HMW_DROME
ID HMW_DROME Reviewed; 604 AA.
AC Q9VE87; Q8T0T7;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Protein hemingway {ECO:0000312|FlyBase:FBgn0038607};
GN Name=hmw {ECO:0000312|FlyBase:FBgn0038607};
GN ORFNames=CG7669 {ECO:0000312|FlyBase:FBgn0038607};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL39212.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL39212.1};
RC TISSUE=Head {ECO:0000312|EMBL:AAL39212.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=24554765; DOI=10.1091/mbc.e13-10-0616;
RA Soulavie F., Piepenbrock D., Thomas J., Vieillard J., Duteyrat J.L.,
RA Cortier E., Laurencon A., Goepfert M.C., Durand B.;
RT "hemingway is required for sperm flagella assembly and ciliary motility in
RT Drosophila.";
RL Mol. Biol. Cell 25:1276-1286(2014).
CC -!- FUNCTION: Involved in assembly and/or maintenance of motile cilia.
CC Required during spermatogenesis for axoneme elongation. Necessary for
CC optimal function of the chordotonal (hearing) organs.
CC {ECO:0000269|PubMed:24554765}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:24554765}. Perikaryon
CC {ECO:0000269|PubMed:24554765}. Cytoplasm {ECO:0000269|PubMed:24554765}.
CC -!- TISSUE SPECIFICITY: Detected in ciliated sensory neurons at all stages
CC of development, and in adult testis. {ECO:0000269|PubMed:24554765}.
CC -!- DEVELOPMENTAL STAGE: In the testis, expressed in spermatocytes and
CC elongating spermatids. {ECO:0000269|PubMed:24554765}.
CC -!- DISRUPTION PHENOTYPE: Viable with no gross coordination or gravitaxis
CC defects. Males are sterile with complete absence of mature spermatozoa,
CC while female fertility is unaffected. In developing spermatids,
CC axonemes fail to elongate normally and sperm individualization is
CC disrupted. Tubulin glycylation of spermatid axonemes is abnormal.
CC Hearing is moderately impaired. {ECO:0000269|PubMed:24554765}.
CC -!- SIMILARITY: Belongs to the CFAP97 family. {ECO:0000305}.
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DR EMBL; AE014297; AAF55540.2; -; Genomic_DNA.
DR EMBL; AY069067; AAL39212.1; -; mRNA.
DR RefSeq; NP_650714.1; NM_142457.3.
DR AlphaFoldDB; Q9VE87; -.
DR STRING; 7227.FBpp0082997; -.
DR PaxDb; Q9VE87; -.
DR PRIDE; Q9VE87; -.
DR EnsemblMetazoa; FBtr0083576; FBpp0082997; FBgn0038607.
DR GeneID; 42207; -.
DR KEGG; dme:Dmel_CG7669; -.
DR UCSC; CG7669-RA; d. melanogaster.
DR CTD; 42207; -.
DR FlyBase; FBgn0038607; hmw.
DR VEuPathDB; VectorBase:FBgn0038607; -.
DR eggNOG; ENOG502SDBI; Eukaryota.
DR HOGENOM; CLU_452187_0_0_1; -.
DR InParanoid; Q9VE87; -.
DR OMA; QMSEMIM; -.
DR OrthoDB; 779734at2759; -.
DR PhylomeDB; Q9VE87; -.
DR BioGRID-ORCS; 42207; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 42207; -.
DR PRO; PR:Q9VE87; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038607; Expressed in testis and 11 other tissues.
DR GO; GO:0005929; C:cilium; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
DR GO; GO:0050910; P:detection of mechanical stimulus involved in sensory perception of sound; IMP:FlyBase.
DR GO; GO:0044458; P:motile cilium assembly; IMP:FlyBase.
DR GO; GO:0007288; P:sperm axoneme assembly; IMP:FlyBase.
DR GO; GO:0007291; P:sperm individualization; IMP:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR InterPro; IPR038791; Cfap97/Hemingway.
DR InterPro; IPR029488; Hmw/CFAP97.
DR PANTHER; PTHR23035; PTHR23035; 1.
DR Pfam; PF13879; Hmw_CFAP97; 1.
PE 2: Evidence at transcript level;
KW Cell projection; Cilium biogenesis/degradation; Cytoplasm; Differentiation;
KW Reference proteome; Spermatogenesis.
FT CHAIN 1..604
FT /note="Protein hemingway"
FT /id="PRO_0000435956"
FT REGION 1..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 103..309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 359..387
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 544..585
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..38
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 136..181
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 196..212
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 238..259
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 604 AA; 67635 MW; 27984FF0D903D364 CRC64;
MSGAGEHSDE ESYGEESFEE DSESEVEVEE EEIEYIEPEE SKPSDALLLG ESDTQSESDV
KEEFLSGNPH ARRYLGARVV SYLSSSSDDE SQVVITKTVA EVNQLSLRVD TPPEDETPSV
RTLMPGSAHS RVKDEDEAED EDDDEGDGEN DNDNEDENDD EDAEVEEIEE EEEEIPGDED
DAQSDGSMGR QSADDSDEEE GTDVEVEQLE EDEPIVTAVC KKTVQKELPP PQKDIVSLVD
ASDNSSEHSV VTMCASGDET DNTLVKYPRL RKQQPLTAKD SQSLKEQEEP EEPEQPEENQ
TEEHMPTSQP DLARVRYLEQ QMTQMSELIM KSFQINGGAP NSQAFEQLAM ATEMLQQRSD
RRGDTESEMS SMTETTMTSA RSLGGGIPPL TMRMERSQEA LLTTRSSKLP RPKCRCPSES
DLIEHEQKLD MGHELPHDLA ANFGLGEGYL VDECGQGDGL MRHPQQRKPM ELNRRAVSGT
PSTFNSDGCE YQINVSRSRC SNDKINYKNL GRKSFSFTNP QVREIERQNQ ILLRKMMTVK
PTATIKASTS SIKINGPEKR QTPPAPRLSS AAVNRKKNQR QIDLDNDLLK RKLEAIGTRR
PQFK
