ID   HMD_METTW               Reviewed;         344 AA.
AC   P81221; Q50526;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=5,10-methenyltetrahydromethanopterin hydrogenase;
DE            EC=1.12.98.2;
DE   AltName: Full=H(2)-dependent methylene-H(4)MPT dehydrogenase;
DE   AltName: Full=H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase;
DE   AltName: Full=N(5),N(10)-methenyltetrahydromethanopterin hydrogenase;
GN   Name=hmd; Synonyms=mth;
OS   Methanothermobacter thermautotrophicus (strain Winter) (Methanobacterium
OS   thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=79930;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7730278; DOI=10.1128/jb.177.9.2460-2468.1995;
RA   Noelling J., Pihl T.D., Vriesema A., Reeve J.N.;
RT   "Organization and growth phase-dependent transcription of methane genes in
RT   two regions of the Methanobacterium thermoautotrophicum genome.";
RL   J. Bacteriol. 177:2460-2468(1995).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC       methylene-H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-
CC         methylenetetrahydromethanopterin + H(+); Xref=Rhea:RHEA:20017,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18276, ChEBI:CHEBI:57818,
CC         ChEBI:CHEBI:58337; EC=1.12.98.2;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC       methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC       tetrahydromethanopterin (hydrogen route): step 1/1.
CC   -!- SIMILARITY: Belongs to the HMD family. {ECO:0000305}.
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DR   EMBL; U19364; AAA87437.1; -; Genomic_DNA.
DR   AlphaFoldDB; P81221; -.
DR   SMR; P81221; -.
DR   UniPathway; UPA00640; UER00696.
DR   GO; GO:0047068; F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.1300; -; 1.
DR   HAMAP; MF_01090; HMD; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR010062; HMD.
DR   InterPro; IPR004889; HMD_C.
DR   InterPro; IPR038182; HMD_C_sf.
DR   InterPro; IPR024190; METHMP_Hmd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF03201; HMD; 1.
DR   PIRSF; PIRSF016158; HMD; 1.
DR   PIRSF; PIRSF500165; HMDI; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01723; hmd_TIGR; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; One-carbon metabolism; Oxidoreductase.
FT   CHAIN           1..344
FT                   /note="5,10-methenyltetrahydromethanopterin hydrogenase"
FT                   /id="PRO_0000218513"
SQ   SEQUENCE   344 AA;  37539 MW;  33C6E5C575C390B1 CRC64;
     MKLAILGAGC YRTHAASGIT NFSRACEVAE MVGKPEIAMT HSTITMGAEL KELAGVDEVV
     VADPVFDNQF TVIDDFAYED VIEAHKEDPE KIMPQIREKV NEVAKELPKP PEGAIHFTHP
     EDLGFEITTD DREAVADADF IMTWFPKGDM QPGIINKFID DIKPGAIVTH ACTIPTTKFY
     KIFEEKHGDL VTRPETLNVT SYHPGAVPEM KGQVYIAEAY ASEEAINTLF ELGQKARGNA
     YKLPAELLGP VCDMCSALTA ITYAGILTYR DSVTQVLGAP AGFAQMMAKE SLEQLTALMD
     KVGIDKMEES LDPGALLGTA DSMNFGASAE ILPTVFEVLE KRKK