ID   HMD_METTF               Reviewed;         344 AA.
AC   Q50758;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=5,10-methenyltetrahydromethanopterin hydrogenase;
DE            EC=1.12.98.2;
DE   AltName: Full=H(2)-dependent methylene-H(4)MPT dehydrogenase;
DE   AltName: Full=H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase;
DE   AltName: Full=N(5),N(10)-methenyltetrahydromethanopterin hydrogenase;
GN   Name=hmd;
OS   Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=145262;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 43574 / DSM 3664 / OCM 36 / CB12;
RA   Vaupel M.;
RL   Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC       methylene-H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-
CC         methylenetetrahydromethanopterin + H(+); Xref=Rhea:RHEA:20017,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18276, ChEBI:CHEBI:57818,
CC         ChEBI:CHEBI:58337; EC=1.12.98.2;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC       methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC       tetrahydromethanopterin (hydrogen route): step 1/1.
CC   -!- SIMILARITY: Belongs to the HMD family. {ECO:0000305}.
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DR   EMBL; X92711; CAA63376.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q50758; -.
DR   SMR; Q50758; -.
DR   UniPathway; UPA00640; UER00696.
DR   GO; GO:0047068; F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.1300; -; 1.
DR   HAMAP; MF_01090; HMD; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR010062; HMD.
DR   InterPro; IPR004889; HMD_C.
DR   InterPro; IPR038182; HMD_C_sf.
DR   InterPro; IPR024190; METHMP_Hmd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF03201; HMD; 1.
DR   PIRSF; PIRSF016158; HMD; 1.
DR   PIRSF; PIRSF500165; HMDI; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01723; hmd_TIGR; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; One-carbon metabolism; Oxidoreductase.
FT   CHAIN           1..344
FT                   /note="5,10-methenyltetrahydromethanopterin hydrogenase"
FT                   /id="PRO_0000218510"
SQ   SEQUENCE   344 AA;  37525 MW;  33C6E5C0D5C030B7 CRC64;
     MKLAILGAGC YRTHAASGIT NFSRACEVAE MVGKPEIAMT HSTITMGAEL KELAGVDEVV
     VADPVFDNQF TVIDDFAYED VIEAHKEDPE KIMPQIREKV NEVAKELPKP PEGAIHFTHP
     EDLGFEITTD DREAVADADF IMTWFPKGDM QPGIINKFID DIKPGAIVTH ACTIPTTKFY
     KIFEEKHGDL VTRPETLNVT SYHPGAVPEM KGQVYIAEGY ASEEAINTLF ELGQKARGNA
     YKLPAELLGP VCDMCSALTA ITYAGILTYR DSVTQVLGAP AGFAQMMAKE SLEQLTALMD
     KVGIDKMEES LDPGALLGTA DSMNFGASAE ILPTVFEVLE KRKK