ID   HMD_METVO               Reviewed;         343 AA.
AC   Q50840;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=5,10-methenyltetrahydromethanopterin hydrogenase;
DE            EC=1.12.98.2;
DE   AltName: Full=H(2)-dependent methylene-H(4)MPT dehydrogenase;
DE   AltName: Full=H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase;
DE   AltName: Full=N(5),N(10)-methenyltetrahydromethanopterin hydrogenase;
GN   Name=hmd;
OS   Methanococcus voltae.
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanococcaceae; Methanococcus.
OX   NCBI_TaxID=2188;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33273 / DSM 1537 / NBRC 100457 / OCM 70 / PS;
RA   Hartmann G.C., Thauer R.K.;
RL   Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC       methylene-H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-
CC         methylenetetrahydromethanopterin + H(+); Xref=Rhea:RHEA:20017,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18276, ChEBI:CHEBI:57818,
CC         ChEBI:CHEBI:58337; EC=1.12.98.2;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC       methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC       tetrahydromethanopterin (hydrogen route): step 1/1.
CC   -!- SIMILARITY: Belongs to the HMD family. {ECO:0000305}.
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DR   EMBL; X94356; CAA64123.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q50840; -.
DR   SMR; Q50840; -.
DR   UniPathway; UPA00640; UER00696.
DR   GO; GO:0047068; F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.1300; -; 1.
DR   HAMAP; MF_01090; HMD; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR010062; HMD.
DR   InterPro; IPR004889; HMD_C.
DR   InterPro; IPR038182; HMD_C_sf.
DR   InterPro; IPR024190; METHMP_Hmd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF03201; HMD; 1.
DR   PIRSF; PIRSF016158; HMD; 1.
DR   PIRSF; PIRSF500165; HMDI; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01723; hmd_TIGR; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; One-carbon metabolism; Oxidoreductase.
FT   CHAIN           1..343
FT                   /note="5,10-methenyltetrahydromethanopterin hydrogenase"
FT                   /id="PRO_0000218515"
SQ   SEQUENCE   343 AA;  36787 MW;  E25F84E04F12F38C CRC64;
     MKVAILGAGC YRTHAAAGIT NFSRASEVAK QVGIPEIAMT HSTILMGAEL LHLVNEIDEI
     VIADPCFAEG NEMVVLDNFD YAKVMEAHLA GKAEEVMPEI RDAVKAKAKE LPKPPKACIH
     FVNPEKVGLK VTADDKDAVK DADIVITWLP KGGSQPAIIE KFVANIKEGA IVTHACTIPT
     PKFAKIFKDL GRDDLNIISY HPGAVPEMKG QVFISEGFAT EEAVENFFEI ANTARGVAFK
     MPANLISPVC DMGSAVTAPV YAGILAYRDA VTQILGAPAD FAQMMADEAI TQILELMRNE
     GIKGMEDKLD PKALTGTADS MCFGPLADIL PASLKVLEKH GKQ