HOKC_ECOLI
ID HOKC_ECOLI Reviewed; 50 AA.
AC P0ACG4; P22982; P75618; Q2MCH5;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Toxic protein HokC;
DE AltName: Full=Protein Gef;
GN Name=hokC {ECO:0000303|PubMed:10361310};
GN Synonyms=gef {ECO:0000303|PubMed:2693900}; OrderedLocusNames=b4412, JW5002;
GN ORFNames=b0018.1;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2693900; DOI=10.1111/j.1365-2958.1989.tb00131.x;
RA Poulsen L.K., Larsen N.W., Molin S., Andersson P.;
RT "A family of genes encoding a cell-killing function may be conserved in all
RT Gram-negative bacteria.";
RL Mol. Microbiol. 3:1463-1472(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1630901; DOI=10.1093/nar/20.13.3305;
RA Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K.,
RA Mizobuchi K., Nakata A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 0-
RT 2.4 min region.";
RL Nucleic Acids Res. 20:3305-3308(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBUNIT, SUBCELLULAR LOCATION, DOMAIN, DISULFIDE BOND, MUTAGENESIS, AND
RP TOPOLOGY.
RX PubMed=1943700; DOI=10.1111/j.1365-2958.1991.tb01910.x;
RA Poulsen L.K., Refn A., Molin S., Andersson P.;
RT "Topographic analysis of the toxic Gef protein from Escherichia coli.";
RL Mol. Microbiol. 5:1627-1637(1991).
RN [6]
RP FUNCTION.
RC STRAIN=ECOR 24, and K12 / MG1655 / ATCC 47076;
RX PubMed=10361310; DOI=10.1046/j.1365-2958.1999.01431.x;
RA Pedersen K., Gerdes K.;
RT "Multiple hok genes on the chromosome of Escherichia coli.";
RL Mol. Microbiol. 32:1090-1102(1999).
CC -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system. When
CC overexpressed kills cells within minutes; causes collapse of the
CC transmembrane potential and arrest of respiration (PubMed:10361310).
CC Its toxic effect is probably neutralized by antisense antitoxin RNA
CC SokC (PubMed:10361310). {ECO:0000269|PubMed:10361310,
CC ECO:0000305|PubMed:10361310}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000269|PubMed:1943700}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:1943700};
CC Single-pass type II membrane protein.
CC -!- DOMAIN: The periplasmic portion confers toxicity; dimerization is not
CC required for toxicity. {ECO:0000269|PubMed:1943700}.
CC -!- MISCELLANEOUS: Interrupted by an IS186 element, which silences the
CC locus in strain K12 MG1655. {ECO:0000305|PubMed:10361310}.
CC -!- SIMILARITY: Belongs to the Hok/Gef family. {ECO:0000305}.
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DR EMBL; X17311; CAB37348.1; -; Genomic_DNA.
DR EMBL; U00096; AAT48122.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76031.1; -; Genomic_DNA.
DR RefSeq; WP_000809168.1; NZ_LN832404.1.
DR RefSeq; YP_025292.1; NC_000913.3.
DR AlphaFoldDB; P0ACG4; -.
DR SMR; P0ACG4; -.
DR BioGRID; 4259470; 3.
DR TCDB; 1.E.53.1.1; the toxic hok/gef protein (hok/gef) family.
DR PaxDb; P0ACG4; -.
DR PRIDE; P0ACG4; -.
DR EnsemblBacteria; AAT48122; AAT48122; b4412.
DR EnsemblBacteria; BAE76031; BAE76031; BAE76031.
DR GeneID; 2847744; -.
DR GeneID; 67416095; -.
DR KEGG; ecj:JW5002; -.
DR KEGG; eco:b4412; -.
DR PATRIC; fig|511145.12.peg.15; -.
DR EchoBASE; EB0368; -.
DR eggNOG; ENOG50339S1; Bacteria.
DR HOGENOM; CLU_177638_2_0_6; -.
DR PhylomeDB; P0ACG4; -.
DR BioCyc; EcoCyc:MON0-1564; -.
DR PRO; PR:P0ACG4; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042597; C:periplasmic space; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR InterPro; IPR000021; Hok/gef_toxin.
DR InterPro; IPR018084; Hok/gef_toxin_CS.
DR Pfam; PF01848; HOK_GEF; 1.
DR PRINTS; PR00281; HOKGEFTOXIC.
DR PROSITE; PS00556; HOK_GEF; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Disulfide bond; Membrane;
KW Reference proteome; Signal-anchor; Toxin-antitoxin system; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..50
FT /note="Toxic protein HokC"
FT /id="PRO_0000199031"
FT TOPO_DOM 1..5
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:1943700"
FT TRANSMEM 6..24
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000305"
FT TOPO_DOM 25..50
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:1943700"
FT MUTAGEN 15
FT /note="C->S: No action on toxicity."
FT /evidence="ECO:0000269|PubMed:1943700"
FT MUTAGEN 25
FT /note="T->K: Non-toxic."
FT /evidence="ECO:0000269|PubMed:1943700"
FT MUTAGEN 29
FT /note="L->P: Non-toxic."
FT /evidence="ECO:0000269|PubMed:1943700"
FT MUTAGEN 30
FT /note="C->S: Prevents dimer formation."
FT /evidence="ECO:0000269|PubMed:1943700"
FT MUTAGEN 41
FT /note="V->A: Non-toxic."
FT /evidence="ECO:0000269|PubMed:1943700"
FT MUTAGEN 49
FT /note="S->E: Non-toxic."
FT /evidence="ECO:0000269|PubMed:1943700"
SQ SEQUENCE 50 AA; 5502 MW; 9EC371A1043F682D CRC64;
MKQHKAMIVA LIVICITAVV AALVTRKDLC EVHIRTGQTE VAVFTAYESE
