HOKD_ECOLI
ID HOKD_ECOLI Reviewed; 51 AA.
AC P0ACG6; P07009; P77643; Q8X294;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Toxic protein HokD;
DE AltName: Full=Protein RelF;
GN Name=hokD {ECO:0000303|PubMed:10361310}; Synonyms=relF;
GN OrderedLocusNames=b1562, JW1554;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / CS520;
RX PubMed=2990907; DOI=10.1002/j.1460-2075.1985.tb03739.x;
RA Bech F.W., Joergensen S.T., Diderichsen B., Karlstroem O.H.;
RT "Sequence of the relB transcription unit from Escherichia coli and
RT identification of the relB gene.";
RL EMBO J. 4:1059-1066(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=K12;
RX PubMed=3019679; DOI=10.1002/j.1460-2075.1986.tb04459.x;
RA Gerdes K., Bech F.W., Joergensen S.T., Loebner-Olesen A., Rasmussen P.B.,
RA Atlung T., Boe L., Karlstrom O., Molin S., von Meyenburg K.;
RT "Mechanism of postsegregational killing by the hok gene product of the parB
RT system of plasmid R1 and its homology with the relF gene product of the E.
RT coli relB operon.";
RL EMBO J. 5:2023-2029(1986).
RN [6]
RP DISCUSSION OF SEQUENCE.
RX PubMed=10361310; DOI=10.1046/j.1365-2958.1999.01431.x;
RA Pedersen K., Gerdes K.;
RT "Multiple hok genes on the chromosome of Escherichia coli.";
RL Mol. Microbiol. 32:1090-1102(1999).
CC -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system
CC (Probable). When overexpressed kills cells within 2 minutes; causes
CC collapse of the transmembrane potential and arrest of respiration
CC (PubMed:3019679). {ECO:0000269|PubMed:3019679, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P0ACG4, ECO:0000305|PubMed:3019679}; Single-pass
CC membrane protein {ECO:0000250|UniProtKB:P0ACG4}.
CC -!- MISCELLANEOUS: Probably inactive in strain K12 MG1655 due to a genetic
CC locus rearrangement, the locus for its antisense antitoxin RNA is
CC missing. {ECO:0000305|PubMed:10361310}.
CC -!- SIMILARITY: Belongs to the Hok/Gef family. {ECO:0000305}.
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DR EMBL; X02405; CAA26252.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74635.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15261.1; -; Genomic_DNA.
DR PIR; E64911; QQECR5.
DR RefSeq; NP_416080.1; NC_000913.3.
DR RefSeq; WP_000813254.1; NZ_SSZK01000019.1.
DR AlphaFoldDB; P0ACG6; -.
DR SMR; P0ACG6; -.
DR BioGRID; 4260242; 117.
DR STRING; 511145.b1562; -.
DR PaxDb; P0ACG6; -.
DR PRIDE; P0ACG6; -.
DR EnsemblBacteria; AAC74635; AAC74635; b1562.
DR EnsemblBacteria; BAA15261; BAA15261; BAA15261.
DR GeneID; 60668608; -.
DR GeneID; 67415730; -.
DR GeneID; 948616; -.
DR KEGG; ecj:JW1554; -.
DR KEGG; eco:b1562; -.
DR PATRIC; fig|1411691.4.peg.700; -.
DR EchoBASE; EB1120; -.
DR eggNOG; ENOG50334M8; Bacteria.
DR HOGENOM; CLU_177638_2_0_6; -.
DR OMA; CEVRIRS; -.
DR PhylomeDB; P0ACG6; -.
DR BioCyc; EcoCyc:EG11130-MON; -.
DR PRO; PR:P0ACG6; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008219; P:cell death; IMP:EcoCyc.
DR InterPro; IPR000021; Hok/gef_toxin.
DR InterPro; IPR018084; Hok/gef_toxin_CS.
DR Pfam; PF01848; HOK_GEF; 1.
DR PRINTS; PR00281; HOKGEFTOXIC.
DR PROSITE; PS00556; HOK_GEF; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT CHAIN 1..51
FT /note="Toxic protein HokD"
FT /id="PRO_0000199033"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 37
FT /note="G -> D (in Ref. 1; CAA26252)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 51 AA; 5737 MW; 927EDBDE60B61A27 CRC64;
MKQQKAMLIA LIVICLTVIV TALVTRKDLC EVRIRTGQTE VAVFTAYEPE E
