HOFQ_ECOLI
ID HOFQ_ECOLI Reviewed; 412 AA.
AC P34749; Q2M756;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=DNA utilization protein HofQ;
DE Flags: Precursor;
GN Name=hofQ; Synonyms=hopQ; OrderedLocusNames=b3391, JW3354;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1309529; DOI=10.1128/jb.174.2.525-529.1992;
RA Lobner-Olesen A., Boye E., Marinus M.G.;
RT "Identification of the gene (aroK) encoding shikimic acid kinase I of
RT Escherichia coli.";
RL J. Bacteriol. 174:525-529(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP IDENTIFICATION.
RX PubMed=7901733; DOI=10.1111/j.1365-2958.1993.tb01744.x;
RA Martin P.R., Hobbs M., Free P.D., Jeske Y., Mattick J.S.;
RT "Characterization of pilQ, a new gene required for the biogenesis of type 4
RT fimbriae in Pseudomonas aeruginosa.";
RL Mol. Microbiol. 9:857-868(1993).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / W3110 / ZK126;
RX PubMed=11591672; DOI=10.1128/jb.183.21.6288-6293.2001;
RA Finkel S.E., Kolter R.;
RT "DNA as a nutrient: novel role for bacterial competence gene homologs.";
RL J. Bacteriol. 183:6288-6293(2001).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / W3110 / ZK126;
RX PubMed=16707682; DOI=10.1128/jb.01974-05;
RA Palchevskiy V., Finkel S.E.;
RT "Escherichia coli competence gene homologs are essential for competitive
RT fitness and the use of DNA as a nutrient.";
RL J. Bacteriol. 188:3902-3910(2006).
CC -!- FUNCTION: Required for the use of extracellular DNA as a nutrient
CC (PubMed:11591672). Could be the porin responsible for transport of DNA
CC across the outer membrane (PubMed:16707682).
CC {ECO:0000269|PubMed:11591672, ECO:0000305|PubMed:16707682}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Mutants show a stationary-phase competition
CC defect during coculture with wild-type cells and an inability to
CC utilize extracellular DNA as the sole source of carbon or energy. They
CC have no defects in DNA uptake by electroporation or when made
CC chemically competent (PubMed:11591672). {ECO:0000269|PubMed:11591672,
CC ECO:0000269|PubMed:16707682}.
CC -!- SIMILARITY: Belongs to the bacterial secretin family. PilQ subfamily.
CC {ECO:0000305}.
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DR EMBL; Z19601; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U18997; AAA58188.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76416.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77900.1; -; Genomic_DNA.
DR PIR; B65134; B65134.
DR RefSeq; NP_417850.1; NC_000913.3.
DR RefSeq; WP_000815987.1; NZ_SSZK01000008.1.
DR AlphaFoldDB; P34749; -.
DR SMR; P34749; -.
DR BioGRID; 4261394; 17.
DR IntAct; P34749; 6.
DR STRING; 511145.b3391; -.
DR PaxDb; P34749; -.
DR PRIDE; P34749; -.
DR EnsemblBacteria; AAC76416; AAC76416; b3391.
DR EnsemblBacteria; BAE77900; BAE77900; BAE77900.
DR GeneID; 947901; -.
DR KEGG; ecj:JW3354; -.
DR KEGG; eco:b3391; -.
DR PATRIC; fig|511145.12.peg.3484; -.
DR EchoBASE; EB2036; -.
DR eggNOG; COG4796; Bacteria.
DR HOGENOM; CLU_006756_2_1_6; -.
DR InParanoid; P34749; -.
DR OMA; QHTRREL; -.
DR PhylomeDB; P34749; -.
DR BioCyc; EcoCyc:EG12113-MON; -.
DR PRO; PR:P34749; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IMP:EcoliWiki.
DR GO; GO:0015976; P:carbon utilization; IMP:EcoCyc.
DR GO; GO:0006308; P:DNA catabolic process; IMP:EcoCyc.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR Gene3D; 3.30.1370.120; -; 1.
DR InterPro; IPR001775; GspD/PilQ.
DR InterPro; IPR005644; NolW-like.
DR InterPro; IPR038591; NolW-like_sf.
DR InterPro; IPR013355; Pilus_4_PilQ.
DR InterPro; IPR011662; Secretin/TonB_short_N.
DR InterPro; IPR004846; T2SS/T3SS.
DR InterPro; IPR004845; T2SS_GspD_CS.
DR Pfam; PF00263; Secretin; 1.
DR Pfam; PF03958; Secretin_N; 1.
DR PRINTS; PR00811; BCTERIALGSPD.
DR SMART; SM00965; STN; 1.
DR TIGRFAMs; TIGR02515; IV_pilus_PilQ; 1.
DR PROSITE; PS00875; T2SP_D; 1.
PE 3: Inferred from homology;
KW Cell outer membrane; Membrane; Reference proteome; Signal; Transport.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..412
FT /note="DNA utilization protein HofQ"
FT /id="PRO_0000013107"
SQ SEQUENCE 412 AA; 44716 MW; 99FEAEE2606682E5 CRC64;
MKQWIAALLL MLIPGVQAAK PQKVTLMVDD VPVAQVLQAL AEQEKLNLVV SPDVSGTVSL
HLTDVPWKQA LQTVVKSAGL ITRQEGNILS VHSIAWQNNN IARQEAEQAR AQANLPLENR
SITLQYADAG ELAKAGEKLL SAKGSMTVDK RTNRLLLRDN KTALSALEQW VAQMDLPVGQ
VELSAHIVTI NEKSLRELGV KWTLADAQHA GGVGQVTTLG SDLSVATATT HVGFNIGRIN
GRLLDLELSA LEQKQQLDII ASPRLLASHL QPASIKQGSE IPYQVSSGES GATSVEFKEA
VLGMEVTPTV LQKGRIRLKL HISQNVPGQV LQQADGEVLA IDKQEIETQV EVKSGETLAL
GGIFTRKNKS GQDSVPLLGD IPWFGQLFRH DGKEDERREL VVFITPRLVS SE
