ID   HN324_CYRHA             Reviewed;          35 AA.
AC   P0CH70; P0CH69;
DT   02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=U1-theraphotoxin-Hhn1a;
DE            Short=U1-TRTX-Hhn1a;
DE   AltName: Full=Hainantoxin F3-24.71 {ECO:0000303|PubMed:20192277};
DE   Contains:
DE     RecName: Full=Hainantoxin-VI {ECO:0000303|Ref.2};
DE              Short=HNTX-VI {ECO:0000303|Ref.2};
DE     AltName: Full=Peptide F6-25.12 {ECO:0000303|PubMed:20192277};
OS   Cyriopagopus hainanus (Chinese bird spider) (Haplopelma hainanum).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Haplopelma.
OX   NCBI_TaxID=209901;
RN   [1]
RP   PROTEIN SEQUENCE, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=20192277; DOI=10.1021/pr1000016;
RA   Tang X., Zhang Y., Hu W., Xu D., Tao H., Yang X., Li Y., Jiang L.,
RA   Liang S.;
RT   "Molecular diversification of peptide toxins from the tarantula Haplopelma
RT   hainanum (Ornithoctonus hainana) venom based on transcriptomic, peptidomic,
RT   and genomic analyses.";
RL   J. Proteome Res. 9:2550-2564(2010).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-34, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RA   Pan J.Y., Hu W.J., Liang S.P.;
RT   "Purification, sequencing and characterization of hainantoxin-VI, a
RT   neurotoxin from the Chinese bird spider Selenocosmia hainana.";
RL   Dong Wu Xue Yan Jiu 23:280-283(2002).
RN   [3]
RP   FUNCTION.
RX   PubMed=20506577; DOI=10.1631/jzus.b0900393;
RA   Wang R.L., Yi S., Liang S.P.;
RT   "Mechanism of action of two insect toxins huwentoxin-III and hainantoxin-VI
RT   on voltage-gated sodium channels.";
RL   J. Zhejiang Univ. Sci. B 11:451-457(2010).
CC   -!- FUNCTION: [Hainantoxin-VI]: Gating-modifier toxin that dose-dependently
CC       inhibits inactivation of voltage-gated sodium channels and reduces the
CC       peak of sodium current in cockroach DUM neurons (PubMed:20506577). In
CC       vivo, reversibly paralyzes cockroaches for several hours, paralyzes rat
CC       after intracerebroventricular injection and blocks the neuromuscular
CC       transmission of the isolated rat phrenic nerve-diaphragm preparation
CC       (Ref.2). {ECO:0000269|PubMed:20506577, ECO:0000269|Ref.2}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20192277}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: [U1-theraphotoxin-Hhn1a]: Mass=4057.6; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:20192277};
CC   -!- MASS SPECTROMETRY: [Hainantoxin-VI]: Mass=3998.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:20192277};
CC   -!- MASS SPECTROMETRY: [Hainantoxin-VI]: Mass=3998.49; Method=MALDI;
CC       Evidence={ECO:0000269|Ref.2};
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 24 (Hwtx-6)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0CH70; -.
DR   SMR; P0CH70; -.
DR   ArachnoServer; AS002021; U1-theraphotoxin-Hhn1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   Pfam; PF07740; Toxin_12; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Secreted; Toxin.
FT   PEPTIDE         1..35
FT                   /note="U1-theraphotoxin-Hhn1a"
FT                   /evidence="ECO:0000269|PubMed:20192277"
FT                   /id="PRO_0000400718"
FT   PEPTIDE         1..34
FT                   /note="Hainantoxin-VI"
FT                   /evidence="ECO:0000269|PubMed:20192277, ECO:0000269|Ref.2"
FT                   /id="PRO_0000434820"
FT   DISULFID        2..16
FT                   /evidence="ECO:0000250"
FT   DISULFID        9..21
FT                   /evidence="ECO:0000250"
FT   DISULFID        15..28
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   35 AA;  4062 MW;  0BD5FE6489533182 CRC64;
     ECKYLWGTCE KDEHCCEHLG CNKKHGWCGW DGTFG