HMGB7_ARATH
ID HMGB7_ARATH Reviewed; 241 AA.
AC Q8LDF9; Q9FHL6;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=High mobility group B protein 7;
DE AltName: Full=Nucleosome/chromatin assembly factor group D 07;
DE Short=Nucleosome/chromatin assembly factor group D 7;
DE AltName: Full=Protein NUCLEAR FUSION DEFECTIVE 7 {ECO:0000303|PubMed:16698901};
GN Name=HMGB7; Synonyms=HMGB6, NFD7 {ECO:0000303|PubMed:16698901};
GN OrderedLocusNames=At5g23420; ORFNames=K19M13.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND PHOSPHORYLATION.
RX PubMed=14756567; DOI=10.1021/bi035931c;
RA Grasser K.D., Grill S., Duroux M., Launholt D., Thomsen M.S., Nielsen B.V.,
RA Nielsen H.K., Merkle T.;
RT "HMGB6 from Arabidopsis thaliana specifies a novel type of plant
RT chromosomal HMGB protein.";
RL Biochemistry 43:1309-1314(2004).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=16698901; DOI=10.1104/pp.106.079319;
RA Portereiko M.F., Sandaklie-Nikolova L., Lloyd A., Dever C.A., Otsuga D.,
RA Drews G.N.;
RT "NUCLEAR FUSION DEFECTIVE1 encodes the Arabidopsis RPL21M protein and is
RT required for karyogamy during female gametophyte development and
RT fertilization.";
RL Plant Physiol. 141:957-965(2006).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=17316617; DOI=10.1016/j.febslet.2007.02.015;
RA Launholt D., Groenlund J.T., Nielsen H.K., Grasser K.D.;
RT "Overlapping expression patterns among the genes encoding Arabidopsis
RT chromosomal high mobility group (HMG) proteins.";
RL FEBS Lett. 581:1114-1118(2007).
RN [8]
RP REVIEW.
RX PubMed=20123078; DOI=10.1016/j.bbagrm.2009.11.004;
RA Pedersen D.S., Grasser K.D.;
RT "The role of chromosomal HMGB proteins in plants.";
RL Biochim. Biophys. Acta 1799:171-174(2010).
CC -!- FUNCTION: Binds preferentially double-stranded supercoiled DNA
CC (PubMed:14756567). Required for karyogamy during female gametophyte
CC development, when the two polar nuclei fuse to form the diploid central
CC cell nucleus (PubMed:16698901). {ECO:0000269|PubMed:14756567,
CC ECO:0000269|PubMed:16698901}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267,
CC ECO:0000269|PubMed:14756567}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8LDF9-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed at low levels in lateral roots, root
CC tips, cotyledons, leaves and flowers (including pedicels, but excluding
CC styles). {ECO:0000269|PubMed:17316617}.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:14756567}.
CC -!- DISRUPTION PHENOTYPE: Failure of fusion of the polar nuclei during
CC megagametogenesis. {ECO:0000269|PubMed:16698901}.
CC -!- SIMILARITY: Belongs to the HMGB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09558.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB018110; BAB09558.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED93164.1; -; Genomic_DNA.
DR EMBL; AK118688; BAC43282.1; -; mRNA.
DR EMBL; AY086023; AAM63233.1; -; mRNA.
DR RefSeq; NP_568431.1; NM_122249.4. [Q8LDF9-1]
DR AlphaFoldDB; Q8LDF9; -.
DR SMR; Q8LDF9; -.
DR STRING; 3702.AT5G23420.1; -.
DR iPTMnet; Q8LDF9; -.
DR PaxDb; Q8LDF9; -.
DR PRIDE; Q8LDF9; -.
DR ProteomicsDB; 228806; -. [Q8LDF9-1]
DR EnsemblPlants; AT5G23420.1; AT5G23420.1; AT5G23420. [Q8LDF9-1]
DR GeneID; 832408; -.
DR Gramene; AT5G23420.1; AT5G23420.1; AT5G23420. [Q8LDF9-1]
DR KEGG; ath:AT5G23420; -.
DR Araport; AT5G23420; -.
DR TAIR; locus:2154433; AT5G23420.
DR eggNOG; KOG0381; Eukaryota.
DR HOGENOM; CLU_097693_0_0_1; -.
DR InParanoid; Q8LDF9; -.
DR OMA; WAQYESK; -.
DR OrthoDB; 1641977at2759; -.
DR PhylomeDB; Q8LDF9; -.
DR PRO; PR:Q8LDF9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8LDF9; baseline and differential.
DR Genevisible; Q8LDF9; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000741; P:karyogamy; IMP:UniProtKB.
DR GO; GO:0010197; P:polar nucleus fusion; IMP:UniProtKB.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Karyogamy;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..241
FT /note="High mobility group B protein 7"
FT /id="PRO_0000399933"
FT DNA_BIND 115..183
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 75..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 174..241
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..112
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..217
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 227..241
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 241 AA; 26982 MW; C7CE66A84F203855 CRC64;
MAGPSTTSNA PKQRKRVEAE TSSNTSTTLR RAKDGSAFAL CEGCNKSVAV ALISMHNCSL
DAKIRVNLEA QVVETQAEAK KKPAEKKKTT SDGPKPKRLK KTNDEKKSSS TSNKPKRPLT
AFFIFMSDFR KTFKSEHNGS LAKDAAKIGG EKWKSLTEEE KKVYLDKAAE LKAEYNKSLE
SNDADEEEED EEKQSDDVDD AEEKQVDDDD EVEEKEVENT DDDKKEAEGK EEEEEEILDD
Y
