HMDH2_SOLLC
ID HMDH2_SOLLC Reviewed; 602 AA.
AC P48022; O24028;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 2;
DE Short=HMG-CoA reductase 2;
DE EC=1.1.1.34;
GN Name=HMG2;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. VFNT Cherry;
RX PubMed=1391777; DOI=10.1007/bf00014502;
RA Park H.S., Denbow C.J., Cramer C.L.;
RT "Structure and nucleotide sequence of tomato HMG2 encoding 3-hydroxy-3-
RT methyl-glutaryl coenzyme A reductase.";
RL Plant Mol. Biol. 20:327-331(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8883384; DOI=10.1104/pp.112.2.727;
RA Daraselia N.D., Tarchevskaya S., Narita J.O.;
RT "The promoter for tomato 3-hydroxy-3-methylglutaryl coenzyme A reductase
RT gene 2 has unusual regulatory elements that direct high-level expression.";
RL Plant Physiol. 112:727-733(1996).
CC -!- FUNCTION: Catalyzes the synthesis of mevalonate. The specific precursor
CC of all isoprenoid compounds present in plants.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-mevalonate + CoA + 2 NADP(+) = (3S)-hydroxy-3-
CC methylglutaryl-CoA + 2 H(+) + 2 NADPH; Xref=Rhea:RHEA:15989,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:36464, ChEBI:CHEBI:43074,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.34;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10003};
CC -!- PATHWAY: Metabolic intermediate biosynthesis; (R)-mevalonate
CC biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein.
CC -!- SIMILARITY: Belongs to the HMG-CoA reductase family. {ECO:0000305}.
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DR EMBL; M63642; AAA34169.1; -; Genomic_DNA.
DR EMBL; U68072; AAB62581.1; -; Genomic_DNA.
DR PIR; S25316; S25316.
DR RefSeq; NP_001296119.1; NM_001309190.1.
DR AlphaFoldDB; P48022; -.
DR SMR; P48022; -.
DR STRING; 4081.Solyc02g038740.2.1; -.
DR PaxDb; P48022; -.
DR PRIDE; P48022; -.
DR GeneID; 101265292; -.
DR KEGG; sly:101265292; -.
DR eggNOG; KOG2480; Eukaryota.
DR InParanoid; P48022; -.
DR OrthoDB; 907394at2759; -.
DR UniPathway; UPA00058; UER00103.
DR Proteomes; UP000004994; Unplaced.
DR ExpressionAtlas; P48022; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005778; C:peroxisomal membrane; IBA:GO_Central.
DR GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH) activity; IBA:GO_Central.
DR GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR GO; GO:0008299; P:isoprenoid biosynthetic process; IBA:GO_Central.
DR GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR CDD; cd00643; HMG-CoA_reductase_classI; 1.
DR Gene3D; 1.10.3270.10; -; 1.
DR Gene3D; 3.30.70.420; -; 1.
DR Gene3D; 3.90.770.10; -; 1.
DR InterPro; IPR002202; HMG_CoA_Rdtase.
DR InterPro; IPR023074; HMG_CoA_Rdtase_cat_sf.
DR InterPro; IPR023076; HMG_CoA_Rdtase_CS.
DR InterPro; IPR004554; HMG_CoA_Rdtase_eu_arc.
DR InterPro; IPR023282; HMG_CoA_Rdtase_N.
DR InterPro; IPR009023; HMG_CoA_Rdtase_NAD(P)-bd_sf.
DR InterPro; IPR009029; HMG_CoA_Rdtase_sub-bd_dom_sf.
DR PANTHER; PTHR10572; PTHR10572; 1.
DR Pfam; PF00368; HMG-CoA_red; 1.
DR PRINTS; PR00071; HMGCOARDTASE.
DR SUPFAM; SSF55035; SSF55035; 1.
DR SUPFAM; SSF56542; SSF56542; 1.
DR TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR PROSITE; PS01192; HMG_COA_REDUCTASE_3; 1.
DR PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane; NADP;
KW Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..602
FT /note="3-hydroxy-3-methylglutaryl-coenzyme A reductase 2"
FT /id="PRO_0000114443"
FT TRANSMEM 44..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 116..187
FT /note="Linker"
FT /evidence="ECO:0000250"
FT REGION 188..602
FT /note="Catalytic"
FT /evidence="ECO:0000250"
FT ACT_SITE 281
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 413
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 489
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 587
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10003"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 345
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 458
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 591
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 56
FT /note="Missing (in Ref. 2; AAB62581)"
FT /evidence="ECO:0000305"
FT CONFLICT 186
FT /note="G -> E (in Ref. 2; AAB62581)"
FT /evidence="ECO:0000305"
FT CONFLICT 339
FT /note="S -> T (in Ref. 2; AAB62581)"
FT /evidence="ECO:0000305"
FT CONFLICT 369..370
FT /note="LC -> FV (in Ref. 2; AAB62581)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 602 AA; 64715 MW; 0266E55E2EB953BD CRC64;
MDVRRRSEEP VYPSKVFAAD EKPLKPHKKQ QQQQEDKNTL LIDASDALPL PLYLTTNGLF
FTMFFSVMYF LLSRWREKIR NSTPLHVVTL SELGAIVSLI ASVIYLLGFF GIGFVQTFVS
RGNNDSWDEN DEEFLLKEDS RCGPATTLGC AVPAPPARQI APMAPPQPSM SMVEKPAPLI
TSASSGEDEE IIKSVVQGKI PSYSLESKLG DCKRAASIRK EVMQRITGKS LEGLPLEGFN
YESILGQCCE MPIGYVQIPV GIAGPLLLNG KEFSVPMATT EGCLVASTNR GCKAIYASGG
ATCILLRDGM TRAPCVRFGT AKRAAELKFF VEDPIKFESL ANVFNQSSRF ARLQRIQCAI
AGKNLYMRLC CSTGDAMGMN MVSKGVQNVL DYLQNEYPDM DVIGISGNFC SDKKPAAVNW
IEGRGKSVVC EAIITEEVVK KVLKTEVAAL VELNMLKNLT GSAMAGALGG FNAHASNIVS
AVFIATGQDP AQNIESSHCI TMMEAVNDGK DLHISVTMPS IEVGTVGGGT QLASQSACLN
LLGVKGANRE APGSNARLLA TVVAGSVLAG ELSLMSAISS GQLVNSHMKY NRSTKDVTKA
SS
