ID   AN_HCMVM                Reviewed;         584 AA.
AC   F5HF49;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   23-FEB-2022, entry version 35.
DE   RecName: Full=Alkaline nuclease {ECO:0000255|HAMAP-Rule:MF_04009};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_04009};
GN   Name=UL98;
OS   Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus.
OX   NCBI_TaxID=295027;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15105547; DOI=10.1099/vir.0.79888-0;
RA   Dolan A., Cunningham C., Hector R.D., Hassan-Walker A.F., Lee L.,
RA   Addison C., Dargan D.J., McGeoch D.J., Gatherer D., Emery V.C.,
RA   Griffiths P.D., Sinzger C., McSharry B.P., Wilkinson G.W.G., Davison A.J.;
RT   "Genetic content of wild-type human cytomegalovirus.";
RL   J. Gen. Virol. 85:1301-1312(2004).
CC   -!- FUNCTION: Plays a role in processing non linear or branched viral DNA
CC       intermediates in order to promote the production of mature packaged
CC       unit-length linear progeny viral DNA molecules. Exhibits endonuclease
CC       and exonuclease activities and accepts both double-stranded and single-
CC       stranded DNA as substrate. Exonuclease digestion of DNA is in the 5'->
CC       3' direction and the products are 5'-monophosphate nucleosides.
CC       Additionally, forms a recombinase with the major DNA-binding protein,
CC       which displays strand exchange activity. {ECO:0000255|HAMAP-
CC       Rule:MF_04009}.
CC   -!- SUBUNIT: Interacts with major DNA-binding protein; this interaction
CC       increases the nuclease processivity of the alkaline exonuclease.
CC       {ECO:0000255|HAMAP-Rule:MF_04009}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04009}.
CC       Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04009}.
CC   -!- SIMILARITY: Belongs to the herpesviridae alkaline nuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_04009}.
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DR   EMBL; AY446894; AAR31649.1; -; Genomic_DNA.
DR   RefSeq; YP_081545.1; NC_006273.2.
DR   SMR; F5HF49; -.
DR   PRIDE; F5HF49; -.
DR   GeneID; 3077527; -.
DR   KEGG; vg:3077527; -.
DR   Reactome; R-HSA-9609690; HCMV Early Events.
DR   Proteomes; UP000000938; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004520; F:endodeoxyribonuclease activity; IDA:CACAO.
DR   GO; GO:0004529; F:exodeoxyribonuclease activity; IDA:CACAO.
DR   GO; GO:0016032; P:viral process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04009; HSV_AN; 1.
DR   InterPro; IPR001616; Herpes_alk_exo.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR034720; Viral_alk_exo.
DR   Pfam; PF01771; Viral_alk_exo; 1.
DR   PRINTS; PR00924; ALKEXNUCLASE.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Exonuclease; Host cytoplasm; Host nucleus;
KW   Host-virus interaction; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..584
FT                   /note="Alkaline nuclease"
FT                   /id="PRO_0000417829"
FT   REGION          409..429
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            216
FT                   /note="Required for function"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04009"
FT   SITE            254
FT                   /note="Required for function"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04009"
FT   SITE            278
FT                   /note="Required for function"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04009"
FT   SITE            280
FT                   /note="Required for function"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04009"
SQ   SEQUENCE   584 AA;  65313 MW;  3B5A571DA4253118 CRC64;
     MWGVSSLDYD DDEELTRLLA VWDDEPLSLF LMNTFLLHQE GFRNLPFTVL RLSYAYRIFA
     KMLRAHGTPV AEDFMTRVAA LARDEGLRDI LGQRHAAEAS RAEIAEALER VAERCDDRHG
     GSDDYVWLSR LLDLAPNYRQ VELFQLLEKE SRGQSRNSVW HLLRMDTVSA TKFYEAFVSG
     CLPGAAAADG SGGGGSHYTG SRAGVSPGIQ FGIKHEGLVK TLVECYVMHG REPVRDGLGL
     LIDPTSGLLG ASMDLCFGVL KQGSGRTLLV EPCARVYEIK CRYKYLRKKE DPFVQNVLRR
     HDAAAVASLL QSHPVPGVEF RGERETPSAR EFLLSHDAAL FRATLKRARP LKPPEPLREY
     LADLLYLNKA ECSEVIVFDA KHLNDDNSDG DATITINASL GLAAGDAAGG GADHHLRGSP
     GDSPPPIPFE DENTPELLGR LNVYEVARFS LPAFVNPRHQ YYFQMLIQQY VLSQYYIKKH
     PDPERIDFRD LPTVYLVSAI FREREESELG CELLAGGRVF HCDHIPLLLI VTPVVFDPQF
     TRHAVSTVLD RWSRDLSRKT NLPIWVPNSA NEYVVSSVPR PVSP