ID   HMUDK_BPPAM             Reviewed;         189 AA.
AC   A0A0S0N9S9;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   17-FEB-2016, sequence version 1.
DT   03-AUG-2022, entry version 22.
DE   RecName: Full=5-hmdU DNA kinase {ECO:0000303|PubMed:34522950};
DE   AltName: Full=5-hydroxymethyluracil DNA kinase {ECO:0000303|PubMed:34522950};
DE   AltName: Full=P-loop kinase {ECO:0000303|PubMed:34522950};
DE   AltName: Full=gp49 {ECO:0000303|PubMed:34522950};
GN   ORFNames=PaMx11_49 {ECO:0000312|EMBL:ALH23723.1};
OS   Pseudomonas phage PaMx11.
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Abidjanvirus.
OX   NCBI_TaxID=1175657;
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=22504803; DOI=10.1128/aem.00065-12;
RA   Sepulveda-Robles O., Kameyama L., Guarneros G.;
RT   "High Diversity and Novel Species of Pseudomonas aeruginosa
RT   Bacteriophages.";
RL   Appl. Environ. Microbiol. 78:4510-4515(2012).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=34522950; DOI=10.1093/nar/gkab781;
RA   Lee Y.J., Dai N., Mueller S.I., Guan C., Parker M.J., Fraser M.E.,
RA   Walsh S.E., Sridar J., Mulholland A., Nayak K., Sun Z., Lin Y.C.,
RA   Comb D.G., Marks K., Gonzalez R., Dowling D.P., Bandarian V., Saleh L.,
RA   Correa I.R., Weigele P.R.;
RT   "Pathways of thymidine hypermodification.";
RL   Nucleic Acids Res. 0:0-0(2021).
CC   -!- FUNCTION: Phosphorylates 5-hydroxymethyluracil (5hmdU) into 5-
CC       phosphomethyl-2'-deoxyuridine (5- PmdU) on DNA as a step in the pathway
CC       leading to thymidine hypermodifications in the viral genome
CC       (PubMed:34522950). The phosphate is added internally to the DNA polymer
CC       (PubMed:34522950). As a final result of the pathway of
CC       hypermodification, 5-AcNmdU substitutes for a subset of thymidines in
CC       the viral DNA (PubMed:34522950). These modifications probably prevent
CC       degradation of viral genome by the host restriction-modification
CC       antiviral defense system (PubMed:34522950).
CC       {ECO:0000269|PubMed:34522950}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-hydroxymethyl-dUMP in DNA + ATP = 5-phosphomethyl-dUMP in
CC         DNA + ADP + H(+); Xref=Rhea:RHEA:71543, Rhea:RHEA-COMP:18039,
CC         Rhea:RHEA-COMP:18041, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:190917, ChEBI:CHEBI:190918, ChEBI:CHEBI:456216;
CC         Evidence={ECO:0000269|PubMed:34522950};
CC   -!- SIMILARITY: Belongs to the thymidylate kinase family. 5-hmdU DNA kinase
CC       subfamily. {ECO:0000305}.
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DR   EMBL; JQ067087; ALH23723.1; -; Genomic_DNA.
DR   RefSeq; YP_009196302.1; NC_028770.1.
DR   GeneID; 26623527; -.
DR   KEGG; vg:26623527; -.
DR   Proteomes; UP000204009; Genome.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Restriction-modification system evasion by virus.
FT   CHAIN           1..189
FT                   /note="5-hmdU DNA kinase"
FT                   /id="PRO_0000456270"
SQ   SEQUENCE   189 AA;  20933 MW;  5892ABF041BB3ACD CRC64;
     MAKQRVINIR GTSGSGKSTL IRRLVELYPE KEPVHVPDRK QPLFYKLRGD GLLPLSLLGH
     YETACGGCDT IPSMDRIYEL VRERLAEGDS VLYEGLLISA EVNRAVALHT DGFDLTVVRL
     NTPLELCVDS VNQRRWAKNP DKPGVNPKNT EAKFKQTLAT CKKLDAAGIP VVEADRDGAF
     HAIKQLLEA