HNF4A_XENLA
ID HNF4A_XENLA Reviewed; 464 AA.
AC Q91766; Q8AVK3;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Hepatocyte nuclear factor 4-alpha;
DE Short=HNF-4-alpha;
DE AltName: Full=Nuclear receptor subfamily 2 group A member 1;
GN Name=hnf4a; Synonyms=hnf4, nr2a1;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC TISSUE=Liver;
RX PubMed=8808405; DOI=10.1016/0925-4773(95)00460-2;
RA Holewa B., Pogge von Strandmann E., Zapp D., Lorenz P., Ryffel G.U.;
RT "Transcriptional hierarchy in Xenopus embryogenesis: HNF4 a maternal factor
RT involved in the developmental activation of the gene encoding the tissue
RT specific transcription factor HNF1 alpha (LFB1).";
RL Mech. Dev. 54:45-57(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcriptional regulator; binds and activates the promoter
CC for the HNF1-alpha gene (PubMed:8808405). Potential initiator of a
CC transcriptional cascade within a subset of cells committed to a
CC specific developmental program (PubMed:8808405). Could be a determinant
CC for asymmetry in early development (PubMed:8808405). May play a role in
CC the regulation of the circadian clock (By similarity).
CC {ECO:0000250|UniProtKB:P41235, ECO:0000269|PubMed:8808405}.
CC -!- SUBUNIT: Homodimerization is required for HNF4-alpha to bind to its
CC recognition site. {ECO:0000250|UniProtKB:P41235}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in liver and kidney.
CC -!- DEVELOPMENTAL STAGE: Maternal protein which is distributed within an
CC animal-to-vegetal gradient in the embryo. Present throughout the entire
CC development.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:P41235}.
CC -!- MISCELLANEOUS: Binds fatty acids. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR2
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA85763.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z37526; CAA85763.1; ALT_INIT; mRNA.
DR EMBL; BC042224; AAH42224.1; -; mRNA.
DR RefSeq; NP_001080070.1; NM_001086601.1.
DR AlphaFoldDB; Q91766; -.
DR SMR; Q91766; -.
DR DNASU; 379762; -.
DR GeneID; 379762; -.
DR KEGG; xla:379762; -.
DR CTD; 379762; -.
DR Xenbase; XB-GENE-864872; hnf4a.S.
DR OrthoDB; 622732at2759; -.
DR Proteomes; UP000186698; Chromosome 9_10S.
DR Bgee; 379762; Expressed in intestine and 6 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0003707; F:nuclear steroid receptor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0070365; P:hepatocyte differentiation; IEA:InterPro.
DR GO; GO:0055088; P:lipid homeostasis; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0042752; P:regulation of circadian rhythm; ISS:UniProtKB.
DR GO; GO:0050796; P:regulation of insulin secretion; IEA:InterPro.
DR GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR GO; GO:0003323; P:type B pancreatic cell development; IEA:InterPro.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR039067; HNF4A.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR000003; Retinoid-X_rcpt/HNF4.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR24083:SF41; PTHR24083:SF41; 1.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00545; RETINOIDXR.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Biological rhythms; DNA-binding; Metal-binding; Nucleus;
KW Receptor; Reference proteome; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..464
FT /note="Hepatocyte nuclear factor 4-alpha"
FT /id="PRO_0000053561"
FT DOMAIN 147..376
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 57..132
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 60..80
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 96..120
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 410..433
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 367..375
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:P41235"
FT CONFLICT 335
FT /note="E -> D (in Ref. 2; AAH42224)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 464 AA; 51967 MW; B42ABD572989E484 CRC64;
MRLSKALIDM DMADYTEALD PAYTTLEFEN MQVLSIGTDT STSDVTSLSA SNSIGINSLC
AICGDRATGK HYGASSCDGC KGFFRRSVRK NHMYSCRFSR QCVVDKDKRN QCRYCRLKKC
FRAGMKKEAV QNERDRISTR RSSYEDSSLP SINVLIQAEV LSQQITSSVG VLNTDIRGKK
IACIIDVCDS MKQQLLVLVE WAKYIPAFCE LPLDDQVALL RAHAGEHLLL GATKRSMMFK
DILLLGNDRL IPRNCPELEV GRVAVRILDE LVLPFQELQI DDNEYACLKA IIFFDPDAKG
LSDPTKIKRM RYQVQVSLED YINDRQYDSR GRFGELLLLL PTLQSITWQM IEQIQFVKLF
GMAKIDNLLQ EMLLGGSANE ASHTHHHLHP HLVQDHLATN VIVANNTLPS QLHNGQMSTP
ETPQPSPPAG SGAEQYKIVH GTIASINKQP TSIPQSTITK QEAM
