ID   HNH78_ECOLX             Reviewed;         216 AA.
AC   P0DV92;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Retron Ec78 putative HNH endonuclease {ECO:0000303|PubMed:33157039};
DE   AltName: Full=TIGR02646 family protein {ECO:0000305};
GN   ORFNames=Ga0100609_101824;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1] {ECO:0000312|EMBL:JHRW01000018}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=102598;
RA   Hazen T.H., Donnenberg M., Nataro J., Kaper J., Rasko D.;
RT   "Emergence of novel diverse phylogenomic lineages of EPEC.";
RL   Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION IN ANTIVIRAL DEFENSE, AND IDENTIFICATION AS A RETRON.
RC   STRAIN=102598;
RX   PubMed=33157039; DOI=10.1016/j.cell.2020.09.065;
RA   Millman A., Bernheim A., Stokar-Avihail A., Fedorenko T., Voichek M.,
RA   Leavitt A., Oppenheimer-Shaanan Y., Sorek R.;
RT   "Bacterial Retrons Function In Anti-Phage Defense.";
RL   Cell 183:1551-1561(2020).
CC   -!- FUNCTION: Putative HNH endonuclease component of antiviral defense
CC       system retron Ec78, composed of a non-coding RNA (ncRNA), a reverse
CC       transcriptase (RT), a probable ATPase and this protein. Expression of
CC       retron Ec78 confers protection against bacteriophage T5. At
CC       multiplicity of infection (MOI) of 0.02 cultures slow growth when
CC       infected with T5 but do not collapse, at MOI 2 cultures enter growth
CC       stasis. {ECO:0000269|PubMed:33157039}.
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DR   EMBL; JHRW01000018; -; NOT_ANNOTATED_CDS; Genomic_DNA.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..216
FT                   /note="Retron Ec78 putative HNH endonuclease"
FT                   /id="PRO_0000456027"
SQ   SEQUENCE   216 AA;  24789 MW;  93D0C29C80E5541E CRC64;
     MKELARLESP EILDQYTAGQ NDWMEIDQSA VWPKLTEMQG EFCAYCECRL NRRHIEHFRP
     RGKFPALTFI WSNLFGSCGD SKKSGGWSRC GIYKDNGAGA YNADDLIKPD EENPDDYLLF
     LTTGEVVPAI GLTGRALKKA QETIRVFNLN GDIKLLGSRR TAVQAIMPNV EYLYSLLEEF
     EEDDWNEMLR DELEKIESDE FKTALKHAWT SNQEFA