HME2A_DANRE
ID HME2A_DANRE Reviewed; 265 AA.
AC P09015;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Homeobox protein engrailed-2a;
DE Short=Homeobox protein en-2a;
DE AltName: Full=Zf-En-2;
GN Name=eng2a; Synonyms=eng-2, eng2, zf-en-2;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1362650; DOI=10.1016/0925-4773(92)90025-f;
RA Fjose A., Njolstad P.R., Nornes S., Molven A., Krauss S.;
RT "Structure and early embryonic expression of the zebrafish engrailed-2
RT gene.";
RL Mech. Dev. 39:51-62(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1363539; DOI=10.1242/dev.116.4.1001;
RA Ekker M., Wegner J., Akimenko M.-A., Westerfield M.;
RT "Coordinate embryonic expression of three zebrafish engrailed genes.";
RL Development 116:1001-1010(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 162-265.
RX PubMed=2896133; DOI=10.1016/0014-5793(88)80849-4;
RA Fjose A., Eiken H.G., Njolstad P.R., Molven A., Hordvik I.;
RT "A zebrafish engrailed-like homeobox sequence expressed during
RT embryogenesis.";
RL FEBS Lett. 231:355-360(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 182-244.
RX PubMed=1980115; DOI=10.1016/0014-5793(90)80858-g;
RA Holland P.W.H., Williams N.A.;
RT "Conservation of engrailed-like homeobox sequences during vertebrate
RT evolution.";
RL FEBS Lett. 277:250-252(1990).
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the engrailed homeobox family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA30471.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X68151; CAA48254.1; -; Genomic_DNA.
DR EMBL; X68446; CAA48491.1; -; mRNA.
DR EMBL; X07622; CAA30471.1; ALT_INIT; Genomic_DNA.
DR EMBL; X59126; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A56557; A56557.
DR PIR; S00562; S00562.
DR RefSeq; NP_571119.2; NM_131044.2.
DR AlphaFoldDB; P09015; -.
DR BMRB; P09015; -.
DR SMR; P09015; -.
DR STRING; 7955.ENSDARP00000028905; -.
DR PaxDb; P09015; -.
DR Ensembl; ENSDART00000031700; ENSDARP00000028905; ENSDARG00000026599.
DR Ensembl; ENSDART00000181260; ENSDARP00000145475; ENSDARG00000115233.
DR GeneID; 30243; -.
DR KEGG; dre:30243; -.
DR CTD; 30243; -.
DR ZFIN; ZDB-GENE-980526-167; en2a.
DR eggNOG; KOG0493; Eukaryota.
DR GeneTree; ENSGT00940000159935; -.
DR HOGENOM; CLU_051739_0_0_1; -.
DR InParanoid; P09015; -.
DR OMA; KHEFQQS; -.
DR OrthoDB; 1575614at2759; -.
DR PhylomeDB; P09015; -.
DR TreeFam; TF106461; -.
DR PRO; PR:P09015; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 7.
DR Bgee; ENSDARG00000026599; Expressed in brain and 6 other tissues.
DR ExpressionAtlas; P09015; baseline.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IGI:ZFIN.
DR GO; GO:0060561; P:apoptotic process involved in morphogenesis; IGI:ZFIN.
DR GO; GO:0060385; P:axonogenesis involved in innervation; IGI:ZFIN.
DR GO; GO:0001708; P:cell fate specification; IGI:ZFIN.
DR GO; GO:0030901; P:midbrain development; IGI:ZFIN.
DR GO; GO:0030917; P:midbrain-hindbrain boundary development; IGI:ZFIN.
DR GO; GO:0021555; P:midbrain-hindbrain boundary morphogenesis; IGI:ZFIN.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR019549; Homeobox-engrailed_C-terminal.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR000747; Homeobox_engrailed.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR019737; Homoebox-engrailed_CS.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF10525; Engrail_1_C_sig; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00026; ENGRAILED.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00033; ENGRAILED; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..265
FT /note="Homeobox protein engrailed-2a"
FT /id="PRO_0000196073"
FT DNA_BIND 176..235
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 65..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 155..180
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 73..102
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 103..117
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 123..142
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 206
FT /note="R -> A (in Ref. 3; CAA30471)"
FT /evidence="ECO:0000305"
FT CONFLICT 265
FT /note="D -> H (in Ref. 2; CAA48491)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 265 AA; 29540 MW; AB4B08EF062D5AE0 CRC64;
MDENEQSARD VEQRGASDES NSAIRPLLQA PGNLQLPHRI TNFFIDNILR PDFGRKKEAN
ITRYEDNHGA RENHNPTGPS TGQVGSTVPA EEASTTHTSS GGKEAEIESE EPLKPRGENV
DQCLGSESDS SQSNSNGQTG QGMLWPAWVY CTRYSDRPSS GPRSRKPKKK AASKEDKRPR
TAFTAEQLQR LKAEFQTNRY LTEQRRQSLA QELGLNESQI KIWFQNKRAK IKKASGVKNG
LAIHLMAQGL YNHSTTSKED KSDSD
