ID   HME2B_DANRE             Reviewed;         261 AA.
AC   P31533;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 2.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Homeobox protein engrailed-2b;
DE            Short=Homeobox protein en-2b;
DE   AltName: Full=Zf-En-1;
GN   Name=eng2b; Synonyms=eng-3, eng3, zf-en-1;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1363539; DOI=10.1242/dev.116.4.1001;
RA   Ekker M., Wegner J., Akimenko M.-A., Westerfield M.;
RT   "Coordinate embryonic expression of three zebrafish engrailed genes.";
RL   Development 116:1001-1010(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 181-240.
RX   PubMed=1980115; DOI=10.1016/0014-5793(90)80858-g;
RA   Holland P.W.H., Williams N.A.;
RT   "Conservation of engrailed-like homeobox sequences during vertebrate
RT   evolution.";
RL   FEBS Lett. 277:250-252(1990).
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the engrailed homeobox family. {ECO:0000305}.
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DR   EMBL; X68447; CAA48492.1; -; mRNA.
DR   EMBL; X59125; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; S30437; S30437.
DR   RefSeq; NP_571115.1; NM_131040.1.
DR   AlphaFoldDB; P31533; -.
DR   SMR; P31533; -.
DR   STRING; 7955.ENSDARP00000056747; -.
DR   PaxDb; P31533; -.
DR   GeneID; 30238; -.
DR   KEGG; dre:30238; -.
DR   CTD; 30238; -.
DR   ZFIN; ZDB-GENE-980526-40; en2b.
DR   eggNOG; KOG0493; Eukaryota.
DR   InParanoid; P31533; -.
DR   OrthoDB; 1575614at2759; -.
DR   PRO; PR:P31533; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0009952; P:anterior/posterior pattern specification; IGI:ZFIN.
DR   GO; GO:0001708; P:cell fate specification; IGI:ZFIN.
DR   GO; GO:0030901; P:midbrain development; IGI:ZFIN.
DR   GO; GO:0030917; P:midbrain-hindbrain boundary development; IGI:ZFIN.
DR   GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR019549; Homeobox-engrailed_C-terminal.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR000747; Homeobox_engrailed.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   InterPro; IPR019737; Homoebox-engrailed_CS.
DR   InterPro; IPR000047; HTH_motif.
DR   Pfam; PF10525; Engrail_1_C_sig; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00026; ENGRAILED.
DR   PRINTS; PR00024; HOMEOBOX.
DR   PRINTS; PR00031; HTHREPRESSR.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00033; ENGRAILED; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..261
FT                   /note="Homeobox protein engrailed-2b"
FT                   /id="PRO_0000196074"
FT   DNA_BIND        172..231
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          53..125
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          152..176
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..19
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        53..69
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        84..103
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   261 AA;  29485 MW;  5362899CB0CA976A CRC64;
     MEENDHSNRD VERQDSGDES NRAILPLLQA PGNVLPHRIT NFYIDNILRP DFGRRKEGSR
     RDEINIVERE NRCPSAPGSG QVAPVSGEGT SSPRAVNASK KTDISTDESL KSRAETGDQC
     LSSDSDCSQR CAAQAKQPML WPAWVYCTRY SDRPSSGPRS RKPKKKTPTK EDKRPRTAFT
     AEQLQRLKNE FQNNRYLTEQ RRQALAQELG LNESQIKIWF QNKRAKIKKA TGNKNTLAVH
     LMAQGLYNHA TVTKDDKSDS D