HNF6_RAT
ID HNF6_RAT Reviewed; 465 AA.
AC P70512; O88755;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 25-MAY-2022, entry version 146.
DE RecName: Full=Hepatocyte nuclear factor 6;
DE Short=HNF-6;
DE AltName: Full=One cut domain family member 1;
DE AltName: Full=One cut homeobox 1;
GN Name=Onecut1; Synonyms=Hnf6, Hnf6a;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM ALPHA).
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=8790352; DOI=10.1073/pnas.93.18.9460;
RA Lemaigre F.P., Durviaux S.M., Truong O., Lannoy V.J., Hsuan J.J.,
RA Rousseau G.G.;
RT "Hepatocyte nuclear factor 6, a transcription factor that contains a novel
RT type of homeodomain and a single cut domain.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:9460-9464(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
RC STRAIN=Wistar;
RX PubMed=9593691; DOI=10.1074/jbc.273.22.13552;
RA Lannoy V.J., Buerglin T.R., Rousseau G.G., Lemaigre F.P.;
RT "Isoforms of hepatocyte nuclear factor-6 differ in DNA-binding properties,
RT contain a bifunctional homeodomain, and define the new ONECUT class of
RT homeodomain proteins.";
RL J. Biol. Chem. 273:13552-13562(1998).
RN [3]
RP CHARACTERIZATION.
RX PubMed=8887657; DOI=10.1128/mcb.16.11.6273;
RA Samadani U., Costa R.H.;
RT "The transcriptional activator hepatocyte nuclear factor 6 regulates liver
RT gene expression.";
RL Mol. Cell. Biol. 16:6273-6284(1996).
CC -!- FUNCTION: Transcriptional activator. Binds the consensus sequence 5'-
CC DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B
CC and TTR. Important for liver genes transcription. The affinity of HNF-
CC 6-alpha and HNF-6-beta for DNA differs depending on the target
CC sequence.
CC -!- SUBUNIT: Binds DNA as a monomer.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Alpha;
CC IsoId=P70512-1; Sequence=Displayed;
CC Name=Beta;
CC IsoId=P70512-2; Sequence=VSP_002312;
CC -!- TISSUE SPECIFICITY: Expressed in liver, brain, spleen and testis.
CC -!- SIMILARITY: Belongs to the CUT homeobox family. {ECO:0000305}.
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DR EMBL; X96553; CAA65389.1; -; Genomic_DNA.
DR EMBL; Y14933; CAA75150.1; -; mRNA.
DR RefSeq; NP_073162.1; NM_022671.2. [P70512-1]
DR PDB; 2D5V; X-ray; 2.00 A; A/B=289-444.
DR PDBsum; 2D5V; -.
DR AlphaFoldDB; P70512; -.
DR BMRB; P70512; -.
DR SMR; P70512; -.
DR STRING; 10116.ENSRNOP00000010739; -.
DR GeneID; 25231; -.
DR KEGG; rno:25231; -.
DR UCSC; RGD:2811; rat. [P70512-1]
DR CTD; 3175; -.
DR RGD; 2811; Onecut1.
DR eggNOG; KOG2252; Eukaryota.
DR InParanoid; P70512; -.
DR OrthoDB; 709082at2759; -.
DR EvolutionaryTrace; P70512; -.
DR PRO; PR:P70512; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0003682; F:chromatin binding; ISO:RGD.
DR GO; GO:0003677; F:DNA binding; IMP:RGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:BHF-UCL.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:RGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0009653; P:anatomical structure morphogenesis; ISO:RGD.
DR GO; GO:0030183; P:B cell differentiation; ISO:RGD.
DR GO; GO:0030154; P:cell differentiation; ISO:RGD.
DR GO; GO:0045165; P:cell fate commitment; ISO:RGD.
DR GO; GO:0060271; P:cilium assembly; ISO:RGD.
DR GO; GO:0031018; P:endocrine pancreas development; ISO:RGD.
DR GO; GO:0007492; P:endoderm development; ISO:RGD.
DR GO; GO:0002064; P:epithelial cell development; ISO:RGD.
DR GO; GO:0006006; P:glucose metabolic process; IMP:RGD.
DR GO; GO:0001889; P:liver development; ISO:RGD.
DR GO; GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; ISO:RGD.
DR GO; GO:0007219; P:Notch signaling pathway; ISO:RGD.
DR GO; GO:0031016; P:pancreas development; ISO:RGD.
DR GO; GO:0030335; P:positive regulation of cell migration; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:BHF-UCL.
DR GO; GO:0001952; P:regulation of cell-matrix adhesion; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0048536; P:spleen development; ISO:RGD.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR003350; CUT_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR Pfam; PF02376; CUT; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM01109; CUT; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS51042; CUT; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Alternative splicing; DNA-binding; Homeobox;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..465
FT /note="Hepatocyte nuclear factor 6"
FT /id="PRO_0000202404"
FT DNA_BIND 283..369
FT /note="CUT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00374"
FT DNA_BIND 385..444
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 15..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 442..465
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..140
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 446..465
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 368
FT /note="A -> AESAMGGSVPSLRITSGGPQLSVPPLP (in isoform Beta)"
FT /evidence="ECO:0000303|PubMed:9593691"
FT /id="VSP_002312"
FT HELIX 294..307
FT /evidence="ECO:0007829|PDB:2D5V"
FT HELIX 312..318
FT /evidence="ECO:0007829|PDB:2D5V"
FT HELIX 324..332
FT /evidence="ECO:0007829|PDB:2D5V"
FT HELIX 337..339
FT /evidence="ECO:0007829|PDB:2D5V"
FT HELIX 344..355
FT /evidence="ECO:0007829|PDB:2D5V"
FT HELIX 358..364
FT /evidence="ECO:0007829|PDB:2D5V"
FT HELIX 394..406
FT /evidence="ECO:0007829|PDB:2D5V"
FT HELIX 412..422
FT /evidence="ECO:0007829|PDB:2D5V"
FT HELIX 426..438
FT /evidence="ECO:0007829|PDB:2D5V"
SQ SEQUENCE 465 AA; 51067 MW; BD651267FD7AC896 CRC64;
MNAQLTMEAI GELHGVSHEP VPAPADLLGG SPHARSSVGH RGSHLPPAHP RSMGMASLLD
GGSGGSDYHH HHRAPEHSLA GPLHPTMTMA CETPPGMSMP TTYTTLTPLQ PLPPISTVSD
KFPHHHHHHH HHHHPHHHQR LAGNVSGSFT LMRDERGLAS MNNLYTPYHK DVAGMGQSLS
PLSGSGLGSI HNSQQGLPHY AHPGAAMPTD KMLTPNDFEA HHPAMLGRHG EQHLTPTSAG
MVPINGLPPH HPHAHLNAQG HGQLLGTARE PNPSVTGAQV SNGSNSGQME EINTKEVAQR
ITTELKRYSI PQAIFAQRVL CRSQGTLSDL LRNPKPWSKL KSGRETFRRM WKWLQEPEFQ
RMSALRLAAC KRKEQEHGKD RGNTPKKPRL VFTDVQRRTL HAIFKENKRP SKELQITISQ
QLGLELSTVS NFFMNARRRS LDKWQDEGSS NSGNSSSSSS TCTKA
