ID   HMEN_DROVI              Reviewed;         584 AA.
AC   P09145;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 124.
DE   RecName: Full=Segmentation polarity homeobox protein engrailed;
GN   Name=en;
OS   Drosophila virilis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila.
OX   NCBI_TaxID=7244;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2881781; DOI=10.1002/j.1460-2075.1986.tb04686.x;
RA   Kassis J.A., Poole S.J., Wright D.K., O'Farrell P.H.;
RT   "Sequence conservation in the protein coding and intron regions of the
RT   engrailed transcription unit.";
RL   EMBO J. 5:3583-3589(1986).
CC   -!- FUNCTION: This protein specifies the body segmentation pattern. It is
CC       required for the development of the central nervous system.
CC       Transcriptional regulator that repress activated promoters.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the engrailed homeobox family. {ECO:0000305}.
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DR   EMBL; X04727; CAA28436.1; -; Genomic_DNA.
DR   PIR; B25682; B25682.
DR   AlphaFoldDB; P09145; -.
DR   BMRB; P09145; -.
DR   SMR; P09145; -.
DR   STRING; 7244.FBpp0236386; -.
DR   eggNOG; KOG0493; Eukaryota.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IEA:EnsemblMetazoa.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IEA:EnsemblMetazoa.
DR   GO; GO:0007411; P:axon guidance; IEA:EnsemblMetazoa.
DR   GO; GO:0035224; P:genital disc anterior/posterior pattern formation; IEA:EnsemblMetazoa.
DR   GO; GO:0008406; P:gonad development; IEA:EnsemblMetazoa.
DR   GO; GO:0007474; P:imaginal disc-derived wing vein specification; IEA:EnsemblMetazoa.
DR   GO; GO:0010629; P:negative regulation of gene expression; IEA:EnsemblMetazoa.
DR   GO; GO:0043524; P:negative regulation of neuron apoptotic process; IEA:EnsemblMetazoa.
DR   GO; GO:0007400; P:neuroblast fate determination; IEA:EnsemblMetazoa.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:EnsemblMetazoa.
DR   GO; GO:0007367; P:segment polarity determination; IEA:UniProtKB-KW.
DR   GO; GO:0035277; P:spiracle morphogenesis, open tracheal system; IEA:EnsemblMetazoa.
DR   GO; GO:0007418; P:ventral midline development; IEA:EnsemblMetazoa.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR019549; Homeobox-engrailed_C-terminal.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR000747; Homeobox_engrailed.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   InterPro; IPR019737; Homoebox-engrailed_CS.
DR   InterPro; IPR000047; HTH_motif.
DR   Pfam; PF10525; Engrail_1_C_sig; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00026; ENGRAILED.
DR   PRINTS; PR00024; HOMEOBOX.
DR   PRINTS; PR00031; HTHREPRESSR.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00033; ENGRAILED; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   3: Inferred from homology;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus;
KW   Segmentation polarity protein.
FT   CHAIN           1..584
FT                   /note="Segmentation polarity homeobox protein engrailed"
FT                   /id="PRO_0000196078"
FT   DNA_BIND        486..545
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          141..198
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          343..380
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          392..451
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          465..492
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        160..174
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        184..198
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        392..414
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        436..450
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        468..492
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   584 AA;  62178 MW;  F556B03D60CD10B7 CRC64;
     MALEDRCSPQ SAPSPPGCLP HSPPQQHQHI LQPNLRIHAI PISITALDMS LQPGTAATAA
     TLYQQQQLQH LHQLQQLQQL HQQQLAASAG VFHHPSTSAA FDAAALHAAA LQQRLSASGS
     PGAHSTGACS TPASTLTIKE EESDSILGDS FHNQTATTAT EEDEEEDDDI DVDDTASVSG
     SARLPPPAHQ QSKQSKPSLA FSISNILSDR FGDAAKQTKQ GDSTGVSVSA AAAAASIFRP
     FEASRAAAAT PSAFTRVDLL EFSRQQQAAA AAATAAMMLE RANLLNCFNP AAYPRIHEEL
     VQSRLRRSAA GNPNAVLPPT AKLTEPSVEK SALGSLCKTV SQIGQAQSTT PVTTPSSRPS
     QLASPPPASN ASTISSSSST AASCSASSSS GCSSAASSLN SSPSSRLAGA NNASSPQPIP
     PPSAVSRDSG MESSDDTRSE TGSTTTDGGK NEMWPAWVYC TRYSDRPSSG PRYRRPKQPK
     DKTNDEKRPR TAFSSEQLAR LKREFNENRY LTERRRQQLS SELGLNEAQI KIWFQNKRAK
     IKKSTGSKNP LALQLMAQGL YNHTTVPLTK EEEELEMRMN GQIP