HOKA_ECOLI
ID HOKA_ECOLI Reviewed; 50 AA.
AC P37305; Q2M7L8;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Protein HokA;
GN Name=hokA {ECO:0000303|PubMed:10361310}; Synonyms=yiaZ;
GN OrderedLocusNames=b4455, JW3526;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2404279; DOI=10.1073/pnas.87.1.283;
RA Goldstein J., Pollitt N.S., Inouye M.;
RT "Major cold shock protein of Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:283-287(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP IDENTIFICATION.
RX PubMed=7940673; DOI=10.1016/0968-0004(94)90067-1;
RA Borodovsky M., Koonin E.V., Rudd K.E.;
RT "New genes in old sequence: a strategy for finding genes in the bacterial
RT genome.";
RL Trends Biochem. Sci. 19:309-313(1994).
RN [6]
RP IDENTIFICATION.
RX PubMed=7984428; DOI=10.1093/nar/22.22.4756;
RA Borodovsky M., Rudd K.E., Koonin E.V.;
RT "Intrinsic and extrinsic approaches for detecting genes in a bacterial
RT genome.";
RL Nucleic Acids Res. 22:4756-4767(1994).
RN [7]
RP FUNCTION.
RC STRAIN=C / 1055, and K12 / MG1655 / ATCC 47076;
RX PubMed=10361310; DOI=10.1046/j.1365-2958.1999.01431.x;
RA Pedersen K., Gerdes K.;
RT "Multiple hok genes on the chromosome of Escherichia coli.";
RL Mol. Microbiol. 32:1090-1102(1999).
RN [8]
RP FUNCTION AS A TOXIN, AND INDUCTION.
RC STRAIN=K12 / BW25113;
RX PubMed=20105222; DOI=10.1111/j.1462-2920.2009.02147.x;
RA Kim Y., Wang X., Zhang X.S., Grigoriu S., Page R., Peti W., Wood T.K.;
RT "Escherichia coli toxin/antitoxin pair MqsR/MqsA regulate toxin CspD.";
RL Environ. Microbiol. 12:1105-1121(2010).
CC -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system
CC (Probable). When overexpressed kills cells within minutes; causes
CC collapse of the transmembrane potential and arrest of respiration
CC (PubMed:10361310, PubMed:20105222). Its toxic effect is probably
CC neutralized by antisense antitoxin RNA SokA (PubMed:10361310).
CC {ECO:0000269|PubMed:10361310, ECO:0000269|PubMed:20105222,
CC ECO:0000305|PubMed:10361310}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P0ACG4, ECO:0000305}; Single-pass membrane
CC protein {ECO:0000250|UniProtKB:P0ACG4}.
CC -!- INDUCTION: May be repressed by MqsA. {ECO:0000269|PubMed:20105222}.
CC -!- MISCELLANEOUS: Interrupted by an IS150 element, which silences the
CC locus in strain K12 MG1655. {ECO:0000305|PubMed:10361310}.
CC -!- SIMILARITY: Belongs to the Hok/Gef family. {ECO:0000305}.
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DR EMBL; M30139; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00039; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00096; AAT48191.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77738.1; -; Genomic_DNA.
DR RefSeq; WP_001135732.1; NZ_SSZK01000068.1.
DR RefSeq; YP_026229.1; NC_000913.3.
DR AlphaFoldDB; P37305; -.
DR BioGRID; 4259713; 12.
DR IntAct; P37305; 1.
DR STRING; 511145.b4455; -.
DR TCDB; 1.E.53.1.2; the toxic hok/gef protein (hok/gef) family.
DR PaxDb; P37305; -.
DR PRIDE; P37305; -.
DR EnsemblBacteria; AAT48191; AAT48191; b4455.
DR EnsemblBacteria; BAE77738; BAE77738; BAE77738.
DR GeneID; 2847732; -.
DR KEGG; ecj:JW3526; -.
DR KEGG; eco:b4455; -.
DR PATRIC; fig|83333.103.peg.4475; -.
DR EchoBASE; EB2250; -.
DR eggNOG; ENOG50337V7; Bacteria.
DR HOGENOM; CLU_177638_2_2_6; -.
DR OMA; LACDIKQ; -.
DR PhylomeDB; P37305; -.
DR BioCyc; EcoCyc:MON0-1605; -.
DR PRO; PR:P37305; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR000021; Hok/gef_toxin.
DR InterPro; IPR018084; Hok/gef_toxin_CS.
DR Pfam; PF01848; HOK_GEF; 1.
DR PRINTS; PR00281; HOKGEFTOXIC.
DR PROSITE; PS00556; HOK_GEF; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT CHAIN 1..50
FT /note="Protein HokA"
FT /id="PRO_0000199029"
FT TRANSMEM 7..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 1..2
FT /note="MP -> NA (in Ref. 2; M30139)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 50 AA; 5998 MW; A7E0BD75F45781AE CRC64;
MPQKYRLLSL IVICFTLLFF TWMIRDSLCE LHIKQESYEL AAFLACKLKE
