ID   HMD_METTH               Reviewed;         344 AA.
AC   O27211; Q50512;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=5,10-methenyltetrahydromethanopterin hydrogenase;
DE            EC=1.12.98.2;
DE   AltName: Full=H(2)-dependent methylene-H(4)MPT dehydrogenase;
DE   AltName: Full=H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase;
DE   AltName: Full=N(5),N(10)-methenyltetrahydromethanopterin hydrogenase;
GN   Name=hmd; Synonyms=mth; OrderedLocusNames=MTH_1142;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=7730278; DOI=10.1128/jb.177.9.2460-2468.1995;
RA   Noelling J., Pihl T.D., Vriesema A., Reeve J.N.;
RT   "Organization and growth phase-dependent transcription of methane genes in
RT   two regions of the Methanobacterium thermoautotrophicum genome.";
RL   J. Bacteriol. 177:2460-2468(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC       methylene-H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-
CC         methylenetetrahydromethanopterin + H(+); Xref=Rhea:RHEA:20017,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18276, ChEBI:CHEBI:57818,
CC         ChEBI:CHEBI:58337; EC=1.12.98.2;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC       methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC       tetrahydromethanopterin (hydrogen route): step 1/1.
CC   -!- SIMILARITY: Belongs to the HMD family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB85631.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U19363; AAA87423.1; -; Genomic_DNA.
DR   EMBL; AE000666; AAB85631.1; ALT_INIT; Genomic_DNA.
DR   PIR; B69019; B69019.
DR   RefSeq; WP_048060977.1; NC_000916.1.
DR   AlphaFoldDB; O27211; -.
DR   SMR; O27211; -.
DR   STRING; 187420.MTH_1142; -.
DR   PRIDE; O27211; -.
DR   EnsemblBacteria; AAB85631; AAB85631; MTH_1142.
DR   GeneID; 1471550; -.
DR   KEGG; mth:MTH_1142; -.
DR   PATRIC; fig|187420.15.peg.1119; -.
DR   HOGENOM; CLU_772960_0_0_2; -.
DR   OMA; HACTIPT; -.
DR   BioCyc; MetaCyc:HMDMAUTO-MON; -.
DR   UniPathway; UPA00640; UER00696.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0047068; F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.1300; -; 1.
DR   HAMAP; MF_01090; HMD; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR010062; HMD.
DR   InterPro; IPR004889; HMD_C.
DR   InterPro; IPR038182; HMD_C_sf.
DR   InterPro; IPR024190; METHMP_Hmd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF03201; HMD; 1.
DR   PIRSF; PIRSF016158; HMD; 1.
DR   PIRSF; PIRSF500165; HMDI; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01723; hmd_TIGR; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; One-carbon metabolism; Oxidoreductase; Reference proteome.
FT   CHAIN           1..344
FT                   /note="5,10-methenyltetrahydromethanopterin hydrogenase"
FT                   /id="PRO_0000218511"
SQ   SEQUENCE   344 AA;  37576 MW;  12E600166BB33E48 CRC64;
     MKLAILGAGC YRTHAASGIT NFSRACEVAE MVGKPEIAMT HSTITMGAEL KELAGVDEVV
     VADPVFDNQF TVIDDFAYED VIEAHKEDPE KIMPQIREKV NEVAKELPKP PEGAIHFTHP
     EDLGFEITTD DREAVADADF IMTWFPKGDM QPGIIDKFID DIKPGAIVTH ACTIPTTKFY
     KIFEEKHGDL VTKPETLNVT SYHPGAVPEM KGQVYIAEGY ASEEAINTLF ELGQKARGNA
     YRLPAELLGP VCDMCSALTA ITYAGILSYR DSVTQVLGAP AGFAQMMAKE SLEQITALME
     KVGIDKMEEH LDPGALLGTA DSMNFGASAD ILPTVFEILE KRKK