HMX_DROME
ID HMX_DROME Reviewed; 592 AA.
AC Q9VEI9; A4IJ52; Q0IGR0;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 3.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Homeobox protein Hmx;
DE Short=DHmx;
GN Name=Hmx; ORFNames=CG43748;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=11091080; DOI=10.1016/s0925-4773(00)00488-3;
RA Wang W., Lo P., Frasch M., Lufkin T.;
RT "Hmx: an evolutionary conserved homeobox gene family expressed in the
RT developing nervous system in mice and Drosophila.";
RL Mech. Dev. 99:123-137(2000).
RN [5]
RP EXPRESSION IN MOUSE.
RX PubMed=15363417; DOI=10.1016/j.devcel.2004.06.016;
RA Wang W., Grimmer J.F., Van De Water T.R., Lufkin T.;
RT "Hmx2 and Hmx3 homeobox genes direct development of the murine inner ear
RT and hypothalamus and can be functionally replaced by Drosophila Hmx.";
RL Dev. Cell 7:439-453(2004).
CC -!- FUNCTION: Transcription factor involved in specification of neuronal
CC cell types. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- DEVELOPMENTAL STAGE: Initiates at the cellular blastoderm stage in
CC distinct bilateral domains within the procephalic neuroectoderm.
CC Present in narrow stripes that cover dorsolateral positions at about
CC 75% egg length. During gastrulation, a second bilateral pair appears
CC posteriorly to the initial domains, from which they are separated by a
CC 3 to 4 cell wide gap. During germ band elongation, both the primary and
CC secondary domains of expression become wedge-shaped. During stage 9, a
CC third pair of bilateral domains appears anteriorly to the primary
CC domains. The 3 domains of expression may reflect the proposed
CC subdivision of the protocerebral primordium into an anterior, central,
CC and posterior/optic lobe domain, respectively. The existence of gaps
CC between the domains of expression suggests that it marks only a subset
CC of cells within each of the 3 protocerebral primordia. Following stage
CC 9, neuroblasts segregate from the domains and the central and posterior
CC domains split into smaller subdomains. During later events of brain
CC morphogenesis, the presence of 3 domains of expression can still be
CC discerned, and prominent expression in late stage embryos include the
CC optic lobe region. At late stage 11, it is also expressed in the
CC ventral neuroectoderm, where it appears in a small cluster of neuronal
CC precursors within each hemisegment. {ECO:0000269|PubMed:11091080}.
CC -!- MISCELLANEOUS: When expressed in mice, Hmx is able to functionally
CC replace both Hmx2 and Hmx3 mouse proteins.
CC -!- SIMILARITY: Belongs to the HMX homeobox family. {ECO:0000305}.
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DR EMBL; AE014297; AAF55432.3; -; Genomic_DNA.
DR EMBL; BT028828; ABI34209.1; -; mRNA.
DR EMBL; BT028860; ABI34241.1; -; mRNA.
DR EMBL; BT030410; ABO52830.1; -; mRNA.
DR RefSeq; NP_001287376.1; NM_001300447.1.
DR RefSeq; NP_732244.3; NM_169763.3.
DR AlphaFoldDB; Q9VEI9; -.
DR SMR; Q9VEI9; -.
DR BioGRID; 67143; 19.
DR IntAct; Q9VEI9; 2.
DR STRING; 7227.FBpp0303411; -.
DR PaxDb; Q9VEI9; -.
DR PRIDE; Q9VEI9; -.
DR DNASU; 42110; -.
DR EnsemblMetazoa; FBtr0330385; FBpp0303411; FBgn0264005.
DR EnsemblMetazoa; FBtr0346339; FBpp0312056; FBgn0264005.
DR GeneID; 42110; -.
DR KEGG; dme:Dmel_CG43748; -.
DR UCSC; CG34419-RC; d. melanogaster.
DR CTD; 42110; -.
DR FlyBase; FBgn0264005; Hmx.
DR VEuPathDB; VectorBase:FBgn0264005; -.
DR eggNOG; KOG0485; Eukaryota.
DR HOGENOM; CLU_461734_0_0_1; -.
DR InParanoid; Q9VEI9; -.
DR OMA; WAPQRMP; -.
DR OrthoDB; 858478at2759; -.
DR PhylomeDB; Q9VEI9; -.
DR BioGRID-ORCS; 42110; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 42110; -.
DR PRO; PR:Q9VEI9; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0264005; Expressed in procephalic neurogenic region (Drosophila) and 23 other tissues.
DR ExpressionAtlas; Q9VEI9; baseline and differential.
DR Genevisible; Q9VEI9; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Homeobox;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..592
FT /note="Homeobox protein Hmx"
FT /id="PRO_0000341381"
FT DNA_BIND 468..527
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 248..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 305..473
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..72
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..155
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 309..353
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 363..412
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 435..452
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 592 AA; 62609 MW; 18CBAC853B3DC72E CRC64;
MSSSEAEVDI SVVSSPEPSP LGAAARDSPL LARSPARSAD GSSPPATPTH RSNTPNTAAG
TPTTSASGGN HFHHSPSGGA VPPAVLHHHL QHHLSSLGSH PVALHHALHT FGHLHPAHGA
THPALLQHAL TPTGQQQQQQ LQVQQQHQQS HVERLSPPMK SPERNGGDEV QGHSLNNNNS
KALNHNNTCA SAAAAAAAAA VAAANLSNNS NDSNGSSGGG GGKSTTGSNG FTSFSISSIL
SRSEPAKKNG AAGLITPIPQ LPQPGAGGPQ DAAMLSRLGF ISQWGALAGR YAALCPPGWP
WAPQRLPFHS PTHDSSSTNT TENPPSPTSM SPNNNNNNSN TTASSNQASK SPSHHPLHPL
YHPLGSQQQQ QQQQQHQQHP QQQQHPQQQQ QQHPHQPTTP TSSSSSGGGS SLTHHPHPHL
TGSHGGYLLP SSSNESDEEG EEIIEEDDGT DGPSDSSSPH GDGNSKRKKK TRTVFSRAQV
FQLESTFDLK RYLSSSERAG LAASLRLTET QVKIWFQNRR NKWKRQLAAE LEAANMANMA
HAAQRLVRVP VLYHDGTTAG FVPPPPPHHH PMQYYAAARN TSPPRPPLSS LV
