HNF1B_RAT
ID HNF1B_RAT Reviewed; 557 AA.
AC P23899;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Hepatocyte nuclear factor 1-beta;
DE Short=HNF-1-beta;
DE Short=HNF-1B;
DE AltName: Full=Transcription factor 2;
DE Short=TCF-2;
DE AltName: Full=Variant hepatic nuclear factor 1;
DE Short=vHNF1;
GN Name=Hnf1b; Synonyms=Hnf-1b, Tcf2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Liver;
RX PubMed=1673926; DOI=10.1002/j.1460-2075.1991.tb07665.x;
RA Rey-Campos J., Chouard T., Yaniv M., Cereghini S.;
RT "vHNF1 is a homeoprotein that activates transcription and forms
RT heterodimers with HNF1.";
RL EMBO J. 10:1445-1457(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=16641100; DOI=10.1073/pnas.0600895103;
RA Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.;
RT "Quantitative phosphoproteomics of vasopressin-sensitive renal cells:
RT regulation of aquaporin-2 phosphorylation at two sites.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49; SER-75 AND SER-80, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Transcription factor that binds to the inverted palindrome
CC 5'-GTTAATNATTAAC-3' (By similarity). Binds to the FPC element in the
CC cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional
CC activity is increased by coactivator PCBD1 (By similarity).
CC {ECO:0000250|UniProtKB:P35680, ECO:0000250|UniProtKB:Q03365}.
CC -!- SUBUNIT: Binds DNA as a dimer. Can form homodimer or heterodimer with
CC HNF1-alpha (By similarity). Interacts (via HNF-p1 domain) with PCBD1;
CC the interaction increases its transactivation activity (By similarity).
CC {ECO:0000250|UniProtKB:P35680}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=VHNF1-A;
CC IsoId=P23899-1; Sequence=Displayed;
CC Name=2; Synonyms=VHNF1-B;
CC IsoId=P23899-2; Sequence=VSP_002257;
CC -!- TISSUE SPECIFICITY: Liver, kidney and intestine.
CC -!- SIMILARITY: Belongs to the HNF1 homeobox family. {ECO:0000305}.
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DR EMBL; X56546; CAA39886.1; -; mRNA.
DR EMBL; BC081826; AAH81826.1; -; mRNA.
DR PIR; S15342; S15342.
DR RefSeq; NP_037235.1; NM_013103.2. [P23899-1]
DR AlphaFoldDB; P23899; -.
DR BMRB; P23899; -.
DR SMR; P23899; -.
DR STRING; 10116.ENSRNOP00000046025; -.
DR iPTMnet; P23899; -.
DR PhosphoSitePlus; P23899; -.
DR GeneID; 25640; -.
DR KEGG; rno:25640; -.
DR CTD; 6928; -.
DR RGD; 3830; Hnf1b.
DR VEuPathDB; HostDB:ENSRNOG00000002598; -.
DR HOGENOM; CLU_035503_0_0_1; -.
DR InParanoid; P23899; -.
DR OMA; DDTCSEP; -.
DR PhylomeDB; P23899; -.
DR PRO; PR:P23899; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000002598; Expressed in kidney and 15 other tissues.
DR ExpressionAtlas; P23899; baseline and differential.
DR Genevisible; P23899; RN.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0005667; C:transcription regulator complex; ISO:RGD.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:RGD.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:RGD.
DR GO; GO:0042802; F:identical protein binding; IPI:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:RGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:RGD.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISO:RGD.
DR GO; GO:0009952; P:anterior/posterior pattern specification; ISO:RGD.
DR GO; GO:0048754; P:branching morphogenesis of an epithelial tube; ISO:RGD.
DR GO; GO:0032922; P:circadian regulation of gene expression; IDA:RGD.
DR GO; GO:0048557; P:embryonic digestive tract morphogenesis; ISO:RGD.
DR GO; GO:0048598; P:embryonic morphogenesis; IDA:RGD.
DR GO; GO:0031018; P:endocrine pancreas development; ISS:UniProtKB.
DR GO; GO:0007492; P:endoderm development; ISO:RGD.
DR GO; GO:0001714; P:endodermal cell fate specification; ISO:RGD.
DR GO; GO:0050673; P:epithelial cell proliferation; ISO:RGD.
DR GO; GO:0060429; P:epithelium development; ISO:RGD.
DR GO; GO:0048806; P:genitalia development; ISS:UniProtKB.
DR GO; GO:0061017; P:hepatoblast differentiation; ISO:RGD.
DR GO; GO:0070365; P:hepatocyte differentiation; IEP:RGD.
DR GO; GO:0030902; P:hindbrain development; ISO:RGD.
DR GO; GO:0001826; P:inner cell mass cell differentiation; ISO:RGD.
