HMD_METJA
ID HMD_METJA Reviewed; 358 AA.
AC Q58194;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=5,10-methenyltetrahydromethanopterin hydrogenase;
DE EC=1.12.98.2;
DE AltName: Full=H(2)-dependent methylene-H(4)MPT dehydrogenase;
DE AltName: Full=H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase;
DE AltName: Full=N(5),N(10)-methenyltetrahydromethanopterin hydrogenase;
GN Name=hmd; OrderedLocusNames=MJ0784;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC methylene-H(4)MPT. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-
CC methylenetetrahydromethanopterin + H(+); Xref=Rhea:RHEA:20017,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:18276, ChEBI:CHEBI:57818,
CC ChEBI:CHEBI:58337; EC=1.12.98.2;
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC tetrahydromethanopterin (hydrogen route): step 1/1.
CC -!- SIMILARITY: Belongs to the HMD family. {ECO:0000305}.
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DR EMBL; L77117; AAB98781.1; -; Genomic_DNA.
DR PIR; H64397; H64397.
DR RefSeq; WP_010870290.1; NC_000909.1.
DR PDB; 2B0J; X-ray; 1.75 A; A=1-358.
DR PDB; 3DAF; X-ray; 1.75 A; A=1-358.
DR PDB; 3DAG; X-ray; 1.75 A; A=1-358.
DR PDB; 3F46; X-ray; 1.95 A; A=1-358.
DR PDB; 3F47; X-ray; 1.75 A; A=1-358.
DR PDB; 3H65; X-ray; 2.15 A; A=1-358.
DR PDBsum; 2B0J; -.
DR PDBsum; 3DAF; -.
DR PDBsum; 3DAG; -.
DR PDBsum; 3F46; -.
DR PDBsum; 3F47; -.
DR PDBsum; 3H65; -.
DR AlphaFoldDB; Q58194; -.
DR SMR; Q58194; -.
DR STRING; 243232.MJ_0784; -.
DR EnsemblBacteria; AAB98781; AAB98781; MJ_0784.
DR GeneID; 1451662; -.
DR KEGG; mja:MJ_0784; -.
DR eggNOG; arCOG03196; Archaea.
DR HOGENOM; CLU_772960_0_0_2; -.
DR OMA; HACTIPT; -.
DR OrthoDB; 31540at2157; -.
DR PhylomeDB; Q58194; -.
DR BRENDA; 1.12.98.2; 3260.
DR UniPathway; UPA00640; UER00696.
DR EvolutionaryTrace; Q58194; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0047068; F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.120.1300; -; 1.
DR HAMAP; MF_01090; HMD; 1.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR010062; HMD.
DR InterPro; IPR004889; HMD_C.
DR InterPro; IPR038182; HMD_C_sf.
DR InterPro; IPR024190; METHMP_Hmd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF03201; HMD; 1.
DR PIRSF; PIRSF016158; HMD; 1.
DR PIRSF; PIRSF500165; HMDI; 1.
DR SUPFAM; SSF48179; SSF48179; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR01723; hmd_TIGR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Methanogenesis; One-carbon metabolism; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..358
FT /note="5,10-methenyltetrahydromethanopterin hydrogenase"
FT /id="PRO_0000218508"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 12..17
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 23..32
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 35..39
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 42..53
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 59..63
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 65..68
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 69..75
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 81..89
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 93..95
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 97..109
FT /evidence="ECO:0007829|PDB:2B0J"
FT TURN 114..116
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 117..122
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 124..127
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 130..133
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 135..139
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 143..147
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 151..154
FT /evidence="ECO:0007829|PDB:3DAF"
FT HELIX 155..162
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 163..165
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 171..174
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 176..178
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 180..189
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 195..199
FT /evidence="ECO:0007829|PDB:2B0J"
FT TURN 206..208
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 212..219
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 221..235
FT /evidence="ECO:0007829|PDB:2B0J"
FT STRAND 238..242
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 243..245
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 246..250
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 254..272
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 279..300
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 302..304
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 305..308
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 311..316
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 318..321
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 324..326
FT /evidence="ECO:0007829|PDB:2B0J"
FT HELIX 329..339
FT /evidence="ECO:0007829|PDB:2B0J"
SQ SEQUENCE 358 AA; 38689 MW; 9D8E789759BCF43A CRC64;
MKIAILGAGC YRTHAAAGIT NFMRACEVAK EVGKPEIALT HSSITYGAEL LHLVPDVKEV
IVSDPCFAEE PGLVVIDEFD PKEVMEAHLS GNPESIMPKI REVVKAKAKE LPKPPKACIH
LVHPEDVGLK VTSDDREAVE GADIVITWLP KGNKQPDIIK KFADAIPEGA IVTHACTIPT
TKFAKIFKDL GREDLNITSY HPGCVPEMKG QVYIAEGYAS EEAVNKLYEI GKIARGKAFK
MPANLIGPVC DMCSAVTATV YAGLLAYRDA VTKILGAPAD FAQMMADEAL TQIHNLMKEK
GIANMEEALD PAALLGTADS MCFGPLAEIL PTALKVLEKH KVVEEEGKTK CEIMSQKE
