HME1_MOUSE
ID HME1_MOUSE Reviewed; 401 AA.
AC P09065; Q3USF7;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=Homeobox protein engrailed-1;
DE Short=Homeobox protein en-1;
DE Short=Mo-En-1;
GN Name=En1; Synonyms=En-1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1363401; DOI=10.1002/dvg.1020130505;
RA Logan C., Hanks M.C., Noble-Topham S., Nallainathan D., Provart N.J.,
RA Joyner A.L.;
RT "Cloning and sequence comparison of the mouse, human, and chicken engrailed
RT genes reveal potential functional domains and regulatory regions.";
RL Dev. Genet. 13:345-358(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 278-401.
RX PubMed=2892757; DOI=10.1101/gad.1.1.29;
RA Joyner A.L., Martin G.R.;
RT "En-1 and En-2, two mouse genes with sequence homology to the Drosophila
RT engrailed gene: expression during embryogenesis.";
RL Genes Dev. 1:29-38(1987).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 298-401.
RX PubMed=2416459; DOI=10.1016/0092-8674(85)90009-1;
RA Joyner A.L., Kornberg T., Coleman K.G., Cox D.R., Martin G.R.;
RT "Expression during embryogenesis of a mouse gene with sequence homology to
RT the Drosophila engrailed gene.";
RL Cell 43:29-37(1985).
RN [5]
RP NUCLEOTIDE SEQUENCE OF 321-380.
RX PubMed=1980115; DOI=10.1016/0014-5793(90)80858-g;
RA Holland P.W.H., Williams N.A.;
RT "Conservation of engrailed-like homeobox sequences during vertebrate
RT evolution.";
RL FEBS Lett. 277:250-252(1990).
RN [6]
RP FUNCTION.
RX PubMed=8684466; DOI=10.1038/382360a0;
RA Loomis C.A., Harris E., Michaud J., Wurst W., Hanks M., Joyner A.L.;
RT "The mouse Engrailed-1 gene and ventral limb patterning.";
RL Nature 382:360-363(1996).
CC -!- FUNCTION: Required for proper formation of the apical ectodermal ridge
CC and correct dorsal-ventral patterning in the limb.
CC {ECO:0000269|PubMed:8684466}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the engrailed homeobox family. {ECO:0000305}.
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DR EMBL; L12703; AAA03660.2; -; mRNA.
DR EMBL; AK140408; BAE24375.1; -; mRNA.
DR EMBL; Y00201; CAA68361.1; -; Genomic_DNA.
DR CCDS; CCDS15235.1; -.
DR PIR; A48423; A48423.
DR RefSeq; NP_034263.2; NM_010133.2.
DR AlphaFoldDB; P09065; -.
DR SMR; P09065; -.
DR BioGRID; 199444; 2.
DR STRING; 10090.ENSMUSP00000078659; -.
DR iPTMnet; P09065; -.
DR PhosphoSitePlus; P09065; -.
DR PaxDb; P09065; -.
DR PRIDE; P09065; -.
DR ProteomicsDB; 273185; -.
DR Antibodypedia; 33365; 256 antibodies from 29 providers.
DR DNASU; 13798; -.
DR Ensembl; ENSMUST00000079721; ENSMUSP00000078659; ENSMUSG00000058665.
DR GeneID; 13798; -.
DR KEGG; mmu:13798; -.
DR UCSC; uc007cjm.2; mouse.
DR CTD; 2019; -.
DR MGI; MGI:95389; En1.
DR VEuPathDB; HostDB:ENSMUSG00000058665; -.
DR eggNOG; KOG0493; Eukaryota.
DR GeneTree; ENSGT00940000160811; -.
DR HOGENOM; CLU_051739_0_1_1; -.
DR InParanoid; P09065; -.
DR OMA; GCKKEPP; -.
DR OrthoDB; 1575614at2759; -.
DR PhylomeDB; P09065; -.
DR TreeFam; TF106461; -.
DR BioGRID-ORCS; 13798; 1 hit in 74 CRISPR screens.
DR PRO; PR:P09065; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P09065; protein.
DR Bgee; ENSMUSG00000058665; Expressed in presumptive midbrain and 105 other tissues.
DR Genevisible; P09065; MM.
DR GO; GO:0016020; C:membrane; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0008344; P:adult locomotory behavior; IMP:ParkinsonsUK-UCL.
DR GO; GO:0021549; P:cerebellum development; IGI:ParkinsonsUK-UCL.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; IGI:MGI.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:MGI.
DR GO; GO:0042756; P:drinking behavior; IMP:ParkinsonsUK-UCL.
DR GO; GO:1990403; P:embryonic brain development; IMP:ParkinsonsUK-UCL.
DR GO; GO:0035115; P:embryonic forelimb morphogenesis; IGI:MGI.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IMP:ParkinsonsUK-UCL.
DR GO; GO:0030902; P:hindbrain development; IGI:MGI.
DR GO; GO:0060173; P:limb development; IMP:MGI.
DR GO; GO:0030901; P:midbrain development; IGI:ParkinsonsUK-UCL.
DR GO; GO:0030917; P:midbrain-hindbrain boundary development; IMP:MGI.
DR GO; GO:0061743; P:motor learning; IMP:ParkinsonsUK-UCL.
DR GO; GO:0035264; P:multicellular organism growth; IMP:MGI.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IGI:ParkinsonsUK-UCL.
DR GO; GO:1901215; P:negative regulation of neuron death; IMP:ParkinsonsUK-UCL.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0048666; P:neuron development; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IGI:MGI.
DR GO; GO:0043473; P:pigmentation; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0009954; P:proximal/distal pattern formation; IMP:MGI.
DR GO; GO:0010468; P:regulation of gene expression; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0042220; P:response to cocaine; IEA:Ensembl.
DR GO; GO:0035176; P:social behavior; IMP:ParkinsonsUK-UCL.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR019549; Homeobox-engrailed_C-terminal.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR000747; Homeobox_engrailed.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR019737; Homoebox-engrailed_CS.
DR Pfam; PF10525; Engrail_1_C_sig; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00026; ENGRAILED.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00033; ENGRAILED; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..401
FT /note="Homeobox protein engrailed-1"
FT /id="PRO_0000196063"
FT DNA_BIND 312..371
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 138..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 293..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..46
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..93
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 197
FT /note="S -> G (in Ref. 1; AAA03660)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 401 AA; 40981 MW; 05456FD3815AA02A CRC64;
MEEQQPEPKS QRDSGLGAVA AAAPSGLSLS LSPGASGSSG SDGDSVPVSP QPAPPSPPAA
PCLPPLAHHP HLPPHPPPPP PPPPPPPQHL AAPAHQPQPA AQLHRTTNFF IDNILRPDFG
CKKEQPLPQL LVASAAAGGG AAAGGGSRVE RDRGQTGAGR DPVHSLGTRA SGAASLLCAP
DANCGPPDGS QPATAVSAGA SKAGNPAAAA AAAAAAAAAA VAAAAAAASK PSDSGGGSGG
NAGSPGAQGA KFPEHNPAIL LMGSANGGPV VKTDSQQPLV WPAWVYCTRY SDRPSSGPRT
RKLKKKKNEK EDKRPRTAFT AEQLQRLKAE FQANRYITEQ RRQTLAQELS LNESQIKIWF
QNKRAKIKKA TGIKNGLALH LMAQGLYNHS TTTVQDKDES E
