ID   HOFN_ECOLI              Reviewed;         179 AA.
AC   P64634; P45752; Q2M759;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=DNA utilization protein HofN;
GN   Name=hofN; Synonyms=yrfC; OrderedLocusNames=b3394, JW3357;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / W3110 / ZK126;
RX   PubMed=16707682; DOI=10.1128/jb.01974-05;
RA   Palchevskiy V., Finkel S.E.;
RT   "Escherichia coli competence gene homologs are essential for competitive
RT   fitness and the use of DNA as a nutrient.";
RL   J. Bacteriol. 188:3902-3910(2006).
CC   -!- FUNCTION: Required for the use of extracellular DNA as a nutrient.
CC       {ECO:0000269|PubMed:16707682}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Mutants are unable to use DNA as a sole carbon
CC       and energy source and show decreased competitive fitness when
CC       cocultured with wild-type cells. {ECO:0000269|PubMed:16707682}.
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DR   EMBL; U18997; AAA58191.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76419.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77897.1; -; Genomic_DNA.
DR   PIR; E65134; E65134.
DR   RefSeq; NP_417853.1; NC_000913.3.
DR   RefSeq; WP_001069315.1; NZ_SSZK01000008.1.
DR   AlphaFoldDB; P64634; -.
DR   BioGRID; 4263039; 7.
DR   STRING; 511145.b3394; -.
DR   PaxDb; P64634; -.
DR   PRIDE; P64634; -.
DR   EnsemblBacteria; AAC76419; AAC76419; b3394.
DR   EnsemblBacteria; BAE77897; BAE77897; BAE77897.
DR   GeneID; 66672726; -.
DR   GeneID; 947898; -.
DR   KEGG; ecj:JW3357; -.
DR   KEGG; eco:b3394; -.
DR   PATRIC; fig|1411691.4.peg.3336; -.
DR   EchoBASE; EB2760; -.
DR   eggNOG; COG3166; Bacteria.
DR   HOGENOM; CLU_081304_2_0_6; -.
DR   OMA; HFTLRIT; -.
DR   PhylomeDB; P64634; -.
DR   BioCyc; EcoCyc:G7738-MON; -.
DR   PRO; PR:P64634; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015976; P:carbon utilization; IMP:EcoCyc.
DR   GO; GO:0006308; P:DNA catabolic process; IMP:EcoCyc.
DR   GO; GO:0043683; P:type IV pilus assembly; IBA:GO_Central.
DR   GO; GO:0043107; P:type IV pilus-dependent motility; IBA:GO_Central.
DR   InterPro; IPR007813; PilN.
DR   Pfam; PF05137; PilN; 1.
PE   4: Predicted;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..179
FT                   /note="DNA utilization protein HofN"
FT                   /id="PRO_0000169539"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   179 AA;  20796 MW;  2A7D86A4B36B10C8 CRC64;
     MNPPINFLPW RQQRRTAFLR FWLLMFVAPL LLAVGITLIL RLTGSAEARI DAVLLQAEQQ
     LARSLQITKP RLLEQQQLRE QRSQRQRQRQ FTRDWQSALE ALAALLPEHA WLTTISWQQG
     TLEIKGLTTS ITALNALETS LRQDASFHLN QRGATQQDAQ GRWQFEYQLT RKVSDEHVL