HMSH_DROME
ID HMSH_DROME Reviewed; 515 AA.
AC Q03372; Q24481; Q8T0H1; Q9VAK4;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Muscle segmentation homeobox;
DE AltName: Full=Protein drop;
DE AltName: Full=Protein msh;
GN Name=Dr; Synonyms=msh; ORFNames=CG1897;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S; TISSUE=Embryo;
RX PubMed=7556942; DOI=10.1006/dbio.1995.1310;
RA Lord P.C.W., Lin M.H., Hales K.H., Storti R.V.;
RT "Normal expression and the effects of ectopic expression of the Drosophila
RT muscle segment homeobox (msh) gene suggest a role in differentiation and
RT patterning of embryonic muscles.";
RL Dev. Biol. 171:627-640(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=8887329; DOI=10.1016/s0925-4773(96)00583-7;
RA D'Alessio M., Frasch M.;
RT "msh may play a conserved role in dorsoventral patterning of the
RT neuroectoderm and mesoderm.";
RL Mech. Dev. 58:217-231(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S;
RX PubMed=9486795; DOI=10.1242/dev.125.2.215;
RA Nose A., Isshiki T., Takeichi M.;
RT "Regional specification of muscle progenitors in Drosophila: the role of
RT the msh homeobox gene.";
RL Development 125:215-223(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 420-480.
RC STRAIN=Oregon-R;
RX PubMed=1673109; DOI=10.1016/0378-1119(91)90182-b;
RA Holland P.W.H.;
RT "Cloning and evolutionary analysis of msh-like homeobox genes from mouse,
RT zebrafish and ascidian.";
RL Gene 98:253-257(1991).
RN [8]
RP FUNCTION, AND MUTAGENESIS OF 68-HIS--GLY-515.
RX PubMed=28716930; DOI=10.1073/pnas.1704194114;
RA Li Y., Zhao D., Horie T., Chen G., Bao H., Chen S., Liu W., Horie R.,
RA Liang T., Dong B., Feng Q., Tao Q., Liu X.;
RT "Conserved gene regulatory module specifies lateral neural borders across
RT bilaterians.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:6352-6360(2017).
CC -!- FUNCTION: Plays a key role in the specification of proneural and
CC promuscular cluster formation (PubMed:8887329, PubMed:9486795).
CC Required for the specification of dorsal and lateral muscle progenitor
CC cells (PubMed:8887329, PubMed:9486795). Regulates development of
CC peripheral nervous system derived from lateral neuroblasts
CC (PubMed:28716930). {ECO:0000269|PubMed:28716930,
CC ECO:0000269|PubMed:8887329, ECO:0000269|PubMed:9486795}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Dorsal lateral ectoderm, developing central and
CC peripheral nervous systems and the somatic mesoderm. Expressed in
CC dorsal and lateral muscle preclusters and mesodermal fat body
CC precursors. {ECO:0000269|PubMed:7556942, ECO:0000269|PubMed:8887329,
CC ECO:0000269|PubMed:9486795}.
CC -!- DEVELOPMENTAL STAGE: Embryo, between 4 hours and larval first instar
CC whereupon expression is greatly reduced. Continued expression only in
CC the CNS up until hatching. {ECO:0000269|PubMed:7556942,
CC ECO:0000269|PubMed:8887329}.
CC -!- SIMILARITY: Belongs to the Msh homeobox family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA59680.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X85331; CAA59680.1; ALT_FRAME; mRNA.
DR EMBL; U33319; AAC47329.1; -; mRNA.
DR EMBL; AF009038; AAB62975.1; -; mRNA.
DR EMBL; AE014297; AAF56902.2; -; Genomic_DNA.
DR EMBL; AY069324; AAL39469.1; -; mRNA.
DR EMBL; M38582; AAA28611.1; -; Genomic_DNA.
DR PIR; PS0404; PS0404.
DR PIR; S55392; S55392.
DR RefSeq; NP_477324.1; NM_057976.3.
DR AlphaFoldDB; Q03372; -.
DR SMR; Q03372; -.
DR BioGRID; 69563; 21.
DR IntAct; Q03372; 15.
DR STRING; 7227.FBpp0084807; -.
DR iPTMnet; Q03372; -.
DR PaxDb; Q03372; -.
DR PRIDE; Q03372; -.
DR DNASU; 45285; -.
DR EnsemblMetazoa; FBtr0085441; FBpp0084807; FBgn0000492.
DR GeneID; 45285; -.
