HMDH_PYRAB
ID HMDH_PYRAB Reviewed; 408 AA.
AC Q9V1R3; G8ZHZ8;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2012, sequence version 2.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase;
DE Short=HMG-CoA reductase;
DE EC=1.1.1.34;
GN Name=hmgA; OrderedLocusNames=PYRAB03640; ORFNames=PAB2106;
OS Pyrococcus abyssi (strain GE5 / Orsay).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=272844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay;
RX PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic archaeon
RT Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=GE5 / Orsay;
RX PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA Gao J., Wang J.;
RT "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT Pyrococcus furiosus DSM 3638.";
RL Curr. Microbiol. 64:118-129(2012).
CC -!- FUNCTION: Converts HMG-CoA to mevalonate. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-mevalonate + CoA + 2 NADP(+) = (3S)-hydroxy-3-
CC methylglutaryl-CoA + 2 H(+) + 2 NADPH; Xref=Rhea:RHEA:15989,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:36464, ChEBI:CHEBI:43074,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.34;
CC -!- PATHWAY: Metabolic intermediate biosynthesis; (R)-mevalonate
CC biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
CC -!- SIMILARITY: Belongs to the HMG-CoA reductase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB49286.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ248284; CAB49286.1; ALT_INIT; Genomic_DNA.
DR EMBL; HE613800; CCE69741.1; -; Genomic_DNA.
DR PIR; G75150; G75150.
DR RefSeq; WP_048146539.1; NC_000868.1.
DR AlphaFoldDB; Q9V1R3; -.
DR SMR; Q9V1R3; -.
DR STRING; 272844.PAB2106; -.
DR EnsemblBacteria; CAB49286; CAB49286; PAB2106.
DR GeneID; 1495254; -.
DR KEGG; pab:PAB2106; -.
DR PATRIC; fig|272844.11.peg.385; -.
DR eggNOG; arCOG04260; Archaea.
DR HOGENOM; CLU_001734_2_2_2; -.
DR OrthoDB; 26445at2157; -.
DR UniPathway; UPA00058; UER00103.
DR Proteomes; UP000000810; Chromosome.
DR Proteomes; UP000009139; Chromosome.
DR GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH) activity; IEA:UniProtKB-EC.
DR GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd00643; HMG-CoA_reductase_classI; 1.
DR Gene3D; 1.10.3270.10; -; 1.
DR Gene3D; 3.30.70.420; -; 1.
DR Gene3D; 3.90.770.10; -; 1.
DR InterPro; IPR002202; HMG_CoA_Rdtase.
DR InterPro; IPR023074; HMG_CoA_Rdtase_cat_sf.
DR InterPro; IPR023076; HMG_CoA_Rdtase_CS.
DR InterPro; IPR004554; HMG_CoA_Rdtase_eu_arc.
DR InterPro; IPR023282; HMG_CoA_Rdtase_N.
DR InterPro; IPR009023; HMG_CoA_Rdtase_NAD(P)-bd_sf.
DR InterPro; IPR009029; HMG_CoA_Rdtase_sub-bd_dom_sf.
DR PANTHER; PTHR10572; PTHR10572; 1.
DR Pfam; PF00368; HMG-CoA_red; 1.
DR PRINTS; PR00071; HMGCOARDTASE.
DR SUPFAM; SSF55035; SSF55035; 1.
DR SUPFAM; SSF56542; SSF56542; 1.
DR TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE 3: Inferred from homology;
KW Isoprene biosynthesis; NADP; Oxidoreductase.
FT CHAIN 1..408
FT /note="3-hydroxy-3-methylglutaryl-coenzyme A reductase"
FT /id="PRO_0000114463"
FT ACT_SITE 101
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 307
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 403
FT /note="Proton donor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 408 AA; 44007 MW; 9F8BD5BDF1ABA97B CRC64;
MNVEDIIEKV ANGEIKLHQV EKYVNGDKRL ATEIRRKALE RKLGISLKHI GHYSIDPNEL
IGRNIENMIG VVQIPMGVAG PLKINGEYAK GEFYIPLATT EGALVASVNR GCSALTEAGG
VVTTILDDKM TRAPLIRCPN ARRAREVAEW VKENLNYLQE KAVAKVTRHG KLRDVKPFIV
GNNLYLRFEF ETGDAMGMNM VTIASEEIMK VIEEEFPDVR YLALSGNLCV DKKPNAVNFI
LGRGKTVVAE AIVPREIVEK KLKTTPELIA EVNYFKNLVG SAQAGSYGFN AHFGNIVGAI
FLATGQDEAQ ITEGSHGITI AEVTPEGDLY ISITMPSLEI GTVGGGTRVP TQREALSIMG
VAGGGDPPGV NAKKFAEIVA GAVLAGELSL LAAIAAKHLA RAHKMLGR
