HOFO_ECOLI
ID HOFO_ECOLI Reviewed; 146 AA.
AC P45751; Q2M758;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=DNA utilization protein HofO;
GN Name=hofO; Synonyms=yrfB; OrderedLocusNames=b3393, JW3356;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / W3110 / ZK126;
RX PubMed=16707682; DOI=10.1128/jb.01974-05;
RA Palchevskiy V., Finkel S.E.;
RT "Escherichia coli competence gene homologs are essential for competitive
RT fitness and the use of DNA as a nutrient.";
RL J. Bacteriol. 188:3902-3910(2006).
CC -!- FUNCTION: Required for the use of extracellular DNA as a nutrient.
CC {ECO:0000269|PubMed:16707682}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Mutants are unable to use DNA as a sole carbon
CC and energy source and show decreased competitive fitness when
CC cocultured with wild-type cells. {ECO:0000269|PubMed:16707682}.
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DR EMBL; U18997; AAA58190.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76418.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77898.1; -; Genomic_DNA.
DR PIR; D65134; D65134.
DR RefSeq; NP_417852.1; NC_000913.3.
DR RefSeq; WP_001055759.1; NZ_LN832404.1.
DR AlphaFoldDB; P45751; -.
DR SMR; P45751; -.
DR BioGRID; 4262484; 6.
DR DIP; DIP-12919N; -.
DR IntAct; P45751; 4.
DR STRING; 511145.b3393; -.
DR PaxDb; P45751; -.
DR PRIDE; P45751; -.
DR EnsemblBacteria; AAC76418; AAC76418; b3393.
DR EnsemblBacteria; BAE77898; BAE77898; BAE77898.
DR GeneID; 947899; -.
DR KEGG; ecj:JW3356; -.
DR KEGG; eco:b3393; -.
DR PATRIC; fig|1411691.4.peg.3337; -.
DR EchoBASE; EB2759; -.
DR eggNOG; ENOG5032UHA; Bacteria.
DR HOGENOM; CLU_117126_0_0_6; -.
DR OMA; WLARCGM; -.
DR PhylomeDB; P45751; -.
DR BioCyc; EcoCyc:G7737-MON; -.
DR PRO; PR:P45751; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015976; P:carbon utilization; IMP:EcoCyc.
DR GO; GO:0006308; P:DNA catabolic process; IMP:EcoCyc.
PE 4: Predicted;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..146
FT /note="DNA utilization protein HofO"
FT /id="PRO_0000169538"
FT TRANSMEM 20..37
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 146 AA; 16846 MW; 96548622C7D0374B CRC64;
MNMFFDWWFA TSPRLRQLCW AFWLLMLVTL IFLSSTHHEE RDALIRLRAS HHQQWAALYR
LVDTAPFSEE KTLPFSPLDF QLSGAQLVSW HPSAQGGELA LKTLWEAVPS AFTRLAERNV
SVSRFSLSVE GDDLLFTLQL ETPHEG
