HMGB4_HUMAN
ID HMGB4_HUMAN Reviewed; 186 AA.
AC Q8WW32; B2R4X7; Q0QWA4;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2012, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=High mobility group protein B4;
GN Name=HMGB4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ALA-92.
RC TISSUE=Testis;
RA Zhou C., Xiao X.W., Zhou J.L., Zhang J.;
RT "Identification of a novel H. sapiens protein with two high-mobility group
RT domains.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ALA-92.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- INTERACTION:
CC Q8WW32; Q8NHQ1: CEP70; NbExp=6; IntAct=EBI-10277297, EBI-739624;
CC Q8WW32; P60329: KRTAP12-4; NbExp=3; IntAct=EBI-10277297, EBI-10176396;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q6P8W9}.
CC Chromosome {ECO:0000250|UniProtKB:Q6P8W9}. Note=Interacts specifically
CC with the sex chromosomes. {ECO:0000250|UniProtKB:Q6P8W9}.
CC -!- SIMILARITY: Belongs to the HMGB family. {ECO:0000305}.
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DR EMBL; DQ207367; ABB22048.1; -; mRNA.
DR EMBL; AK311985; BAG34924.1; -; mRNA.
DR EMBL; AL121980; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC021180; AAH21180.1; -; mRNA.
DR EMBL; CH471059; EAX07453.1; -; Genomic_DNA.
DR CCDS; CCDS30668.1; -.
DR RefSeq; NP_660206.2; NM_145205.5.
DR AlphaFoldDB; Q8WW32; -.
DR SMR; Q8WW32; -.
DR BioGRID; 126064; 4.
DR IntAct; Q8WW32; 3.
DR STRING; 9606.ENSP00000430919; -.
DR iPTMnet; Q8WW32; -.
DR PhosphoSitePlus; Q8WW32; -.
DR BioMuta; HMGB4; -.
DR DMDM; 387912835; -.
DR EPD; Q8WW32; -.
DR MassIVE; Q8WW32; -.
DR PaxDb; Q8WW32; -.
DR PeptideAtlas; Q8WW32; -.
DR PRIDE; Q8WW32; -.
DR ProteomicsDB; 74853; -.
DR Antibodypedia; 17205; 267 antibodies from 29 providers.
DR DNASU; 127540; -.
DR Ensembl; ENST00000519684.5; ENSP00000429214.1; ENSG00000176256.11.
DR Ensembl; ENST00000522796.1; ENSP00000430919.1; ENSG00000176256.11.
DR Ensembl; ENST00000681531.1; ENSP00000505691.1; ENSG00000176256.11.
DR GeneID; 127540; -.
DR KEGG; hsa:127540; -.
DR MANE-Select; ENST00000681531.1; ENSP00000505691.1; NM_001379301.1; NP_001366230.1.
DR UCSC; uc001bxp.3; human.
DR CTD; 127540; -.
DR DisGeNET; 127540; -.
DR GeneCards; HMGB4; -.
DR HGNC; HGNC:24954; HMGB4.
DR HPA; ENSG00000176256; Tissue enriched (testis).
DR neXtProt; NX_Q8WW32; -.
DR OpenTargets; ENSG00000176256; -.
DR VEuPathDB; HostDB:ENSG00000176256; -.
DR eggNOG; KOG0381; Eukaryota.
DR GeneTree; ENSGT00940000162735; -.
DR HOGENOM; CLU_082854_0_4_1; -.
DR InParanoid; Q8WW32; -.
DR OMA; KMWSAKT; -.
DR OrthoDB; 1641977at2759; -.
DR PhylomeDB; Q8WW32; -.
DR TreeFam; TF105371; -.
DR PathwayCommons; Q8WW32; -.
DR SignaLink; Q8WW32; -.
DR BioGRID-ORCS; 127540; 8 hits in 1068 CRISPR screens.
DR GeneWiki; HMGB4; -.
DR GenomeRNAi; 127540; -.
DR Pharos; Q8WW32; Tbio.
DR PRO; PR:Q8WW32; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q8WW32; protein.
DR Bgee; ENSG00000176256; Expressed in right testis and 106 other tissues.
DR Genevisible; Q8WW32; HS.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:0008301; F:DNA binding, bending; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.30.10; -; 2.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR Pfam; PF00505; HMG_box; 1.
DR Pfam; PF09011; HMG_box_2; 1.
DR SMART; SM00398; HMG; 2.
DR SUPFAM; SSF47095; SSF47095; 2.
DR PROSITE; PS50118; HMG_BOX_2; 2.
PE 1: Evidence at protein level;
KW Chromosome; DNA-binding; Nucleus; Reference proteome; Repeat.
FT CHAIN 1..186
FT /note="High mobility group protein B4"
FT /id="PRO_0000269180"
FT DNA_BIND 9..79
FT /note="HMG box 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT DNA_BIND 93..161
FT /note="HMG box 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 77..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 59
FT /note="K -> R (in dbSNP:rs11542686)"
FT /id="VAR_055951"
FT VARIANT 92
FT /note="E -> A (in dbSNP:rs10379)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.1"
FT /id="VAR_067467"
FT VARIANT 170
FT /note="Y -> H (in dbSNP:rs57068937)"
FT /id="VAR_067468"
FT CONFLICT 33
FT /note="N -> S (in Ref. 5; AAH21180)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 186 AA; 22490 MW; E7F941ABBC8D3025 CRC64;
MGKEIQLKPK ANVSSYVHFL LNYRNKFKEQ QPNTYVGFKE FSRKCSEKWR SISKHEKAKY
EALAKLDKAR YQEEMMNYVG KRKKRRKRDP QEPRRPPSSF LLFCQDHYAQ LKRENPNWSV
VQVAKATGKM WSTATDLEKH PYEQRVALLR AKYFEELELY RKQCNARKKY RMSARNRCRG
KRVRQS