DR GO; GO:0030073; P:insulin secretion; ISO:RGD.
DR GO; GO:0001822; P:kidney development; ISS:UniProtKB.
DR GO; GO:0060993; P:kidney morphogenesis; ISO:RGD.
DR GO; GO:0001889; P:liver development; ISO:RGD.
DR GO; GO:1900200; P:mesenchymal cell apoptotic process involved in metanephros development; IEA:Ensembl.
DR GO; GO:0072177; P:mesonephric duct development; ISO:RGD.
DR GO; GO:0072181; P:mesonephric duct formation; ISO:RGD.
DR GO; GO:0072164; P:mesonephric tubule development; ISO:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:RGD.
DR GO; GO:0061296; P:negative regulation of mesenchymal cell apoptotic process involved in mesonephric nephron morphogenesis; ISO:RGD.
DR GO; GO:1900212; P:negative regulation of mesenchymal cell apoptotic process involved in metanephros development; ISO:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0072176; P:nephric duct development; ISO:RGD.
DR GO; GO:0072179; P:nephric duct formation; ISO:RGD.
DR GO; GO:0007219; P:Notch signaling pathway; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:RGD.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0039020; P:pronephric nephron tubule development; ISO:RGD.
DR GO; GO:0048793; P:pronephros development; ISO:RGD.
DR GO; GO:0065004; P:protein-DNA complex assembly; IMP:RGD.
DR GO; GO:0072095; P:regulation of branch elongation involved in ureteric bud branching; ISO:RGD.
DR GO; GO:0035565; P:regulation of pronephros size; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0030111; P:regulation of Wnt signaling pathway; ISO:RGD.
DR GO; GO:0009743; P:response to carbohydrate; IEP:RGD.
DR GO; GO:0009749; P:response to glucose; ISO:RGD.
DR GO; GO:0014070; P:response to organic cyclic compound; IEP:RGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEP:RGD.
DR GO; GO:0060677; P:ureteric bud elongation; ISO:RGD.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR039066; HNF-1.
DR InterPro; IPR006899; HNF-1_N.
DR InterPro; IPR044869; HNF-1_POU.
DR InterPro; IPR023219; HNF1_dimer_N_dom_sf.
DR InterPro; IPR006897; HNF1b_C.
DR InterPro; IPR044866; HNF_P1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR PANTHER; PTHR11568; PTHR11568; 1.
DR Pfam; PF04814; HNF-1_N; 1.
DR Pfam; PF04812; HNF-1B_C; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF100957; SSF100957; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS51937; HNF_P1; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS51936; POU_4; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Homeobox; Nucleus;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..557
FT /note="Hepatocyte nuclear factor 1-beta"
FT /id="PRO_0000049124"
FT DOMAIN 1..32
FT /note="HNF-p1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01286"
FT DOMAIN 93..188
FT /note="POU-specific atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01285"
FT DNA_BIND 231..311
FT /note="Homeobox; HNF1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..31
FT /note="Dimerization"
FT /evidence="ECO:0000250"
FT REGION 66..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 324..370
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 49
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 52
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P27889"
FT MOD_RES 75
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 80
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT VAR_SEQ 183..208
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:1673926"
FT /id="VSP_002257"
SQ SEQUENCE 557 AA; 61371 MW; 7729E78C458B6DBC CRC64;
MVSKLTSLQQ ELLSALLSSG VTKEVLIQAL EELLPSPNFG VKLETLPLSP GSGADLDTKP
VFHTLTNGHA KGRLSGDEGS EDGDDYDTPP ILKELQALNT EEAAEQRAEV DRMLSEDPWR
AAKMIKGYMQ QHNIPQREVV DVTGLNQSHL SQHLNKGTPM KTQKRAALYT WYVRKQREIL
RQFNQTVQSS GNMTDKSSQD QLLFLFPEFS QQNQGPGQSE DACSEPTNKK MRRNRFKWGP
ASQQILYQAY DRQKNPSKEE REALVEECNR AECLQRGVSP SKAHGLGSNL VTEVRVYNWF
ANRRKEEAFR QKLAMDAYSS NQTHNLNPLL THGSPHHQPS SSPPNKLSGV RYSQPGNNEV
TSSSTISHHG NSAMVTSQSV LQQVSPASLD PGHSLLSPDS KMISVSGGGL PPVSTLTNIH
SLSHHNPQQS QNLIMTPLSG VMAIAQSLNT SQAQGVPVIN SVASSLAALQ PVQFSQQLHS
PHQQPLMQQS PGSHMAQQPF MAAVTQLQNS HMYAHKQEPP QYSHTSRFPS AMVVTDTSSI
NTLTSMSSSK QCPLQAW
