ID   HMGB_TETTH              Reviewed;         143 AA.
AC   P40626; P12072;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=High mobility group protein B;
DE   AltName: Full=Non-histone chromosomal protein LG-2;
OS   Tetrahymena thermophila.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC   Tetrahymena.
OX   NCBI_TaxID=5911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1986218; DOI=10.1128/mcb.11.1.166-174.1991;
RA   Schulman I.G., Wang T., Wu M., Bowen J., Cook R.G., Gorovsky M.A.,
RA   Allis C.D.;
RT   "Macronuclei and micronuclei in Tetrahymena thermophila contain high-
RT   mobility-group-like chromosomal proteins containing a highly conserved
RT   eleven-amino-acid putative DNA-binding sequence.";
RL   Mol. Cell. Biol. 11:166-174(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 70-96 AND 118-141.
RX   PubMed=3584238; DOI=10.1083/jcb.104.6.1485;
RA   Schulman I.G., Cook R.G., Richman R., Allis C.D.;
RT   "Tetrahymena contain two distinct and unusual high mobility group (HMG)-
RT   like proteins.";
RL   J. Cell Biol. 104:1485-1494(1987).
CC   -!- SUBCELLULAR LOCATION: Nucleus. Chromosome. Note=Macronuclei.
CC   -!- CAUTION: It is not sure if the block at the amino end is natural or
CC       artifactual. {ECO:0000305}.
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DR   EMBL; M63425; AAA30121.1; -; Genomic_DNA.
DR   PIR; B27191; B27191.
DR   PIR; B39668; B39668.
DR   AlphaFoldDB; P40626; -.
DR   SMR; P40626; -.
DR   GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   Pfam; PF00505; HMG_box; 1.
DR   SMART; SM00398; HMG; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
DR   PROSITE; PS50118; HMG_BOX_2; 1.
PE   1: Evidence at protein level;
KW   Chromosome; Direct protein sequencing; DNA-binding; Nucleus.
FT   CHAIN           1..143
FT                   /note="High mobility group protein B"
FT                   /id="PRO_0000048561"
FT   DNA_BIND        18..86
FT                   /note="HMG box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          100..143
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        100..136
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         70
FT                   /note="Blocked amino end (Ala)"
FT   CONFLICT        123..124
FT                   /note="KK -> AP (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        128
FT                   /note="A -> P (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        132..133
FT                   /note="KK -> DD (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        136..137
FT                   /note="KK -> AP (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   143 AA;  16473 MW;  23A91C16F8DE4757 CRC64;
     MSKAASQYAT LEDLPSKPKR PQTGFFIYKS EVFAKRRTEC PTLKVPEIVS KISEEYKALP
     EKEKQKYEEA YRKEKATYDK QNDQWKEKYG DIEKSLKDQA KKALKEKTKK SKAAEKELEK
     SKKKAPAAAP AKKDDKKAPA KKK