Gene3D; 3.30.70.420; -; 1.
DR Gene3D; 3.90.770.10; -; 1.
DR InterPro; IPR002202; HMG_CoA_Rdtase.
DR InterPro; IPR023074; HMG_CoA_Rdtase_cat_sf.
DR InterPro; IPR023076; HMG_CoA_Rdtase_CS.
DR InterPro; IPR004554; HMG_CoA_Rdtase_eu_arc.
DR InterPro; IPR023282; HMG_CoA_Rdtase_N.
DR InterPro; IPR009023; HMG_CoA_Rdtase_NAD(P)-bd_sf.
DR InterPro; IPR009029; HMG_CoA_Rdtase_sub-bd_dom_sf.
DR PANTHER; PTHR10572; PTHR10572; 1.
DR Pfam; PF00368; HMG-CoA_red; 1.
DR PRINTS; PR00071; HMGCOARDTASE.
DR SUPFAM; SSF55035; SSF55035; 1.
DR SUPFAM; SSF56542; SSF56542; 1.
DR TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR PROSITE; PS01192; HMG_COA_REDUCTASE_3; 1.
DR PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane; NADP;
KW Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..539
FT /note="3-hydroxy-3-methylglutaryl-coenzyme A reductase 1"
FT /id="PRO_0000295657"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 496..516
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 84..124
FT /note="Linker"
FT /evidence="ECO:0000250"
FT REGION 87..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 125..539
FT /note="Catalytic"
FT /evidence="ECO:0000250"
FT ACT_SITE 218
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 350
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 426
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 524
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10003"
FT CARBOHYD 282
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 528
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 48
FT /note="L -> V (in Ref. 1; AAA21720)"
FT /evidence="ECO:0000305"
FT CONFLICT 104..108
FT /note="RPAAA -> APP (in Ref. 1; AAA21720)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="A -> G (in Ref. 1; AAA21720)"
FT /evidence="ECO:0000305"
FT CONFLICT 257..258
FT /note="SA -> CP (in Ref. 1; AAA21720)"
FT /evidence="ECO:0000305"
FT CONFLICT 444
FT /note="D -> N (in Ref. 1; AAA21720)"
FT /evidence="ECO:0000305"
FT CONFLICT 494
FT /note="K -> G (in Ref. 1; AAA21720)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 539 AA; 56331 MW; 031F08C2671B910E CRC64;
MDVRRGGGGG RIVGAARRAL TWGALPLPMR ITNGLAMVSL VLSSCDLLRL CSDRERPLGG
REFATVVCQL ASVVYLLSLF AHPDAPATTT GDDDDGQGGS RRARPAAAEP APMHGHGGGM
MEADDEEIVA AVASGALPSH RLESRLGDCR RAARLRREAL RRVTGRGVEG LPFDGMDYQA
ILGQCCEMPV GYVQLPVGVA GPLLLDGREY HVPMATTEGC LVASVNRGCR AISASGGAFS
VLLRDAMSRA PAVKLPSAMR AAELKAFAEA PANFELLAAV FNRSSRFGRL QDIRCALAGR
NLYMRFSCIT GDAMGMNMVS KGVENVLGYL QNVFPDMDVI SVSGNYCSDK KPTAVNWIEG
RGKSVVCEAI IKGDVVQKVL KTTVEKLVEL NIIKNLAGSA VAGALGGFNA HASNIVTALF
IATGQDPAQN VESSQCITML EEVDDGDDLH ISVTMPSIEV GTIGGGTCLA SQAACLNLLG
VKGSNHGSPG ANAKRLATIV AGSVLAGELS LLAALASGHL VKSHMMYNRS SKDVAKAAS
