ID   HNSP_SALTI              Reviewed;         134 AA.
AC   Q9L5H8;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=DNA-binding protein H-NS, plasmid;
DE   AltName: Full=Plasmid R27-encoded H-HS {ECO:0000303|PubMed:19521501};
DE            Short=H-NS-R27 {ECO:0000303|PubMed:19521501};
GN   Name=hns;
OS   Salmonella typhi.
OG   Plasmid IncHI1 R27.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=90370;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=IncHI1 R27;
RX   PubMed=10773089; DOI=10.1093/nar/28.10.2177;
RA   Sherburne C.K., Lawley T.D., Gilmour M.W., Blattner F.R., Burland V.,
RA   Grotbeck E., Rose D.J., Taylor D.E.;
RT   "The complete DNA sequence and analysis of R27, a large IncHI plasmid from
RT   Salmonella typhi that is temperature sensitive for transfer.";
RL   Nucleic Acids Res. 28:2177-2186(2000).
RN   [2]
RP   FUNCTION, AND DNA-BINDING.
RC   PLASMID=IncHI1 R27;
RX   PubMed=19521501; DOI=10.1371/journal.pgen.1000513;
RA   Banos R.C., Vivero A., Aznar S., Garcia J., Pons M., Madrid C., Juarez A.;
RT   "Differential regulation of horizontally acquired and core genome genes by
RT   the bacterial modulator H-NS.";
RL   PLoS Genet. 5:E1000513-E1000513(2009).
CC   -!- FUNCTION: A DNA-binding protein implicated in transcriptional
CC       repression and chromosome organization and compaction. Binds DNA,
CC       modifying gene expression, especially non-core genes. Does not regulate
CC       the same set of genes as its chromosomal counterpart (tested in
CC       S.typhimurium strain SL1344 / SV5015, chromosomal H-NS protein is AC
CC       A0A0H3NBY9). Thus it has a not-completely overlapping set of gene
CC       targets compared to its chromosomal homolog; many of these target genes
CC       are either plasmid-encoded or acquired by horizontally transferred
CC       genes (HTG). This protein can function in the absence of H-NS-
CC       modulating protein Hha (either chromosomal or plasmid-encoded),
CC       although many HTG genes are regulated by an H-NS/Hha complex
CC       (PubMed:19521501). Binds nucleation sites in AT-rich DNA and bridges
CC       them, forming higher-order nucleoprotein complexes and condensing the
CC       chromosome (By similarity). A subset of genes are repressed by H-NS in
CC       association with Hha and/or Cnu (ydgT) (By similarity).
CC       {ECO:0000250|UniProtKB:P0ACF8, ECO:0000269|PubMed:19521501}.
CC   -!- SUBUNIT: Homodimer that oligomerizes on DNA into higher-order complexes
CC       that form bridges between disparate regions of DNA compacting it.
CC       Interacts with Hha, YdgT and StpA. {ECO:0000250|UniProtKB:P0ACF8}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid
CC       {ECO:0000250|UniProtKB:P0ACF8}.
CC   -!- MISCELLANEOUS: This protein is encoded on incompatibility group H
CC       (IncHI) plasmid R27, a temperature-sensitive conjugative plasmid.
CC       {ECO:0000305|PubMed:10773089}.
CC   -!- SIMILARITY: Belongs to the histone-like protein H-NS family.
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DR   EMBL; AF250878; AAF70002.1; -; Genomic_DNA.
DR   RefSeq; NP_058377.1; NC_002305.1.
DR   RefSeq; WP_010892345.1; NC_002305.1.
DR   AlphaFoldDB; Q9L5H8; -.
DR   SMR; Q9L5H8; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.287.1050; -; 1.
DR   Gene3D; 4.10.430.10; -; 1.
DR   InterPro; IPR027444; H-NS_C_dom.
DR   InterPro; IPR037150; H-NS_C_dom_sf.
DR   InterPro; IPR001801; Histone_HNS.
DR   InterPro; IPR027454; Histone_HNS_N.
DR   Pfam; PF00816; Histone_HNS; 1.
DR   PIRSF; PIRSF002096; HnS; 1.
DR   SMART; SM00528; HNS; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; DNA-binding; Plasmid; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..134
FT                   /note="DNA-binding protein H-NS, plasmid"
FT                   /id="PRO_0000436895"
FT   DNA_BIND        112..117
FT                   /evidence="ECO:0000250|UniProtKB:P0A1S2"
FT   REGION          77..96
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          23..67
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   134 AA;  15208 MW;  AFFA374805240ECB CRC64;
     MSEALKSLNN IRTLRAQGRE LPLEILEELL EKLSVVVEER RQEESSKEAE LKARLEKIES
     LRQLMLEDGI DPEELLSSFS AKSGAPKKVR EPRPAKYKYT DVNGETKTWT GQGRTPKALA
     EQLEAGKKLD DFLI