ID   HNS_SALTS               Reviewed;         137 AA.
AC   A0A0H3NBY9;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   03-AUG-2022, entry version 24.
DE   RecName: Full=DNA-binding protein H-NS;
GN   Name=hns; OrderedLocusNames=SL1344_1682;
OS   Salmonella typhimurium (strain SL1344).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=216597;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SL1344;
RX   PubMed=22538806; DOI=10.1073/pnas.1201061109;
RA   Kroger C., Dillon S.C., Cameron A.D., Papenfort K., Sivasankaran S.K.,
RA   Hokamp K., Chao Y., Sittka A., Hebrard M., Handler K., Colgan A.,
RA   Leekitcharoenphon P., Langridge G.C., Lohan A.J., Loftus B., Lucchini S.,
RA   Ussery D.W., Dorman C.J., Thomson N.R., Vogel J., Hinton J.C.;
RT   "The transcriptional landscape and small RNAs of Salmonella enterica
RT   serovar Typhimurium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:E1277-E1286(2012).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND DNA-BINDING.
RC   STRAIN=SL1344 / SV5015;
RX   PubMed=19521501; DOI=10.1371/journal.pgen.1000513;
RA   Banos R.C., Vivero A., Aznar S., Garcia J., Pons M., Madrid C., Juarez A.;
RT   "Differential regulation of horizontally acquired and core genome genes by
RT   the bacterial modulator H-NS.";
RL   PLoS Genet. 5:E1000513-E1000513(2009).
CC   -!- FUNCTION: A DNA-binding protein implicated in transcriptional
CC       repression (PubMed:19521501) and chromosome organization and
CC       compaction. Modifies gene expression, regardless of whether the gene is
CC       chromosomal or was acquired by horizontal gene transfer
CC       (PubMed:19521501). Binds nucleation sites in AT-rich DNA and bridges
CC       them, forming higher-order nucleoprotein complexes and condensing the
CC       chromosome. As many horizontally transferred genes are AT-rich, it
CC       plays a central role in silencing foreign genes. A subset of genes are
CC       repressed by H-NS in association with Hha and/or Cnu (ydgT) (By
CC       similarity). {ECO:0000250|UniProtKB:P0ACF8,
CC       ECO:0000269|PubMed:19521501}.
CC   -!- SUBUNIT: Homodimer that oligomerizes on DNA into higher-order complexes
CC       that form bridges between disparate regions of DNA compacting it.
CC       Interacts with Hha, YdgT and StpA. {ECO:0000250|UniProtKB:P0ACF8}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid
CC       {ECO:0000250|UniProtKB:P0ACF8}.
CC   -!- DISRUPTION PHENOTYPE: Altered expression of a significant number of
CC       genes; expression of an R27-plasmid encoded hns (AC Q9L5H8) restores
CC       wild-type expression to only a subset of these genes.
CC       {ECO:0000269|PubMed:19521501}.
CC   -!- SIMILARITY: Belongs to the histone-like protein H-NS family.
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DR   EMBL; FQ312003; CBW17777.1; -; Genomic_DNA.
DR   RefSeq; WP_001287383.1; NZ_QASL01000001.1.
DR   AlphaFoldDB; A0A0H3NBY9; -.
DR   SMR; A0A0H3NBY9; -.
DR   EnsemblBacteria; CBW17777; CBW17777; SL1344_1682.
DR   GeneID; 66756227; -.
DR   KEGG; sey:SL1344_1682; -.
DR   PATRIC; fig|216597.6.peg.1859; -.
DR   HOGENOM; CLU_117503_0_0_6; -.
DR   OMA; NGVEKTW; -.
DR   BioCyc; SENT216597:SL1344_RS08745-MON; -.
DR   Proteomes; UP000008962; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.287.1050; -; 1.
DR   Gene3D; 4.10.430.10; -; 1.
DR   InterPro; IPR027444; H-NS_C_dom.
DR   InterPro; IPR037150; H-NS_C_dom_sf.
DR   InterPro; IPR001801; Histone_HNS.
DR   InterPro; IPR027454; Histone_HNS_N.
DR   Pfam; PF00816; Histone_HNS; 1.
DR   PIRSF; PIRSF002096; HnS; 1.
DR   SMART; SM00528; HNS; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; DNA-binding; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..137
FT                   /note="DNA-binding protein H-NS"
FT                   /id="PRO_0000436894"
FT   DNA_BIND        112..117
FT                   /evidence="ECO:0000250|UniProtKB:P0A1S2"
FT   COILED          13..65
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   137 AA;  15543 MW;  A9620E5CC7DD3EB1 CRC64;
     MSEALKILNN IRTLRAQARE CTLETLEEML EKLEVVVNER REEESAAAAE VEERTRKLQQ
     YREMLIADGI DPNELLNSMA AAKSGTKAKR AARPAKYSYV DENGETKTWT GQGRTPAVIK
     KAMEEQGKQL EDFLIKE