ID   HME2B_XENLA             Reviewed;         265 AA.
AC   P52730;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Homeobox protein engrailed-2-B;
DE            Short=En-2B;
DE            Short=Homeobox protein en-2-B;
DE   AltName: Full=En2 MABEN;
GN   Name=en2-b; Synonyms=en2b;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Head;
RX   PubMed=1679005; DOI=10.1242/dev.111.3.715;
RA   Hemmati-Brivanlou A., de la Torre J.R., Holt C., Harland R.M.;
RT   "Cephalic expression and molecular characterization of Xenopus En-2.";
RL   Development 111:715-724(1991).
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC   -!- DEVELOPMENTAL STAGE: In addition to the main band of expression at the
CC       midbrain-hindbrain boundary, the protein is expressed in the mandibular
CC       arch, the optic tectum and the region of anterior pituitary.
CC   -!- SIMILARITY: Belongs to the engrailed homeobox family. {ECO:0000305}.
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DR   EMBL; X62974; CAA44724.1; -; mRNA.
DR   PIR; S19005; S19005.
DR   RefSeq; NP_001095261.1; NM_001101791.1.
DR   AlphaFoldDB; P52730; -.
DR   BMRB; P52730; -.
DR   SMR; P52730; -.
DR   PRIDE; P52730; -.
DR   GeneID; 399321; -.
DR   KEGG; xla:399321; -.
DR   CTD; 399321; -.
DR   Xenbase; XB-GENE-6254047; en2.S.
DR   OMA; STSHTHW; -.
DR   OrthoDB; 1575614at2759; -.
DR   Proteomes; UP000186698; Chromosome 6S.
DR   Bgee; 399321; Expressed in neurula embryo and 1 other tissue.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProt.
DR   GO; GO:0007399; P:nervous system development; IEA:UniProt.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR019549; Homeobox-engrailed_C-terminal.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR000747; Homeobox_engrailed.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   InterPro; IPR019737; Homoebox-engrailed_CS.
DR   InterPro; IPR000047; HTH_motif.
DR   Pfam; PF10525; Engrail_1_C_sig; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00026; ENGRAILED.
DR   PRINTS; PR00024; HOMEOBOX.
DR   PRINTS; PR00031; HTHREPRESSR.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00033; ENGRAILED; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..265
FT                   /note="Homeobox protein engrailed-2-B"
FT                   /id="PRO_0000196071"
FT   DNA_BIND        176..235
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          60..138
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          156..182
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        119..138
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   265 AA;  29871 MW;  8FF3B0AAC656A620 CRC64;
     MEENEQNNRE VEPQQESGEE SNRGILHQAP PGNHQPHHRI TNFFIDNILR PEFGRRKERI
     NHQDELFTGR DTGALSGAES GHHRVNVPEG AGGSSKVITV TGEKKSDLAM EETLKSRGLN
     GDHSLSSDSD SSQASSKATQ KPILWPAWVY CTRYSDRPSS GPRSRKPKKK SVSKEDKRPR
     TAFTAEQLQR LKAEFQTNRY LTEQRRQSLA QELGLNESQI KIWFQNKRAK IKKSTGNKNS
     LALHLMAQGL YNHATTSKDG KSDSE