ID   HOBOT_DROME             Reviewed;         644 AA.
AC   P12258;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Transposable element Hobo transposase;
GN   Name=T;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16453744; DOI=10.1002/j.1460-2075.1986.tb04690.x;
RA   Streck R.D., Macgaffey J.E., Beckendorf S.K.;
RT   "The structure of hobo transposable elements and their insertion sites.";
RL   EMBO J. 5:3615-3623(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   VARIANT 521-THR--GLU-541 DEL.
RX   PubMed=1651170; DOI=10.1016/0092-8674(81)90010-6;
RA   Calvi B.R., Hong T.J., Findley S.D., Gelbart W.M.;
RT   "Evidence for a common evolutionary origin of inverted repeat transposons
RT   in Drosophila and plants: hobo, Activator, and Tam3.";
RL   Cell 66:465-471(1991).
CC   -!- FUNCTION: Essential for hobo transposase activity.
CC       {ECO:0000269|PubMed:1651170}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:1651170}.
CC   -!- POLYMORPHISM: The number of repeats is highly polymorphic and varies
CC       among different strains.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA51465.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X04705; CAA28410.1; -; Genomic_DNA.
DR   EMBL; M69216; AAA51465.1; ALT_INIT; Genomic_DNA.
DR   PIR; A25684; A25684.
DR   AlphaFoldDB; P12258; -.
DR   SMR; P12258; -.
DR   PRIDE; P12258; -.
DR   FlyBase; FBgn0014191; hobo\T.
DR   PRO; PR:P12258; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   InterPro; IPR008906; HATC_C_dom.
DR   InterPro; IPR018473; Hermes_transposase_DNA-db.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR003656; Znf_BED.
DR   Pfam; PF10683; DBD_Tnp_Hermes; 1.
DR   Pfam; PF05699; Dimer_Tnp_hAT; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50808; ZF_BED; 1.
PE   4: Predicted;
KW   DNA integration; DNA recombination; DNA-binding; Metal-binding; Nucleus;
KW   Repeat; Transposable element; Zinc; Zinc-finger.
FT   CHAIN           1..644
FT                   /note="Transposable element Hobo transposase"
FT                   /id="PRO_0000084025"
FT   REPEAT          521..523
FT                   /note="1"
FT   REPEAT          524..526
FT                   /note="2"
FT   REPEAT          527..529
FT                   /note="3"
FT   REPEAT          530..532
FT                   /note="4"
FT   REPEAT          533..535
FT                   /note="5"
FT   REPEAT          536..538
FT                   /note="6"
FT   REPEAT          539..541
FT                   /note="7"
FT   REPEAT          542..544
FT                   /note="8"
FT   REPEAT          545..547
FT                   /note="9"
FT   REPEAT          548..550
FT                   /note="10"
FT   ZN_FING         73..131
FT                   /note="BED-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00027"
FT   REGION          514..560
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          521..550
FT                   /note="10 X 3 AA tandem repeats of T-P-E"
FT   COMPBIAS        524..544
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        545..560
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         521..541
FT                   /note="Missing"
FT                   /evidence="ECO:0000269|PubMed:1651170"
FT   CONFLICT        576
FT                   /note="P -> L (in Ref. 2; AAA51465)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        638..644
FT                   /note="ELKECFP -> AAERVFSLAGNIITEKRNRLCPKSVDSLLFLHSYYKNLNNS
FT                   Q (in Ref. 2; AAA51465)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   644 AA;  73388 MW;  83168CE40F64D3A6 CRC64;
     MAPYIMIVEF LCLWSSVSAV NCPFFVFYDA ITSLLGFSII WKPKEKVTIM AEAADFVKNK
     INNGTYSVAN KHKGKSVIWS ILCDILKEDE TVLDGWLFCR QCQKVLKFLH KNTSNLSRHK
     CCLTLRRPTE LKIVSENDKK VAIEKCTQWV VQDCRPFSAV TGAGFKNLVK FFLQIGAIYG
     EQVDVDDLLP DPTTLSRKAK SDAEEKRSLI SSEIKKAVDS GRASATVDMW TDQYVQRNFL
     GITFHYEKEF KLCDMILGLK SMNFQKSTAE NILMKIKGLF SEFNVENIDN VKFVTDRGAN
     IKKALEGNTR LNCSSHLLSN VLEKSFNEAN ELKKIVKSCK KIVKYCKKSN LQHTLETTLK
     SACPTRWNSN YKMMKSILDN WRSVDKILGE ADIHVDFNKS SLKVVVDILG DFERIFKKLQ
     TSSSPSICFV LPSISKILEL CEPNILDLSA AALLKERILE NIRKIWMANL SIWHKAAFFL
     YPPAAHLQEE DILEIKVFCI SQIQVPISYT LSLESTETPR TPETPETPET PETPETPETP
     ETPETPETPE SLESPNLFPK KNKTISSENE FFFPKPVTES NSNFNESPLD EIERYIRQRV
     PLSQNFEVIE WWKNNANLYP QLSKLALKLL SIPASSAELK ECFP