ID   AN_NPVOP                Reviewed;         424 AA.
AC   P24081;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Alkaline nuclease;
DE            Short=AN;
DE            EC=3.1.-.-;
GN   Name=ALK-EXO; ORFNames=ORF131;
OS   Orgyia pseudotsugata multicapsid polyhedrosis virus (OpMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=262177;
OH   NCBI_TaxID=33414; Orgyia pseudotsugata (Douglas-fir tussock moth).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2661722; DOI=10.1099/0022-1317-70-7-1815;
RA   Gombart A.F., Blissard G.W., Rohrmann G.F.;
RT   "Characterization of the genetic organization of the HindIII M region of
RT   the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata reveals
RT   major differences among baculoviruses.";
RL   J. Gen. Virol. 70:1815-1828(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9126251; DOI=10.1006/viro.1997.8448;
RA   Ahrens C.H., Russell R.R., Funk C.J., Evans J., Harwood S., Rohrmann G.F.;
RT   "The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear
RT   polyhedrosis virus genome.";
RL   Virology 229:381-399(1997).
CC   -!- FUNCTION: May play a role in maturation and encapsidation of viral
CC       replicated genome, by promoting DNA homologous recombination. Exhibits
CC       endonuclease and 5'->3' exonuclease activities. The endonuclease
CC       activity displays a specificity for ssDNA in vitro (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with LEF-3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the baculo-herpesviridae alkaline nuclease
CC       family. {ECO:0000305}.
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DR   EMBL; D13796; BAA02953.1; -; Genomic_DNA.
DR   EMBL; D13929; BAA03031.1; -; Genomic_DNA.
DR   EMBL; U75930; AAC59130.1; -; Genomic_DNA.
DR   PIR; E30857; E30857.
DR   RefSeq; NP_046287.1; NC_001875.2.
DR   PRIDE; P24081; -.
DR   GeneID; 911977; -.
DR   KEGG; vg:911977; -.
DR   Proteomes; UP000009248; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.320.10; -; 1.
DR   InterPro; IPR011604; PDDEXK-like_dom_sf.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR034720; Viral_alk_exo.
DR   Pfam; PF01771; Viral_alk_exo; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Exonuclease; Host nucleus; Hydrolase; Late protein; Nuclease.
FT   CHAIN           1..424
FT                   /note="Alkaline nuclease"
FT                   /id="PRO_0000132812"
SQ   SEQUENCE   424 AA;  47351 MW;  57C8EBBCF1C046AF CRC64;
     MHAPLTAEQR DVYDKYKFAT YARSVALTRA QLDQWRDNKV IVPEPVSRAE TLRVEAATRG
     QSKNALWNLL RLDRSTASRS SGGVALRSSA LAFGNAQENR LKLANGELFE RLGRLAAERA
     GCPVAETVLD CGMFISAFGL HSASPDAYFA MADGSCVPVE IKCPFNYRDT TVDQMRLELG
     KANRKYRVKH TALLVNKAGP AQFEVVKTHD HYRQMQRQMY VMRNAPVCFY VVRFKHNLVA
     LAVPRDDDFC RKEAAAEGAA FVAFATENAG RVQFKRGDRR RASFAQNAAD HGYNAAQVDA
     LVRRGLYLSY GQLRCGHCDA FALDGPRAFE LAMARPHEQC DGLALQEHEF DNVAFLDFTK
     RYTSLVDKRC DDARALRVDG FYVDDAGAVK TFCCGVHGSN ASRRHLPTCS YYLAMGVNKI
     QNNM