ID   HOKE_ECO57              Reviewed;          50 AA.
AC   Q8XBW9; Q7AGS2;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 2.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Protein HokE;
GN   Name=hokE; OrderedLocusNames=Z0722, ECs0621;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system (By
CC       similarity). When overexpressed kills cells within minutes; causes
CC       collapse of the transmembrane potential and arrest of respiration (By
CC       similarity). Its toxic effect is probably neutralized by an antisense
CC       antitoxin Sok RNA (By similarity). {ECO:0000250|UniProtKB:P0ACG4}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P0ACG4}; Single-pass membrane protein
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Hok/Gef family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG54916.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAB34044.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE005174; AAG54916.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BA000007; BAB34044.1; ALT_INIT; Genomic_DNA.
DR   PIR; E90706; E90706.
DR   PIR; H85556; H85556.
DR   RefSeq; NP_308648.1; NC_002695.1.
DR   RefSeq; WP_000956465.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; Q8XBW9; -.
DR   SMR; Q8XBW9; -.
DR   STRING; 155864.EDL933_0649; -.
DR   EnsemblBacteria; AAG54916; AAG54916; Z0722.
DR   EnsemblBacteria; BAB34044; BAB34044; ECs_0621.
DR   GeneID; 66671144; -.
DR   GeneID; 916980; -.
DR   KEGG; ece:Z0722; -.
DR   KEGG; ecs:ECs_0621; -.
DR   PATRIC; fig|386585.9.peg.729; -.
DR   eggNOG; ENOG50336J3; Bacteria.
DR   HOGENOM; CLU_177638_3_0_6; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000021; Hok/gef_toxin.
DR   InterPro; IPR018084; Hok/gef_toxin_CS.
DR   Pfam; PF01848; HOK_GEF; 1.
DR   PRINTS; PR00281; HOKGEFTOXIC.
DR   PROSITE; PS00556; HOK_GEF; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT   CHAIN           1..50
FT                   /note="Protein HokE"
FT                   /id="PRO_0000199037"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   50 AA;  5592 MW;  8AD9BA232CEF6835 CRC64;
     MLTKYALVAV IVLCLTVLGF TLLVGDSLCE FTVKERNIEF KAVLAYEPKK