HMGB9_ARATH
ID HMGB9_ARATH Reviewed; 338 AA.
AC Q9SGS2;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=High mobility group B protein 9;
DE AltName: Full=Nucleosome/chromatin assembly factor group D 09;
DE Short=Nucleosome/chromatin assembly factor group D 9;
DE AltName: Full=Protein NUCLEAR FUSION DEFECTIVE 9 {ECO:0000303|PubMed:16698901};
GN Name=HMGB9; Synonyms=ARID-HMG1, NFD9 {ECO:0000303|PubMed:16698901};
GN OrderedLocusNames=At1g76110; ORFNames=T23E18.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=16698901; DOI=10.1104/pp.106.079319;
RA Portereiko M.F., Sandaklie-Nikolova L., Lloyd A., Dever C.A., Otsuga D.,
RA Drews G.N.;
RT "NUCLEAR FUSION DEFECTIVE1 encodes the Arabidopsis RPL21M protein and is
RT required for karyogamy during female gametophyte development and
RT fertilization.";
RL Plant Physiol. 141:957-965(2006).
RN [5]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=19053246; DOI=10.1021/bi801772k;
RA Hansen F.T., Madsen C.K., Nordland A.M., Grasser M., Merkle T.,
RA Grasser K.D.;
RT "A novel family of plant DNA-binding proteins containing both HMG-box and
RT AT-rich interaction domains.";
RL Biochemistry 47:13207-13214(2008).
CC -!- FUNCTION: Binds preferentially DNA with A/T-rich content
CC (PubMed:19053246). Required for karyogamy during female gametophyte
CC development, when the two polar nuclei fuse to form the diploid central
CC cell nucleus (PubMed:16698901). {ECO:0000269|PubMed:16698901,
CC ECO:0000269|PubMed:19053246}.
CC -!- INTERACTION:
CC Q9SGS2; Q9LG02: HMGB11; NbExp=3; IntAct=EBI-4425221, EBI-15201560;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267,
CC ECO:0000255|PROSITE-ProRule:PRU00355, ECO:0000269|PubMed:19053246}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in leaves, flowers and
CC seedlings. {ECO:0000269|PubMed:19053246}.
CC -!- DISRUPTION PHENOTYPE: Failure of fusion of the polar nuclei during
CC megagametogenesis. {ECO:0000269|PubMed:16698901}.
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DR EMBL; AC009978; AAF17649.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35797.1; -; Genomic_DNA.
DR EMBL; AY099630; AAM20481.1; -; mRNA.
DR EMBL; BT008823; AAP68262.1; -; mRNA.
DR PIR; B96789; B96789.
DR RefSeq; NP_177738.1; NM_106260.3.
DR AlphaFoldDB; Q9SGS2; -.
DR SMR; Q9SGS2; -.
DR BioGRID; 29162; 4.
DR IntAct; Q9SGS2; 4.
DR STRING; 3702.AT1G76110.1; -.
DR PaxDb; Q9SGS2; -.
DR PRIDE; Q9SGS2; -.
DR ProteomicsDB; 230257; -.
DR EnsemblPlants; AT1G76110.1; AT1G76110.1; AT1G76110.
DR GeneID; 843943; -.
DR Gramene; AT1G76110.1; AT1G76110.1; AT1G76110.
DR KEGG; ath:AT1G76110; -.
DR Araport; AT1G76110; -.
DR TAIR; locus:2199824; AT1G76110.
DR eggNOG; KOG0381; Eukaryota.
DR eggNOG; KOG2744; Eukaryota.
DR HOGENOM; CLU_035371_0_0_1; -.
DR InParanoid; Q9SGS2; -.
DR OMA; IMGTKFM; -.
DR OrthoDB; 891562at2759; -.
DR PhylomeDB; Q9SGS2; -.
DR PRO; PR:Q9SGS2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SGS2; baseline and differential.
DR Genevisible; Q9SGS2; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000741; P:karyogamy; IMP:UniProtKB.
DR GO; GO:0010197; P:polar nucleus fusion; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR CDD; cd16872; ARID_HMGB9-like; 1.
DR Gene3D; 1.10.150.60; -; 1.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR001606; ARID_dom.
DR InterPro; IPR036431; ARID_dom_sf.
DR InterPro; IPR045303; ARID_HMGB9-like.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR Pfam; PF01388; ARID; 1.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00501; BRIGHT; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF46774; SSF46774; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS51011; ARID; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Karyogamy; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..338
FT /note="High mobility group B protein 9"
FT /id="PRO_0000399934"
FT DOMAIN 38..129
FT /note="ARID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00355"
FT DNA_BIND 255..322
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 233..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 338 AA; 38774 MW; EA54C0EC7AD39070 CRC64;
MSSDNESTPS QATVEMMATS PAKIKEYPEP LALHEVVVKD SSVFWDTLRR FHSIMSTKFM
IPVIGGKELD LHVLYVEVTR RGGYEKVVVE KKWREVGGVF RFSATTTSAS FVLRKHYLNL
LFHYEQVHLF TARGPLLHPI ATFHANPSTS KEMALVEYTP PSIRYHNTHP PSQGSSSFTA
IGTIEGKFDC GYLVKVKLGS EILNGVLYHS AQPGPSSSPT AVLNNAVVPY VETGRRRRRL
GKRRRSRRRE DPNYPKPNRS GYNFFFAEKH CKLKSLYPNK EREFTKLIGE SWSNLSTEER
MVYQDIGLKD KERYQRELNE YRETLRLRDG DMTNGKAF
