HMUD1_BPSAV
ID HMUD1_BPSAV Reviewed; 220 AA.
AC E1XT70;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 1.
DT 03-AUG-2022, entry version 29.
DE RecName: Full=5-hmdU DNA kinase 1 {ECO:0000303|PubMed:34522950};
DE AltName: Full=5-hydroxymethyluracil DNA kinase {ECO:0000303|PubMed:34522950};
DE AltName: Full=P-loop kinase {ECO:0000303|PubMed:34522950};
DE AltName: Full=gp67 {ECO:0000303|PubMed:34522950};
GN ORFNames=Vi01_061c {ECO:0000312|EMBL:CBW37929.1};
OS Salmonella phage ViI.
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Ackermannviridae; Cvivirinae; Kuttervirus.
OX NCBI_TaxID=1987993;
OH NCBI_TaxID=90370; Salmonella typhi.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20817773; DOI=10.1128/jb.00659-10;
RA Pickard D., Toribio A.L., Petty N.K., van Tonder A., Yu L., Goulding D.,
RA Barrell B., Rance R., Harris D., Wetter M., Wain J., Choudhary J.,
RA Thomson N., Dougan G.;
RT "A conserved acetyl esterase domain targets diverse bacteriophages to the
RT Vi capsular receptor of Salmonella enterica serovar Typhi.";
RL J. Bacteriol. 192:5746-5754(2010).
RN [2]
RP FUNCTION.
RX PubMed=29555775; DOI=10.1073/pnas.1714812115;
RA Lee Y.J., Dai N., Walsh S.E., Mueller S., Fraser M.E., Kauffman K.M.,
RA Guan C., Correa I.R. Jr., Weigele P.R.;
RT "Identification and biosynthesis of thymidine hypermodifications in the
RT genomic DNA of widespread bacterial viruses.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:E3116-E3125(2018).
RN [3]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=34522950; DOI=10.1093/nar/gkab781;
RA Lee Y.J., Dai N., Mueller S.I., Guan C., Parker M.J., Fraser M.E.,
RA Walsh S.E., Sridar J., Mulholland A., Nayak K., Sun Z., Lin Y.C.,
RA Comb D.G., Marks K., Gonzalez R., Dowling D.P., Bandarian V., Saleh L.,
RA Correa I.R., Weigele P.R.;
RT "Pathways of thymidine hypermodification.";
RL Nucleic Acids Res. 0:0-0(2021).
CC -!- FUNCTION: Phosphorylates 5-hydroxymethyluracil (5hmdU) into 5-
CC phosphomethyl-2'-deoxyuridine (5- PmdU) on DNA as a step in the pathway
CC leading to thymidine hypermodifications in the viral genome
CC (PubMed:34522950). The phosphate is added internally to the DNA polymer
CC (PubMed:34522950). As a final result of the pathway of
CC hypermodification, 5-aminoethoxy-2'-deoxymethyluridine (5-NeOmdU)
CC substitutes for about 40% of the thymidines in the viral DNA
CC (PubMed:34522950, PubMed:29555775). These modifications probably
CC prevent degradation of viral genome by the host restriction-
CC modification antiviral defense system (PubMed:34522950).
CC {ECO:0000269|PubMed:29555775, ECO:0000269|PubMed:34522950}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-hydroxymethyl-dUMP in DNA + ATP = 5-phosphomethyl-dUMP in
CC DNA + ADP + H(+); Xref=Rhea:RHEA:71543, Rhea:RHEA-COMP:18039,
CC Rhea:RHEA-COMP:18041, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:190917, ChEBI:CHEBI:190918, ChEBI:CHEBI:456216;
CC Evidence={ECO:0000269|PubMed:34522950};
CC -!- SIMILARITY: Belongs to the thymidylate kinase family. 5-hmdU DNA kinase
CC subfamily. {ECO:0000305}.
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DR EMBL; FQ312032; CBW37929.1; -; Genomic_DNA.
DR Proteomes; UP000000339; Genome.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR040924; Ploopntkinase1.
DR Pfam; PF18748; Ploopntkinase1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Host-virus interaction; Reference proteome;
KW Restriction-modification system evasion by virus.
FT CHAIN 1..220
FT /note="5-hmdU DNA kinase 1"
FT /id="PRO_0000456268"
SQ SEQUENCE 220 AA; 24603 MW; 64BD245318A75647 CRC64;
MSYSLKGLLK RPVHLFVKPP AVEGEYPARG ELYYVKGSNG SGKSTVPSYL AENDPQAYVV
TYNGKIMLTV CPSYNIICIG KYDKSKSKGV DSLKDTEQML FALSIADQPE YLKYDVLFEG
IIPSTLLSSW IPRLTRPPRE LVVLFMDTPL ETCVSRVKSR NGGADFNESL VVEKWERVHD
HSQRHKGLFP TVPAGMMKSN GLTIEQAVFA FLNRDFGSID