ID   HMUI_BPSP1              Reviewed;         174 AA.
AC   P34081;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   23-FEB-2022, entry version 72.
DE   RecName: Full=DNA endonuclease I-HmuI;
DE            EC=3.1.-.-;
DE   AltName: Full=HNH homing endonuclease I-HmuI;
OS   Bacillus phage SP01 (Bacteriophage SP01).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Herelleviridae; Spounavirinae; Okubovirus.
OX   NCBI_TaxID=10685;
OH   NCBI_TaxID=1423; Bacillus subtilis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2119891; DOI=10.1016/0092-8674(90)90174-d;
RA   Goodrich-Blair H., Scarlato V., Gott J.M., Xu M.Q., Shub D.A.;
RT   "A self-splicing group I intron in the DNA polymerase gene of Bacillus
RT   subtilis bacteriophage SPO1.";
RL   Cell 63:417-424(1990).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS).
RX   PubMed=15313606; DOI=10.1016/j.jmb.2004.07.032;
RA   Shen B.W., Landthaler M., Shub D.A., Stoddard B.L.;
RT   "DNA binding and cleavage by the HNH homing endonuclease I-HmuI.";
RL   J. Mol. Biol. 342:43-56(2004).
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DR   EMBL; M37686; AAA64536.1; -; Genomic_DNA.
DR   PIR; A36077; A36077.
DR   RefSeq; YP_002300418.1; NC_011421.1.
DR   PDB; 1U3E; X-ray; 2.92 A; M=1-174.
DR   PDBsum; 1U3E; -.
DR   SMR; P34081; -.
DR   REBASE; 2628; I-HmuI.
DR   GeneID; 7009136; -.
DR   KEGG; vg:7009136; -.
DR   EvolutionaryTrace; P34081; -.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR044925; His-Me_finger_sf.
DR   InterPro; IPR003615; HNH_nuc.
DR   InterPro; IPR003647; Intron_nuc_1_rpt.
DR   InterPro; IPR010902; NUMOD4.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF13392; HNH_3; 1.
DR   Pfam; PF07463; NUMOD4; 1.
DR   SMART; SM00507; HNHc; 1.
DR   SMART; SM00497; IENR1; 1.
DR   SUPFAM; SSF54060; SSF54060; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Endonuclease; Hydrolase; Intron homing; Nuclease.
FT   CHAIN           1..174
FT                   /note="DNA endonuclease I-HmuI"
FT                   /id="PRO_0000106168"
FT   STRAND          3..5
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   TURN            10..12
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          13..16
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          21..26
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          28..30
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          41..46
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          49..54
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   HELIX           55..63
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          72..75
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   HELIX           85..87
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          88..91
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   HELIX           93..103
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   HELIX           109..118
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          123..126
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          132..136
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   HELIX           138..145
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   HELIX           149..156
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          159..161
FT                   /evidence="ECO:0007829|PDB:1U3E"
FT   STRAND          166..170
FT                   /evidence="ECO:0007829|PDB:1U3E"
SQ   SEQUENCE   174 AA;  19766 MW;  BBA60B1E00D8AE88 CRC64;
     MEWKDIKGYE GHYQVSNTGE VYSIKSGKTL KHQIPKDGYH RIGLFKGGKG KTFQVHRLVA
     IHFCEGYEEG LVVDHKDGNK DNNLSTNLRW VTQKINVENQ MSRGTLNVSK AQQIAKIKNQ
     KPIIVISPDG IEKEYPSTKC ACEELGLTRG KVTDVLKGHR IHHKGYTFRY KLNG