ID   HMD_METVS               Reviewed;         354 AA.
AC   A6URB0;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=5,10-methenyltetrahydromethanopterin hydrogenase {ECO:0000255|HAMAP-Rule:MF_01090};
DE            EC=1.12.98.2 {ECO:0000255|HAMAP-Rule:MF_01090};
DE   AltName: Full=H(2)-dependent methylene-H(4)MPT dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01090};
DE   AltName: Full=H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01090};
DE   AltName: Full=N(5),N(10)-methenyltetrahydromethanopterin hydrogenase {ECO:0000255|HAMAP-Rule:MF_01090};
GN   Name=hmd {ECO:0000255|HAMAP-Rule:MF_01090}; OrderedLocusNames=Mevan_1132;
OS   Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148
OS   / SB).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanococcaceae; Methanococcus.
OX   NCBI_TaxID=406327;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.,
RA   Sieprawska-Lupa M., Whitman W.B., Richardson P.;
RT   "Complete sequence of Methanococcus vannielii SB.";
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC       methylene-H(4)MPT. {ECO:0000255|HAMAP-Rule:MF_01090}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-
CC         methylenetetrahydromethanopterin + H(+); Xref=Rhea:RHEA:20017,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18276, ChEBI:CHEBI:57818,
CC         ChEBI:CHEBI:58337; EC=1.12.98.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01090};
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC       methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC       tetrahydromethanopterin (hydrogen route): step 1/1. {ECO:0000255|HAMAP-
CC       Rule:MF_01090}.
CC   -!- SIMILARITY: Belongs to the HMD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01090}.
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DR   EMBL; CP000742; ABR55032.1; -; Genomic_DNA.
DR   RefSeq; WP_012065947.1; NC_009634.1.
DR   AlphaFoldDB; A6URB0; -.
DR   SMR; A6URB0; -.
DR   STRING; 406327.Mevan_1132; -.
DR   EnsemblBacteria; ABR55032; ABR55032; Mevan_1132.
DR   GeneID; 5325952; -.
DR   KEGG; mvn:Mevan_1132; -.
DR   eggNOG; arCOG03196; Archaea.
DR   HOGENOM; CLU_772960_0_0_2; -.
DR   OMA; HACTIPT; -.
DR   OrthoDB; 31540at2157; -.
DR   UniPathway; UPA00640; UER00696.
DR   Proteomes; UP000001107; Chromosome.
DR   GO; GO:0047068; F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.1300; -; 1.
DR   HAMAP; MF_01090; HMD; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR010062; HMD.
DR   InterPro; IPR004889; HMD_C.
DR   InterPro; IPR038182; HMD_C_sf.
DR   InterPro; IPR024190; METHMP_Hmd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF03201; HMD; 1.
DR   PIRSF; PIRSF016158; HMD; 1.
DR   PIRSF; PIRSF500165; HMDI; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01723; hmd_TIGR; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; One-carbon metabolism; Oxidoreductase.
FT   CHAIN           1..354
FT                   /note="5,10-methenyltetrahydromethanopterin hydrogenase"
FT                   /id="PRO_1000149887"
SQ   SEQUENCE   354 AA;  37804 MW;  5C9DF1E0278F5452 CRC64;
     MKVAILGAGC YRTHAASGIT NFSRAAQVAK EVGIPEITMT HSTITMGAEL LHLIPEVTEV
     VVSDPCFAEE PGIVVLDQFD YGAVMEAHLA GDAEKVMPEI RAAVKAKAKE TPKPPKGCIH
     FVHPEKVGLK VTSNDVEAVK DADIVITWLP KGGSQPAIIE KFASEIKKGA VVTHACTIPT
     PKFAKIFKDL GRDDLNIISF HPGAVPEMKG QAFLSEGLAD AEKVEEFYCI AKAARGEAFK
     MPANLISPVC DMGSAVTAPV YAAILAYRDA VTQILGAPAD FAQMMADEAI SQMLDLMRKE
     GIKNMEEKLS PKALTGTADS MCFGPLAEIL PASLKVLEKH AKENKCECGC SIKP