DR KEGG; dme:Dmel_CG1897; -.
DR CTD; 45285; -.
DR FlyBase; FBgn0000492; Dr.
DR VEuPathDB; VectorBase:FBgn0000492; -.
DR eggNOG; KOG0492; Eukaryota.
DR GeneTree; ENSGT00940000169520; -.
DR HOGENOM; CLU_041724_0_0_1; -.
DR InParanoid; Q03372; -.
DR OMA; WPGARQM; -.
DR OrthoDB; 858478at2759; -.
DR PhylomeDB; Q03372; -.
DR BioGRID-ORCS; 45285; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 45285; -.
DR PRO; PR:Q03372; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0000492; Expressed in wing disc and 63 other tissues.
DR Genevisible; Q03372; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:FlyBase.
DR GO; GO:0007420; P:brain development; IMP:FlyBase.
DR GO; GO:0007417; P:central nervous system development; NAS:FlyBase.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; TAS:FlyBase.
DR GO; GO:0007450; P:dorsal/ventral pattern formation, imaginal disc; IMP:FlyBase.
DR GO; GO:0007398; P:ectoderm development; TAS:FlyBase.
DR GO; GO:0048598; P:embryonic morphogenesis; IBA:GO_Central.
DR GO; GO:0021782; P:glial cell development; IMP:FlyBase.
DR GO; GO:0007485; P:imaginal disc-derived male genitalia development; IMP:FlyBase.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR GO; GO:0007517; P:muscle organ development; TAS:FlyBase.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:FlyBase.
DR GO; GO:0007400; P:neuroblast fate determination; TAS:FlyBase.
DR GO; GO:0007389; P:pattern specification process; NAS:FlyBase.
DR GO; GO:0042659; P:regulation of cell fate specification; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007419; P:ventral cord development; TAS:FlyBase.
DR GO; GO:0035309; P:wing and notum subfield formation; IMP:FlyBase.
DR GO; GO:0035222; P:wing disc pattern formation; IMP:FlyBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..515
FT /note="Muscle segmentation homeobox"
FT /id="PRO_0000049079"
FT DNA_BIND 421..480
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 90..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 142..185
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 214..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 307..342
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..76
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 150..170
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..270
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 68..515
FT /note="Missing: In Msh4-4; abnormalities in sense organ
FT precursor (SOP) cells and their progeny. Chordotonal organs
FT contain fewer neurons and glial cells and migration of the
FT progeny cells derived from the SOPs in embryos are
FT significantly desynchronized among different segments."
FT /evidence="ECO:0000269|PubMed:28716930"
FT CONFLICT 27
FT /note="S -> T (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 54
FT /note="G -> A (in Ref. 1; CAA59680)"
FT /evidence="ECO:0000305"
FT CONFLICT 86
FT /note="L -> V (in Ref. 1; CAA59680)"
FT /evidence="ECO:0000305"
FT CONFLICT 153
FT /note="A -> V (in Ref. 1; CAA59680)"
FT /evidence="ECO:0000305"
FT CONFLICT 337
FT /note="G -> A (in Ref. 1; CAA59680)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 515 AA; 54250 MW; FA3C35BC99590CBD CRC64;
MLKLSPASMT VTGLRQTMTS PTVPPSSNTP AGNLIITSSS SNSGSNSGSN MSSGNMTSSN
LTNLSPSHPA GLNALASPTS PSALLLAHQQ HLLQQHQQHQ QQQQQQQQAA ALQLAAVHPP
AHHLHKTTSR LSNFSVASLL ADTRPRTPPN QAADGPQNLT SSAATSPISQ ASSTPPPPPA
SAAAQVPANT FHPAAVAHHA HLLQAAHAAA AAHAQHQAMA AQLRQQQQQA DARANSPPAS
TSSTPSSTPL GSALGSQGNV ASTPAKNERH SPLGSHTDSE LEYDEEMLQD HEADHDEEED
SIVDIEDMNA DDSPRSTPDG LDGSGKSLES PHGPPPGSHM QSTILSPAAL ASGHVPIRPT
PFSALAAAAV AWTGMGGGVP WPGTRQMPPF GPPGMFPGAG FGGDANEPPR IKCNLRKHKP
NRKPRTPFTT QQLLSLEKKF REKQYLSIAE RAEFSSSLRL TETQVKIWFQ NRRAKAKRLQ
EAEIEKIKMA ALGRGAPGAQ WAMAGYFHPS LMHLG
