ID   AN_NPVAC                Reviewed;         419 AA.
AC   P24731;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   23-FEB-2022, entry version 73.
DE   RecName: Full=Alkaline nuclease;
DE            Short=AN;
DE            EC=3.1.-.-;
GN   Name=AN; ORFNames=ORF133;
OS   Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=46015;
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3041026; DOI=10.1128/jvi.61.10.3048-3057.1987;
RA   Oellig C., Happ B., Mueller T., Doerfler W.;
RT   "Overlapping sets of viral RNAs reflect the array of polypeptides in the
RT   EcoRI J and N fragments (map positions 81.2 to 85.0) of the Autographa
RT   californica nuclear polyhedrosis virus genome.";
RL   J. Virol. 61:3048-3057(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C6;
RX   PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA   Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT   "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT   virus.";
RL   Virology 202:586-605(1994).
RN   [3]
RP   FUNCTION, AND MUTAGENESIS OF GLY-141 AND SER-146.
RC   STRAIN=E2;
RX   PubMed=10864651; DOI=10.1128/jvi.74.14.6401-6407.2000;
RA   Li L., Rohrmann G.F.;
RT   "Characterization of a baculovirus alkaline nuclease.";
RL   J. Virol. 74:6401-6407(2000).
RN   [4]
RP   INTERACTION WITH LEF-3.
RC   STRAIN=E2;
RX   PubMed=12551981; DOI=10.1128/jvi.77.4.2436-2444.2003;
RA   Mikhailov V.S., Okano K., Rohrmann G.F.;
RT   "Baculovirus alkaline nuclease possesses a 5'-->3' exonuclease activity and
RT   associates with the DNA-binding protein LEF-3.";
RL   J. Virol. 77:2436-2444(2003).
RN   [5]
RP   FUNCTION, AND ENDONUCLEASE ACTIVITY.
RC   STRAIN=E2;
RX   PubMed=14736888; DOI=10.1074/jbc.m311658200;
RA   Mikhailov V.S., Okano K., Rohrmann G.F.;
RT   "Specificity of the endonuclease activity of the baculovirus alkaline
RT   nuclease for single-stranded DNA.";
RL   J. Biol. Chem. 279:14734-14745(2004).
RN   [6]
RP   FUNCTION.
RX   PubMed=15367632; DOI=10.1128/jvi.78.19.10650-10656.2004;
RA   Okano K., Vanarsdall A.L., Rohrmann G.F.;
RT   "Characterization of a baculovirus lacking the alkaline nuclease gene.";
RL   J. Virol. 78:10650-10656(2004).
RN   [7]
RP   FUNCTION.
RX   PubMed=17046043; DOI=10.1016/j.virol.2006.09.008;
RA   Okano K., Vanarsdall A.L., Rohrmann G.F.;
RT   "A baculovirus alkaline nuclease knockout construct produces fragmented DNA
RT   and aberrant capsids.";
RL   Virology 359:46-54(2007).
CC   -!- FUNCTION: May play a role in maturation and encapsidation of viral
CC       replicated genome, by promoting DNA homologous recombination. Exhibits
CC       endonuclease and 5'->3' exonuclease activities. The endonuclease
CC       activity displays a specificity for ssDNA in vitro.
CC       {ECO:0000269|PubMed:10864651, ECO:0000269|PubMed:14736888,
CC       ECO:0000269|PubMed:15367632, ECO:0000269|PubMed:17046043}.
CC   -!- SUBUNIT: Interacts with LEF-3. {ECO:0000269|PubMed:12551981}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the baculo-herpesviridae alkaline nuclease
CC       family. {ECO:0000305}.
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DR   EMBL; M17548; AAA66807.1; -; Genomic_DNA.
DR   EMBL; L22858; AAA66763.1; -; Genomic_DNA.
DR   PIR; F72866; F72866.
DR   RefSeq; NP_054163.1; NC_001623.1.
DR   SMR; P24731; -.
DR   PRIDE; P24731; -.
DR   GeneID; 1403966; -.
DR   KEGG; vg:1403966; -.
DR   Proteomes; UP000008292; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.320.10; -; 1.
DR   InterPro; IPR011604; PDDEXK-like_dom_sf.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR034720; Viral_alk_exo.
DR   Pfam; PF01771; Viral_alk_exo; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   1: Evidence at protein level;
KW   Endonuclease; Exonuclease; Host nucleus; Hydrolase; Late protein; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..419
FT                   /note="Alkaline nuclease"
FT                   /id="PRO_0000132811"
FT   MUTAGEN         141
FT                   /note="G->A: 65% loss of alkaline exonuclease activity."
FT                   /evidence="ECO:0000269|PubMed:10864651"
FT   MUTAGEN         146
FT                   /note="S->A: 90% loss of alkaline exonuclease activity."
FT                   /evidence="ECO:0000269|PubMed:10864651"
SQ   SEQUENCE   419 AA;  48291 MW;  BB8E66ED8C058BDE CRC64;
     MFASLTSEQK LLLKKYKFNN YVKTIELSQA QLAHWRSNKD IQPKPLDRAE ILRVEKATRG
     QSKNELWTLL RLDRNTASAS SNSSGNMLQR PALLFGNAQE SHVKETNGIM LDHMREIIES
     KIMSAVVETV LDCGMFFSPL GLHAASPDAY FSLADGTWIP VEIKCPYNYR DTTVEQMRVE
     LGNGNRKYRV KHTALLVNKK GTPQFEMVKT DAHYKQMQRQ MYVMNAPMGF YVVKFKQNLV
     VVSVPRDETF CNKELSTENN AYVAFAVENS NCARYQCADK RRLSFKTHSC NHNYSGQEID
     AMVDRGIYLD YGHLKCAYCD FSSDSRETCD SVLKREHTNC KSFNLKHKNF DNPTYFDYVK
     RLQSLLKSHH FRNDAKTLAY FGYYLTHTGT LKTFCCGSQN SSPTKHDHLN DCVYYLEIK