HMDH_MAIZE
ID HMDH_MAIZE Reviewed; 579 AA.
AC O24594;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase;
DE Short=HMG-CoA reductase;
DE EC=1.1.1.34;
GN Name=HMGR;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. LG 21-11;
RA Mayer M.J., Steel J., Bailey J.A., Hargreaves J.A.;
RL Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the synthesis of mevalonate. The specific precursor
CC of all isoprenoid compounds present in plants.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-mevalonate + CoA + 2 NADP(+) = (3S)-hydroxy-3-
CC methylglutaryl-CoA + 2 H(+) + 2 NADPH; Xref=Rhea:RHEA:15989,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:36464, ChEBI:CHEBI:43074,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.34;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10003};
CC -!- PATHWAY: Metabolic intermediate biosynthesis; (R)-mevalonate
CC biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein.
CC -!- SIMILARITY: Belongs to the HMG-CoA reductase family. {ECO:0000305}.
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DR EMBL; Y09238; CAA70440.1; -; Genomic_DNA.
DR PIR; T04357; T04357.
DR AlphaFoldDB; O24594; -.
DR SMR; O24594; -.
DR STRING; 4577.GRMZM2G393337_P02; -.
DR PaxDb; O24594; -.
DR PRIDE; O24594; -.
DR eggNOG; KOG2480; Eukaryota.
DR UniPathway; UPA00058; UER00103.
DR Proteomes; UP000007305; Unplaced.
DR ExpressionAtlas; O24594; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005778; C:peroxisomal membrane; IBA:GO_Central.
DR GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH) activity; IBA:GO_Central.
DR GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR GO; GO:0008299; P:isoprenoid biosynthetic process; IBA:GO_Central.
DR GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR CDD; cd00643; HMG-CoA_reductase_classI; 1.
DR Gene3D; 1.10.3270.10; -; 1.
DR Gene3D; 3.30.70.420; -; 1.
DR Gene3D; 3.90.770.10; -; 1.
DR InterPro; IPR002202; HMG_CoA_Rdtase.
DR InterPro; IPR023074; HMG_CoA_Rdtase_cat_sf.
DR InterPro; IPR023076; HMG_CoA_Rdtase_CS.
DR InterPro; IPR004554; HMG_CoA_Rdtase_eu_arc.
DR InterPro; IPR023282; HMG_CoA_Rdtase_N.
DR InterPro; IPR009023; HMG_CoA_Rdtase_NAD(P)-bd_sf.
DR InterPro; IPR009029; HMG_CoA_Rdtase_sub-bd_dom_sf.
DR PANTHER; PTHR10572; PTHR10572; 1.
DR Pfam; PF00368; HMG-CoA_red; 1.
DR PRINTS; PR00071; HMGCOARDTASE.
DR SUPFAM; SSF55035; SSF55035; 1.
DR SUPFAM; SSF56542; SSF56542; 1.
DR TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR PROSITE; PS01192; HMG_COA_REDUCTASE_3; 1.
DR PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane; NADP;
KW Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..579
FT /note="3-hydroxy-3-methylglutaryl-coenzyme A reductase"
FT /id="PRO_0000114444"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 101..153
FT /note="Linker"
FT /evidence="ECO:0000250"
FT REGION 154..579
FT /note="Catalytic"
FT /evidence="ECO:0000250"
FT REGION 555..579
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 247
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 379
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 455
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 553
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10003"
FT CARBOHYD 311
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 557
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 579 AA; 60931 MW; ECA693F232EB2B6A CRC64;
MEVRGGVGQG SAARHPPAPE PSRAAARVQA GDALPLPIRH TNLIFSALFA ASLAYLMRRW
REKIRSSTPL HAVGLAEMLA IFGLVASLIY LLSFFGIAFV QSIVSSGDDD EDFLVGSGSS
GSAAAPSRQH AQAPAPCELL GSPAAAPEKM PEDDEEIVAS VVAGKVPSYA LEARLGDCRR
AAGIRREALR RITGRDIEGL PLDGFDYASI LGQCCELPVG YVQLPVGVAG PLLLDGRRFY
LPMATTEGCL VASTNRGCKA IAESGGATSV VLRDAMTRAP VARFPTARRA AELKAFLEDP
ANFDTLSVVF NRSSRFARLQ GVQCAMAGRN LYMRFSCSTG DAMGMNMVSK GVQNVLDFLQ
DDFHDMDVIS ISGNFCSDKK PSAVNWIEGR GKSVVCEAVI GEEVVKKVLK TDVQSLVELN
TIKNLAGSAV AGALGGFNAH ASNIVTAIFI ATGQDPAQNV ESSHCITMLE PVNAGRDLHI
SVTMPSIEVG TVGGGTQLAS QSACLDLLGV RGASRDRPGS NARLLATVVA GGVLAGELSL
LSALAAGQLV KSHMKYNRSS KDVSSTTATE KTRQREVDV
